Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice
Background: Given the importance of environmental factors and the impact of environmental pollutants, such as dioxins, on organ systems, especially the reproductive system, it is necessary to study these issues and the mechanisms of their effects. Objectives: This study aimed to investigate the eff...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2023-12-01
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| Series: | Pharmaceutical and Biomedical Research |
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| Online Access: | http://pbr.mazums.ac.ir/article-1-325-en.html |
| _version_ | 1811155650051309568 |
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| author | Shabnam Mohammadi Narjes Jalilvand Fatemeh Esfandiari Seyed Saleh Attari |
| author_facet | Shabnam Mohammadi Narjes Jalilvand Fatemeh Esfandiari Seyed Saleh Attari |
| author_sort | Shabnam Mohammadi |
| collection | DOAJ |
| description | Background: Given the importance of environmental factors and the impact of environmental pollutants, such as dioxins, on organ systems, especially the reproductive system, it is necessary to study these issues and the mechanisms of their effects.
Objectives: This study aimed to investigate the effects of different doses of dioxins on DNA damage in the testes of adult male mice.
Methods: In this experimental study, 32 male Naval Medical Research Institute (NMRI) rats were randomly divided into four groups: The control group received normal saline, and the dioxin groups were treated with different doses (0.1, 0.5, and 1 µg/kg) for two weeks. Apoptosis in the testes was then examined using a TUNEL assay kit.
Results: The mean number of TUNEL-positive spermatogonia cells was 5.91±5.28 in the dioxin group 1, 7.20±10.03 in the dioxin group 2, and 8.73±4.63 in the dioxin group 3, which was higher than that in the control group (0.16±0.40; P=0.073, P=0.034, and P=0.007, respectively).
The mean number of TUNEL-positive spermatocyte cells was 5.16±1.99 in the dioxin group 1, 2.50±4.62 in the dioxin group 2, and 3.33±2.94 in the dioxin group 3, which was higher than that in the control group (P=0.034, P=0.14, and P=0.037, respectively).
The mean number of TUNEL-positive spermatocyte cells in the dioxin group 1 was significantly higher than that in the dioxin group 2 (2.50±4.62, P=0.047).
The average number of TUNEL-positive spermatid cells was 11.58±6.90 in the dioxin group 1, 11.10±12.19 in the dioxin group 2, and 10.20±7.32 in the 3-dioxin group, which was higher than that in the control group (0.16±0.40; P=0.008, P=0.014, and P=0.015, respectively).
Conclusion: The results of the present study showed that dioxin caused dose-dependent apoptosis in the testes. |
| first_indexed | 2024-04-10T04:38:05Z |
| format | Article |
| id | doaj.art-f048aa38f3ac4022a326b220892a988e |
| institution | Directory Open Access Journal |
| issn | 2423-4494 |
| language | English |
| last_indexed | 2024-04-10T04:38:05Z |
| publishDate | 2023-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical and Biomedical Research |
| spelling | doaj.art-f048aa38f3ac4022a326b220892a988e2023-03-09T18:08:42ZengMazandaran University of Medical SciencesPharmaceutical and Biomedical Research2423-44942023-12-019116Effects of Dioxin on DNA Damage in the Testes of Adult Male MiceShabnam Mohammadi0Narjes Jalilvand1Fatemeh Esfandiari2Seyed Saleh Attari3 Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Background: Given the importance of environmental factors and the impact of environmental pollutants, such as dioxins, on organ systems, especially the reproductive system, it is necessary to study these issues and the mechanisms of their effects. Objectives: This study aimed to investigate the effects of different doses of dioxins on DNA damage in the testes of adult male mice. Methods: In this experimental study, 32 male Naval Medical Research Institute (NMRI) rats were randomly divided into four groups: The control group received normal saline, and the dioxin groups were treated with different doses (0.1, 0.5, and 1 µg/kg) for two weeks. Apoptosis in the testes was then examined using a TUNEL assay kit. Results: The mean number of TUNEL-positive spermatogonia cells was 5.91±5.28 in the dioxin group 1, 7.20±10.03 in the dioxin group 2, and 8.73±4.63 in the dioxin group 3, which was higher than that in the control group (0.16±0.40; P=0.073, P=0.034, and P=0.007, respectively). The mean number of TUNEL-positive spermatocyte cells was 5.16±1.99 in the dioxin group 1, 2.50±4.62 in the dioxin group 2, and 3.33±2.94 in the dioxin group 3, which was higher than that in the control group (P=0.034, P=0.14, and P=0.037, respectively). The mean number of TUNEL-positive spermatocyte cells in the dioxin group 1 was significantly higher than that in the dioxin group 2 (2.50±4.62, P=0.047). The average number of TUNEL-positive spermatid cells was 11.58±6.90 in the dioxin group 1, 11.10±12.19 in the dioxin group 2, and 10.20±7.32 in the 3-dioxin group, which was higher than that in the control group (0.16±0.40; P=0.008, P=0.014, and P=0.015, respectively). Conclusion: The results of the present study showed that dioxin caused dose-dependent apoptosis in the testes.http://pbr.mazums.ac.ir/article-1-325-en.htmlapoptosistestisdioxinsmicespermatozoatoxicity |
| spellingShingle | Shabnam Mohammadi Narjes Jalilvand Fatemeh Esfandiari Seyed Saleh Attari Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice Pharmaceutical and Biomedical Research apoptosis testis dioxins mice spermatozoa toxicity |
| title | Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice |
| title_full | Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice |
| title_fullStr | Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice |
| title_full_unstemmed | Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice |
| title_short | Effects of Dioxin on DNA Damage in the Testes of Adult Male Mice |
| title_sort | effects of dioxin on dna damage in the testes of adult male mice |
| topic | apoptosis testis dioxins mice spermatozoa toxicity |
| url | http://pbr.mazums.ac.ir/article-1-325-en.html |
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