Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells
The study was designed to evaluate reactive oxygen species (ROS)/nitric oxide (NO) formation and apoptotic/necrotic cell death elicited by prednisolone in peripheral blood and bone marrow mononuclear cells and to define the efficacy of vitamin D3 to counter glucocorticoid (GC)-induced changes. It wa...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2016-10-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2016/11/Shymanskyy_5_16.pdf |
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| author | I. O. Shymanskyy O. O. Lisakovska A. O. Mazanova, D. O. Labudzynskyi A. V. Khomenko, M. M. Veliky |
| author_facet | I. O. Shymanskyy O. O. Lisakovska A. O. Mazanova, D. O. Labudzynskyi A. V. Khomenko, M. M. Veliky |
| author_sort | I. O. Shymanskyy |
| collection | DOAJ |
| description | The study was designed to evaluate reactive oxygen species (ROS)/nitric oxide (NO) formation and apoptotic/necrotic cell death elicited by prednisolone in peripheral blood and bone marrow mononuclear cells and to define the efficacy of vitamin D3 to counter glucocorticoid (GC)-induced changes. It was shown that prednisolone (5 mg per kg of female Wistar rat’s body weight for 30 days) evoked ROS and NO overproduction by blood mononuclear cells (monocytes and lymphocytes) that correlated with increased cell apoptosis and necrosis. In contrast, prednisolone did not affect ROS/NO levels in bone marrow mononuclear cells that corresponded to lower level of cell death than in the control. Alterations of prooxidant processes revealed in mononuclear cells and associated with GC action were accompanied by vitamin D3 deficiency in animals, which was assessed by the decreased level of blood serum 25-hydroxivitamin D3 (25OHD3). Vitamin D3 administration (100 IU per rat daily for 30 days, concurrently with prednisolone administration) completely restored 25OHD3 content to the control values and significantly reversed ROS and NO formation in blood mononuclear cells, thus leading to decreased apoptosis. In bone marrow, vitamin D3 activated ROS/NO production and protein nitration that may play a role in prevention of prednisolone-elicited increase in bone resorption. We conclude that vitamin D3 shows a profound protection against GC-associated cellular damage through regulating intracellular ROS/NO formation and cell death pathways. |
| first_indexed | 2024-03-11T20:35:48Z |
| format | Article |
| id | doaj.art-f050f4d6a89a4a1f9fe0fea23f9d1bd4 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T20:35:48Z |
| publishDate | 2016-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-f050f4d6a89a4a1f9fe0fea23f9d1bd42023-10-02T07:15:50ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032016-10-01885384710.15407/ubj88.05.038Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cellsI. O. ShymanskyyO. O. LisakovskaA. O. Mazanova,D. O. Labudzynskyi A. V. Khomenko, M. M. VelikyThe study was designed to evaluate reactive oxygen species (ROS)/nitric oxide (NO) formation and apoptotic/necrotic cell death elicited by prednisolone in peripheral blood and bone marrow mononuclear cells and to define the efficacy of vitamin D3 to counter glucocorticoid (GC)-induced changes. It was shown that prednisolone (5 mg per kg of female Wistar rat’s body weight for 30 days) evoked ROS and NO overproduction by blood mononuclear cells (monocytes and lymphocytes) that correlated with increased cell apoptosis and necrosis. In contrast, prednisolone did not affect ROS/NO levels in bone marrow mononuclear cells that corresponded to lower level of cell death than in the control. Alterations of prooxidant processes revealed in mononuclear cells and associated with GC action were accompanied by vitamin D3 deficiency in animals, which was assessed by the decreased level of blood serum 25-hydroxivitamin D3 (25OHD3). Vitamin D3 administration (100 IU per rat daily for 30 days, concurrently with prednisolone administration) completely restored 25OHD3 content to the control values and significantly reversed ROS and NO formation in blood mononuclear cells, thus leading to decreased apoptosis. In bone marrow, vitamin D3 activated ROS/NO production and protein nitration that may play a role in prevention of prednisolone-elicited increase in bone resorption. We conclude that vitamin D3 shows a profound protection against GC-associated cellular damage through regulating intracellular ROS/NO formation and cell death pathways.http://ukrbiochemjournal.org/wp-content/uploads/2016/11/Shymanskyy_5_16.pdfapoptosisblood/bone marrow mononuclear cellsnecrosisnitric oxideosteoporosisprednisolonereactive oxygen/nitrogen speciesvitamin D3 |
| spellingShingle | I. O. Shymanskyy O. O. Lisakovska A. O. Mazanova, D. O. Labudzynskyi A. V. Khomenko, M. M. Veliky Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells The Ukrainian Biochemical Journal apoptosis blood/bone marrow mononuclear cells necrosis nitric oxide osteoporosis prednisolone reactive oxygen/nitrogen species vitamin D3 |
| title | Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells |
| title_full | Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells |
| title_fullStr | Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells |
| title_full_unstemmed | Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells |
| title_short | Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells |
| title_sort | prednisolone and vitamin d 3 modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells |
| topic | apoptosis blood/bone marrow mononuclear cells necrosis nitric oxide osteoporosis prednisolone reactive oxygen/nitrogen species vitamin D3 |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2016/11/Shymanskyy_5_16.pdf |
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