Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance
The interaction of heat stress with internal signaling networks was investigated through Arabidopsis thaliana mutants that were deficient in either tocopherols (vte1 mutant) or non-photochemical fluorescence quenching (NPQ; npq1, npq4, and npq1 npq4 mutants). Leaves of both vte1 and npq1 npq4 mutant...
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2018-08-01
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author | Jared J. Stewart Christopher R. Baker Carlie S. Sharpes Shannon Toy Wong-Michalak Stephanie K. Polutchko William W. Adams Barbara Demmig-Adams |
author_facet | Jared J. Stewart Christopher R. Baker Carlie S. Sharpes Shannon Toy Wong-Michalak Stephanie K. Polutchko William W. Adams Barbara Demmig-Adams |
author_sort | Jared J. Stewart |
collection | DOAJ |
description | The interaction of heat stress with internal signaling networks was investigated through Arabidopsis thaliana mutants that were deficient in either tocopherols (vte1 mutant) or non-photochemical fluorescence quenching (NPQ; npq1, npq4, and npq1 npq4 mutants). Leaves of both vte1 and npq1 npq4 mutants that developed at a high temperature exhibited a significantly different leaf vascular organization compared to wild-type Col-0. Both mutants had significantly smaller water conduits (tracheary elements) of the xylem, but the total apparent foliar water-transport capacity and intrinsic photosynthetic capacity were similarly high in mutants and wild-type Col-0. This was accomplished through a combination of more numerous (albeit narrower) water conduits per vein, and a significantly greater vein density in both mutants relative to wild-type Col-0. The similarity of the phenotypes of tocopherol-deficient and NPQ-deficient mutants suggests that leaf vasculature organization is modulated by the foliar redox state. These results are evaluated in the context of interactions between redox-signaling pathways and other key regulators of plant acclimation to growth temperature, such as the C-repeat binding factor (CBF) transcription factors, several of which were upregulated in the antioxidant-deficient mutants. Possibilities for the future manipulation of the interaction between CBF and redox-signaling networks for the purpose of cooptimizing plant productivity and plant tolerance to extreme temperatures are discussed. |
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language | English |
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spelling | doaj.art-f04db56312604cf7960d342d5a434ee42022-12-22T03:08:11ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-08-01199250710.3390/ijms19092507ijms19092507Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat ToleranceJared J. Stewart0Christopher R. Baker1Carlie S. Sharpes2Shannon Toy Wong-Michalak3Stephanie K. Polutchko4William W. Adams5Barbara Demmig-Adams6Department of Ecology & Evolutionary Biology, University of Colorado, Boulder, CO 80309-0334, USADepartment of Plant & Microbial Biology, University of California, Berkeley, CA 94720-3102, USADepartment of Ecology & Evolutionary Biology, University of Colorado, Boulder, CO 80309-0334, USADepartment of Plant & Microbial Biology, University of California, Berkeley, CA 94720-3102, USADepartment of Ecology & Evolutionary Biology, University of Colorado, Boulder, CO 80309-0334, USADepartment of Ecology & Evolutionary Biology, University of Colorado, Boulder, CO 80309-0334, USADepartment of Ecology & Evolutionary Biology, University of Colorado, Boulder, CO 80309-0334, USAThe interaction of heat stress with internal signaling networks was investigated through Arabidopsis thaliana mutants that were deficient in either tocopherols (vte1 mutant) or non-photochemical fluorescence quenching (NPQ; npq1, npq4, and npq1 npq4 mutants). Leaves of both vte1 and npq1 npq4 mutants that developed at a high temperature exhibited a significantly different leaf vascular organization compared to wild-type Col-0. Both mutants had significantly smaller water conduits (tracheary elements) of the xylem, but the total apparent foliar water-transport capacity and intrinsic photosynthetic capacity were similarly high in mutants and wild-type Col-0. This was accomplished through a combination of more numerous (albeit narrower) water conduits per vein, and a significantly greater vein density in both mutants relative to wild-type Col-0. The similarity of the phenotypes of tocopherol-deficient and NPQ-deficient mutants suggests that leaf vasculature organization is modulated by the foliar redox state. These results are evaluated in the context of interactions between redox-signaling pathways and other key regulators of plant acclimation to growth temperature, such as the C-repeat binding factor (CBF) transcription factors, several of which were upregulated in the antioxidant-deficient mutants. Possibilities for the future manipulation of the interaction between CBF and redox-signaling networks for the purpose of cooptimizing plant productivity and plant tolerance to extreme temperatures are discussed.http://www.mdpi.com/1422-0067/19/9/2507ArabidopsisantioxidantC-repeat binding factorphloemphotoprotectionphotosynthesisPsbStocopherolxylemzeaxanthin |
spellingShingle | Jared J. Stewart Christopher R. Baker Carlie S. Sharpes Shannon Toy Wong-Michalak Stephanie K. Polutchko William W. Adams Barbara Demmig-Adams Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance International Journal of Molecular Sciences Arabidopsis antioxidant C-repeat binding factor phloem photoprotection photosynthesis PsbS tocopherol xylem zeaxanthin |
title | Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance |
title_full | Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance |
title_fullStr | Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance |
title_full_unstemmed | Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance |
title_short | Effects of Foliar Redox Status on Leaf Vascular Organization Suggest Avenues for Cooptimization of Photosynthesis and Heat Tolerance |
title_sort | effects of foliar redox status on leaf vascular organization suggest avenues for cooptimization of photosynthesis and heat tolerance |
topic | Arabidopsis antioxidant C-repeat binding factor phloem photoprotection photosynthesis PsbS tocopherol xylem zeaxanthin |
url | http://www.mdpi.com/1422-0067/19/9/2507 |
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