<b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest
The induction of nodular culture (NC) and the subsequent development of microshoots of V. reitzii are considered an in vitro propagation model-system with high regenerative performance. Current research analyzed the determinant factors of the in vitro morphogenesis control of bromeliads. Seeds excis...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2014-05-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/21006 |
| _version_ | 1818531462601768960 |
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| author | Lírio Luiz Dal Vesco Rosete Pescador Jenny Paola Corredor Prado Leocir José Welter Miguel Pedro Guerra |
| author_facet | Lírio Luiz Dal Vesco Rosete Pescador Jenny Paola Corredor Prado Leocir José Welter Miguel Pedro Guerra |
| author_sort | Lírio Luiz Dal Vesco |
| collection | DOAJ |
| description | The induction of nodular culture (NC) and the subsequent development of microshoots of V. reitzii are considered an in vitro propagation model-system with high regenerative performance. Current research analyzed the determinant factors of the in vitro morphogenesis control of bromeliads. Seeds excised from mature capsules were grown on medium MS basic (MSB), liquid or gelled, supplemented or not with α-naphthaleneacetic acid (NAA), 6-benzilaminopurine (BAP) or thidiazuron (TDZ). The regeneration and elongation of microshoots were evaluated from NC sub-cultivated on MSB medium on liquid culture medium supplemented with different concentrations of indolyl-3-acetic acid (IAA) and gibberellic acid (GA3). Plant growth regulators (PGR) supplemented into the medium MSB inhibited the germination of the seeds and induced NC in the second week of growth. The induced NC on MSB medium with NAA (4 µM) and sub-cultivated on MSB medium with NAA (2 µM) plus N6(2-isopentenyl) adenine (2-iP) (2 µM) showed granular texture and high rate of proliferation. NC sub-culture in MSB medium with IAA (4 µM) provided a higher average number of microshoots (1,478 shoots g-1 of NC). Shoots over 3.0 cm resulted in more than 95% ex vitro survival. |
| first_indexed | 2024-12-11T17:32:47Z |
| format | Article |
| id | doaj.art-f04a874a3f724a0bb5cde4a1420f102a |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-11T17:32:47Z |
| publishDate | 2014-05-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-f04a874a3f724a0bb5cde4a1420f102a2022-12-22T00:56:46ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2014-05-0136327127810.4025/actascibiolsci.v36i3.2100611137<b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforestLírio Luiz Dal Vesco0Rosete Pescador1Jenny Paola Corredor Prado2Leocir José Welter3Miguel Pedro Guerra4Universidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaThe induction of nodular culture (NC) and the subsequent development of microshoots of V. reitzii are considered an in vitro propagation model-system with high regenerative performance. Current research analyzed the determinant factors of the in vitro morphogenesis control of bromeliads. Seeds excised from mature capsules were grown on medium MS basic (MSB), liquid or gelled, supplemented or not with α-naphthaleneacetic acid (NAA), 6-benzilaminopurine (BAP) or thidiazuron (TDZ). The regeneration and elongation of microshoots were evaluated from NC sub-cultivated on MSB medium on liquid culture medium supplemented with different concentrations of indolyl-3-acetic acid (IAA) and gibberellic acid (GA3). Plant growth regulators (PGR) supplemented into the medium MSB inhibited the germination of the seeds and induced NC in the second week of growth. The induced NC on MSB medium with NAA (4 µM) and sub-cultivated on MSB medium with NAA (2 µM) plus N6(2-isopentenyl) adenine (2-iP) (2 µM) showed granular texture and high rate of proliferation. NC sub-culture in MSB medium with IAA (4 µM) provided a higher average number of microshoots (1,478 shoots g-1 of NC). Shoots over 3.0 cm resulted in more than 95% ex vitro survival.http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/21006adventitious shootsconservationnodular cultureregeneration |
| spellingShingle | Lírio Luiz Dal Vesco Rosete Pescador Jenny Paola Corredor Prado Leocir José Welter Miguel Pedro Guerra <b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest Acta Scientiarum: Biological Sciences adventitious shoots conservation nodular culture regeneration |
| title | <b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest |
| title_full | <b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest |
| title_fullStr | <b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest |
| title_full_unstemmed | <b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest |
| title_short | <b><i>In vitro</i> propagation of <i>Vriesea reitzii</i>, a native epiphyte bromeliad from the Atlantic rainforest |
| title_sort | b i in vitro i propagation of i vriesea reitzii i a native epiphyte bromeliad from the atlantic rainforest |
| topic | adventitious shoots conservation nodular culture regeneration |
| url | http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/21006 |
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