Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice
Social stress, including bullying during adolescence, is a risk factor for common psychopathologies such as depression. To investigate the neural mechanisms associated with juvenile social stress-induced mood-related endophenotypes, we examined the behavioral, morphological, and biochemical effects...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-12-01
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| Series: | Neurobiology of Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352289516300108 |
| _version_ | 1819170525467901952 |
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| author | Sergio D. Iñiguez Antonio Aubry Lace M. Riggs Jason B. Alipio Roseanna M. Zanca Francisco J. Flores-Ramirez Mirella A. Hernandez Steven J. Nieto David Musheyev Peter A. Serrano |
| author_facet | Sergio D. Iñiguez Antonio Aubry Lace M. Riggs Jason B. Alipio Roseanna M. Zanca Francisco J. Flores-Ramirez Mirella A. Hernandez Steven J. Nieto David Musheyev Peter A. Serrano |
| author_sort | Sergio D. Iñiguez |
| collection | DOAJ |
| description | Social stress, including bullying during adolescence, is a risk factor for common psychopathologies such as depression. To investigate the neural mechanisms associated with juvenile social stress-induced mood-related endophenotypes, we examined the behavioral, morphological, and biochemical effects of the social defeat stress model of depression on hippocampal dendritic spines within the CA1 stratum radiatum. Adolescent (postnatal day 35) male C57BL/6 mice were subjected to defeat episodes for 10 consecutive days. Twenty-four h later, separate groups of mice were tested on the social interaction and tail suspension tests.
Hippocampi were then dissected and Western blots were conducted to quantify protein levels for various markers important for synaptic plasticity including protein kinase M zeta (PKMζ), protein kinase C zeta (PKCζ), the dopamine-1 (D1) receptor, tyrosine hydroxylase (TH), and the dopamine transporter (DAT). Furthermore, we examined the presence of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-receptor subunit GluA2 as well as colocalization with the post-synaptic density 95 (PSD95) protein, within different spine subtypes (filopodia, stubby, long-thin, mushroom) using an immunohistochemistry and Golgi-Cox staining technique. The results revealed that social defeat induced a depression-like behavioral profile, as inferred from decreased social interaction levels, increased immobility on the tail suspension test, and decreases in body weight. Whole hippocampal immunoblots revealed decreases in GluA2, with a concomitant increase in DAT and TH levels in the stressed group. Spine morphology analyses further showed that defeated mice displayed a significant decrease in stubby spines, and an increase in long-thin spines within the CA1 stratum radiatum. Further evaluation of GluA2/PSD95 containing-spines demonstrated a decrease of these markers within long-thin and mushroom spine types. Together, these results indicate that juvenile social stress induces GluA2- and dopamine-associated dysregulation in the hippocampus – a neurobiological mechanism potentially underlying the development of mood-related syndromes as a consequence of adolescent bullying. |
| first_indexed | 2024-12-22T19:36:47Z |
| format | Article |
| id | doaj.art-f04ac3ab547e43f5835a51269f7c0aa1 |
| institution | Directory Open Access Journal |
| issn | 2352-2895 |
| language | English |
| last_indexed | 2024-12-22T19:36:47Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Stress |
| spelling | doaj.art-f04ac3ab547e43f5835a51269f7c0aa12022-12-21T18:14:57ZengElsevierNeurobiology of Stress2352-28952016-12-015C546410.1016/j.ynstr.2016.07.001Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 miceSergio D. Iñiguez0Antonio Aubry1Lace M. Riggs2Jason B. Alipio3Roseanna M. Zanca4Francisco J. Flores-Ramirez5Mirella A. Hernandez6Steven J. Nieto7David Musheyev8Peter A. Serrano9Department of Psychology, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX, 79902, USADepartment of Psychology, Hunter College, New York, NY, 10065, USADepartment of Psychology, California State University, San Bernardino, CA, 92407, USADepartment of Psychology, California State University, San Bernardino, CA, 92407, USADepartment of Psychology, Hunter College, New York, NY, 10065, USADepartment of Psychology, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX, 79902, USADepartment of Psychology, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX, 79902, USADepartment of Psychology, California State University, San Bernardino, CA, 92407, USADepartment of Psychology, Hunter College, New York, NY, 10065, USADepartment of Psychology, Hunter College, New York, NY, 10065, USASocial stress, including bullying during adolescence, is a risk factor for common psychopathologies such as depression. To investigate the neural mechanisms associated with juvenile social stress-induced mood-related endophenotypes, we examined the behavioral, morphological, and biochemical effects of the social defeat stress model of depression on hippocampal dendritic spines within the CA1 stratum radiatum. Adolescent (postnatal day 35) male C57BL/6 mice were subjected to defeat episodes for 10 consecutive days. Twenty-four h later, separate groups of mice were tested on the social interaction and tail suspension tests. Hippocampi were then dissected and Western blots were conducted to quantify protein levels for various markers important for synaptic plasticity including protein kinase M zeta (PKMζ), protein kinase C zeta (PKCζ), the dopamine-1 (D1) receptor, tyrosine hydroxylase (TH), and the dopamine transporter (DAT). Furthermore, we examined the presence of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-receptor subunit GluA2 as well as colocalization with the post-synaptic density 95 (PSD95) protein, within different spine subtypes (filopodia, stubby, long-thin, mushroom) using an immunohistochemistry and Golgi-Cox staining technique. The results revealed that social defeat induced a depression-like behavioral profile, as inferred from decreased social interaction levels, increased immobility on the tail suspension test, and decreases in body weight. Whole hippocampal immunoblots revealed decreases in GluA2, with a concomitant increase in DAT and TH levels in the stressed group. Spine morphology analyses further showed that defeated mice displayed a significant decrease in stubby spines, and an increase in long-thin spines within the CA1 stratum radiatum. Further evaluation of GluA2/PSD95 containing-spines demonstrated a decrease of these markers within long-thin and mushroom spine types. Together, these results indicate that juvenile social stress induces GluA2- and dopamine-associated dysregulation in the hippocampus – a neurobiological mechanism potentially underlying the development of mood-related syndromes as a consequence of adolescent bullying.http://www.sciencedirect.com/science/article/pii/S2352289516300108BullyingCA1DepressionDopamineGluA2JuvenileTail suspension test |
| spellingShingle | Sergio D. Iñiguez Antonio Aubry Lace M. Riggs Jason B. Alipio Roseanna M. Zanca Francisco J. Flores-Ramirez Mirella A. Hernandez Steven J. Nieto David Musheyev Peter A. Serrano Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice Neurobiology of Stress Bullying CA1 Depression Dopamine GluA2 Juvenile Tail suspension test |
| title | Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice |
| title_full | Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice |
| title_fullStr | Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice |
| title_full_unstemmed | Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice |
| title_short | Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice |
| title_sort | social defeat stress induces depression like behavior and alters spine morphology in the hippocampus of adolescent male c57bl 6 mice |
| topic | Bullying CA1 Depression Dopamine GluA2 Juvenile Tail suspension test |
| url | http://www.sciencedirect.com/science/article/pii/S2352289516300108 |
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