Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle
Motor endplates (MEPs) are important sites of information exchange between motor neurons and skeletal muscle, and are distributed in an organized pattern of lamellae in the muscle. Delayed repair of peripheral nerve injury typically results in unsatisfactory functional recovery because of MEP degene...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=2;spage=459;epage=464;aulast=Li |
| _version_ | 1818906148917477376 |
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| author | Dong-Dong Li Jin Deng Bo Jin Shuai Han Xin-Yi Gu Xue-Feng Zhou Xiao-Feng Yin |
| author_facet | Dong-Dong Li Jin Deng Bo Jin Shuai Han Xin-Yi Gu Xue-Feng Zhou Xiao-Feng Yin |
| author_sort | Dong-Dong Li |
| collection | DOAJ |
| description | Motor endplates (MEPs) are important sites of information exchange between motor neurons and skeletal muscle, and are distributed in an organized pattern of lamellae in the muscle. Delayed repair of peripheral nerve injury typically results in unsatisfactory functional recovery because of MEP degeneration. In this study, the mouse tibial nerve was transected and repaired with a biodegradable chitin conduit, immediately following or 1 or 3 months after the injury. Fluorescent α-bungarotoxin was injected to label MEPs. Tissue optical clearing combined with light-sheet microscopy revealed that MEPs were distributed in an organized pattern of lamellae in skeletal muscle after delayed repair for 1 and 3 months. However, the total number of MEPs, the number of MEPs per lamellar cluster, and the maturation of single MEPs in gastrocnemius muscle gradually decreased with increasing denervation time. These findings suggest that delayed repair can restore the spatial distribution of MEPs, but it has an adverse effect on the homogeneity of MEPs in the lamellar clusters and the total number of MEPs in the target muscle. The study procedures were approved by the Animal Ethics Committee of the Peking University People’s Hospital (approval No. 2019PHC015) on April 8, 2019. |
| first_indexed | 2024-12-19T21:34:38Z |
| format | Article |
| id | doaj.art-f04d8c94172b4cd1beee3ed7703d143e |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-19T21:34:38Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-f04d8c94172b4cd1beee3ed7703d143e2022-12-21T20:04:50ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117245946410.4103/1673-5374.317990Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscleDong-Dong LiJin DengBo JinShuai HanXin-Yi GuXue-Feng ZhouXiao-Feng YinMotor endplates (MEPs) are important sites of information exchange between motor neurons and skeletal muscle, and are distributed in an organized pattern of lamellae in the muscle. Delayed repair of peripheral nerve injury typically results in unsatisfactory functional recovery because of MEP degeneration. In this study, the mouse tibial nerve was transected and repaired with a biodegradable chitin conduit, immediately following or 1 or 3 months after the injury. Fluorescent α-bungarotoxin was injected to label MEPs. Tissue optical clearing combined with light-sheet microscopy revealed that MEPs were distributed in an organized pattern of lamellae in skeletal muscle after delayed repair for 1 and 3 months. However, the total number of MEPs, the number of MEPs per lamellar cluster, and the maturation of single MEPs in gastrocnemius muscle gradually decreased with increasing denervation time. These findings suggest that delayed repair can restore the spatial distribution of MEPs, but it has an adverse effect on the homogeneity of MEPs in the lamellar clusters and the total number of MEPs in the target muscle. The study procedures were approved by the Animal Ethics Committee of the Peking University People’s Hospital (approval No. 2019PHC015) on April 8, 2019.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=2;spage=459;epage=464;aulast=Lidegeneration; delayed repair; lamellar cluster; light-sheet microscopy; motor endplates; peripheral nerve injury; three-dimensional distribution; tissue optical clearing |
| spellingShingle | Dong-Dong Li Jin Deng Bo Jin Shuai Han Xin-Yi Gu Xue-Feng Zhou Xiao-Feng Yin Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle Neural Regeneration Research degeneration; delayed repair; lamellar cluster; light-sheet microscopy; motor endplates; peripheral nerve injury; three-dimensional distribution; tissue optical clearing |
| title | Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle |
| title_full | Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle |
| title_fullStr | Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle |
| title_full_unstemmed | Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle |
| title_short | Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle |
| title_sort | effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle |
| topic | degeneration; delayed repair; lamellar cluster; light-sheet microscopy; motor endplates; peripheral nerve injury; three-dimensional distribution; tissue optical clearing |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=2;spage=459;epage=464;aulast=Li |
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