The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2
Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is un...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.814929/full |
| _version_ | 1818954131576979456 |
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| author | Jian Liu Kaiquan Liu Zhe Zhao Zheng Wang Fengchao Wang Yuxiu Xin Jie Qu Feng Song Zhenghua Li |
| author_facet | Jian Liu Kaiquan Liu Zhe Zhao Zheng Wang Fengchao Wang Yuxiu Xin Jie Qu Feng Song Zhenghua Li |
| author_sort | Jian Liu |
| collection | DOAJ |
| description | Cyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Shewanella xiamenensis Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system. |
| first_indexed | 2024-12-20T10:17:17Z |
| format | Article |
| id | doaj.art-f05338b6985f4b8e88db9ca5d63a8cc7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T10:17:17Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f05338b6985f4b8e88db9ca5d63a8cc72022-12-21T19:44:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-01-011210.3389/fmicb.2021.814929814929The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2Jian Liu0Kaiquan Liu1Zhe Zhao2Zheng Wang3Fengchao Wang4Yuxiu Xin5Jie Qu6Feng Song7Zhenghua Li8Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaSchool of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaShandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, ChinaCyanobacterial blooming is an increasing environmental issue all over the world. Algicidal bacteria are potential tools for the control of algal blooms. The algicidal activity in many bacteria exhibits quorum-sensing (QS) dynamics and the regulatory mechanism of this activity in these bacteria is unclear. In this study, combining genomic sequencing and genome editing, we have identified that the primary quorum-sensing system in the isolated algicidal strain Shewanella xiamenensis Lzh-2 is the LuxS/AI-2 signaling pathway. Disruption of the QS system through recombination deletion of the LuxS gene led to a loss of algicides production and algicidal activity. Restoration of the LuxS gene in the deletion mutant compensated the QS system and recovered the algicidal activity. Consequently, we proved that Lzh-2 regulates the algicidal activity through LuxS/AI-2 quorum-sensing system.https://www.frontiersin.org/articles/10.3389/fmicb.2021.814929/fullquorum-sensingalgicidal bacteriaLuxS/AI-2Shewanella xiamenensisbloom control |
| spellingShingle | Jian Liu Kaiquan Liu Zhe Zhao Zheng Wang Fengchao Wang Yuxiu Xin Jie Qu Feng Song Zhenghua Li The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 Frontiers in Microbiology quorum-sensing algicidal bacteria LuxS/AI-2 Shewanella xiamenensis bloom control |
| title | The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 |
| title_full | The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 |
| title_fullStr | The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 |
| title_full_unstemmed | The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 |
| title_short | The LuxS/AI-2 Quorum-Sensing System Regulates the Algicidal Activity of Shewanella xiamenensis Lzh-2 |
| title_sort | luxs ai 2 quorum sensing system regulates the algicidal activity of shewanella xiamenensis lzh 2 |
| topic | quorum-sensing algicidal bacteria LuxS/AI-2 Shewanella xiamenensis bloom control |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.814929/full |
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