Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation
Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2021.1951896 |
| _version_ | 1819279498692001792 |
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| author | Fumin Yuan Ya-Min Li Zhuhui Wang |
| author_facet | Fumin Yuan Ya-Min Li Zhuhui Wang |
| author_sort | Fumin Yuan |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application and drug delivery, determination of the favorable storage conditions of EVs has been challenging. The understanding of the impact of storage conditions on EVs before and after isolation is still limited. Storage may change the size, number, contents, functions, and behaviors of EVs. Here, we summarized current studies about the stability of EVs in different conditions, focusing on temperatures, durations, and freezing and thawing cycles. –80 °C seems to remain the most favorable condition for storage of biofluids and isolated EVs, while isolated EVs may be stored at 4 °C shortly. Lyophilization is promising for storage of EV products. Challenges remain in the understanding of storage-mediated change in EVs and in the development of advanced preservation techniques of EVs. |
| first_indexed | 2024-12-24T00:28:52Z |
| format | Article |
| id | doaj.art-f03ee68a077140769d2bd5824951005e |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-24T00:28:52Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-f03ee68a077140769d2bd5824951005e2022-12-21T17:24:21ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642021-01-012811501150910.1080/10717544.2021.19518961951896Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolationFumin Yuan0Ya-Min Li1Zhuhui Wang2Department of Clinical Medicine, Grade 2018, Xiangya School of Medicine of Central South UniversityClinical Nursing Teaching and Research Section, Second Xiangya Hospital of Central South UniversityHunan Testing Institute for Medical DevicesExtracellular vesicles (EVs) are nanovesicles released by various cell types. EVs are known for cell-to-cell communications and have potent biological activities. Despite great progress in recent years for studies exploring the potentials of EVs for early disease detection, therapeutic application and drug delivery, determination of the favorable storage conditions of EVs has been challenging. The understanding of the impact of storage conditions on EVs before and after isolation is still limited. Storage may change the size, number, contents, functions, and behaviors of EVs. Here, we summarized current studies about the stability of EVs in different conditions, focusing on temperatures, durations, and freezing and thawing cycles. –80 °C seems to remain the most favorable condition for storage of biofluids and isolated EVs, while isolated EVs may be stored at 4 °C shortly. Lyophilization is promising for storage of EV products. Challenges remain in the understanding of storage-mediated change in EVs and in the development of advanced preservation techniques of EVs.http://dx.doi.org/10.1080/10717544.2021.1951896extracellular vesiclesexosomespreservationstoragenanomedicine |
| spellingShingle | Fumin Yuan Ya-Min Li Zhuhui Wang Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation Drug Delivery extracellular vesicles exosomes preservation storage nanomedicine |
| title | Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation |
| title_full | Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation |
| title_fullStr | Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation |
| title_full_unstemmed | Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation |
| title_short | Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation |
| title_sort | preserving extracellular vesicles for biomedical applications consideration of storage stability before and after isolation |
| topic | extracellular vesicles exosomes preservation storage nanomedicine |
| url | http://dx.doi.org/10.1080/10717544.2021.1951896 |
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