Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance
The 12-oxo-phytodienoic acid reductases (<i>OPRs</i>) have been proven to play a major role in plant development and growth. Although the classification and functions of <i>OPRs</i> have been well understood in Arabidopsis, tomato, rice, maize, and wheat, the information of &...
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MDPI AG
2020-09-01
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| author | Shichao Liu Ruibin Sun Xiaojian Zhang Zili Feng Feng Wei Lihong Zhao Yalin Zhang Longfu Zhu Hongjie Feng Heqin Zhu |
| author_facet | Shichao Liu Ruibin Sun Xiaojian Zhang Zili Feng Feng Wei Lihong Zhao Yalin Zhang Longfu Zhu Hongjie Feng Heqin Zhu |
| author_sort | Shichao Liu |
| collection | DOAJ |
| description | The 12-oxo-phytodienoic acid reductases (<i>OPRs</i>) have been proven to play a major role in plant development and growth. Although the classification and functions of <i>OPRs</i> have been well understood in Arabidopsis, tomato, rice, maize, and wheat, the information of <i>OPR</i> genes in cotton genome and their responses to biotic and abiotic stresses have not been reported. In this study, we found 10 and 9 <i>OPR</i> genes in <i>Gossypium hirsutum</i> and <i>Gossypium barbadense</i>, respectively. They were classified into three groups, based on the similar gene structure and conserved protein motifs. These <i>OPR</i> genes just located on chromosome 01, chromosome 05, and chromosome 06. In addition, the whole genome duplication (WGD) or segmental duplication events contributed to the evolution of the <i>OPR</i> gene family. The analyses of cis-acting regulatory elements of <i>GhOPRs</i> showed that the functions of <i>OPR</i> genes in cotton might be related to growth, development, hormone, and stresses. Expression patterns showed that <i>GhOPRs</i> were upregulated under salt treatment and repressed by polyethylene glycol 6000 (PEG6000). The expression patterns of <i>GhOPRs</i> were different in leaf, root, and stem under <i>V. dahliae</i> infection. <i>GhOPR9</i> showed a higher expression level than other <i>OPR</i> genes in cotton root. The virus-induced gene silencing (VIGS) analysis suggested that knockdown of <i>GhOPR9</i> could increase the susceptibility of cotton to <i>V. dahliae</i> infection. Furthermore, <i>GhOPR9</i> also modulated the expressions of jasmonic acid (JA) pathway-regulated genes under the <i>V. dahliae</i> infection. Overall, our results provided the evolution and potential functions of the <i>OPR</i> genes in cotton. These findings suggested that <i>GhOPR9</i> might play an important role in cotton resistance to <i>V. dahliae</i>. |
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| spelling | doaj.art-f0427192bdf84b178ee57a4e57387a032023-11-20T15:17:18ZengMDPI AGGenes2073-44252020-09-011110113410.3390/genes11101134Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> ResistanceShichao Liu0Ruibin Sun1Xiaojian Zhang2Zili Feng3Feng Wei4Lihong Zhao5Yalin Zhang6Longfu Zhu7Hongjie Feng8Heqin Zhu9State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaThe 12-oxo-phytodienoic acid reductases (<i>OPRs</i>) have been proven to play a major role in plant development and growth. Although the classification and functions of <i>OPRs</i> have been well understood in Arabidopsis, tomato, rice, maize, and wheat, the information of <i>OPR</i> genes in cotton genome and their responses to biotic and abiotic stresses have not been reported. In this study, we found 10 and 9 <i>OPR</i> genes in <i>Gossypium hirsutum</i> and <i>Gossypium barbadense</i>, respectively. They were classified into three groups, based on the similar gene structure and conserved protein motifs. These <i>OPR</i> genes just located on chromosome 01, chromosome 05, and chromosome 06. In addition, the whole genome duplication (WGD) or segmental duplication events contributed to the evolution of the <i>OPR</i> gene family. The analyses of cis-acting regulatory elements of <i>GhOPRs</i> showed that the functions of <i>OPR</i> genes in cotton might be related to growth, development, hormone, and stresses. Expression patterns showed that <i>GhOPRs</i> were upregulated under salt treatment and repressed by polyethylene glycol 6000 (PEG6000). The expression patterns of <i>GhOPRs</i> were different in leaf, root, and stem under <i>V. dahliae</i> infection. <i>GhOPR9</i> showed a higher expression level than other <i>OPR</i> genes in cotton root. The virus-induced gene silencing (VIGS) analysis suggested that knockdown of <i>GhOPR9</i> could increase the susceptibility of cotton to <i>V. dahliae</i> infection. Furthermore, <i>GhOPR9</i> also modulated the expressions of jasmonic acid (JA) pathway-regulated genes under the <i>V. dahliae</i> infection. Overall, our results provided the evolution and potential functions of the <i>OPR</i> genes in cotton. These findings suggested that <i>GhOPR9</i> might play an important role in cotton resistance to <i>V. dahliae</i>.https://www.mdpi.com/2073-4425/11/10/1134OPRcottongene family<i>Verticillium dahliae</i>VIGS |
| spellingShingle | Shichao Liu Ruibin Sun Xiaojian Zhang Zili Feng Feng Wei Lihong Zhao Yalin Zhang Longfu Zhu Hongjie Feng Heqin Zhu Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance Genes OPR cotton gene family <i>Verticillium dahliae</i> VIGS |
| title | Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance |
| title_full | Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance |
| title_fullStr | Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance |
| title_full_unstemmed | Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance |
| title_short | Genome-Wide Analysis of <i>OPR</i> Family Genes in Cotton Identified a Role for <i>GhOPR9</i> in <i>Verticillium dahliae</i> Resistance |
| title_sort | genome wide analysis of i opr i family genes in cotton identified a role for i ghopr9 i in i verticillium dahliae i resistance |
| topic | OPR cotton gene family <i>Verticillium dahliae</i> VIGS |
| url | https://www.mdpi.com/2073-4425/11/10/1134 |
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