Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals?
Despite recent advances in methods for culturing Mycobacterium tuberculosis (MTB), the diagnostic yield of tuberculous pleural effusion (TBPE) remains unsatisfactory. However, unlike repeated sputum cultures of pulmonary tuberculosis, little is known about the role of repeated pleural cultures. We e...
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| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5531521?pdf=render |
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| author | Yousang Ko Jinkyung Song Suh-Young Lee Jin-Wook Moon Eun-Kyung Mo Ji Young Park Joo-Hee Kim Sunghoon Park Yong Il Hwang Seung Hun Jang Byung Woo Jhun Yun Su Sim Tae Rim Shin Dong-Gyu Kim Ji Young Hong Chang Youl Lee Myung Goo Lee Cheol-Hong Kim In Gyu Hyun Yong Bum Park |
| author_facet | Yousang Ko Jinkyung Song Suh-Young Lee Jin-Wook Moon Eun-Kyung Mo Ji Young Park Joo-Hee Kim Sunghoon Park Yong Il Hwang Seung Hun Jang Byung Woo Jhun Yun Su Sim Tae Rim Shin Dong-Gyu Kim Ji Young Hong Chang Youl Lee Myung Goo Lee Cheol-Hong Kim In Gyu Hyun Yong Bum Park |
| author_sort | Yousang Ko |
| collection | DOAJ |
| description | Despite recent advances in methods for culturing Mycobacterium tuberculosis (MTB), the diagnostic yield of tuberculous pleural effusion (TBPE) remains unsatisfactory. However, unlike repeated sputum cultures of pulmonary tuberculosis, little is known about the role of repeated pleural cultures. We examined whether repeated pleural cultures are associated with increased MTB yield from TBPE.A multicenter, retrospective cohort study was performed from January 2012 to December 2015 in South Korea. Patients were categorized into two groups: single- or repeated-culture groups. The diagnostic yield of MTB and clinical, radiological, and pleural fluid characteristics were evaluated.Among the 329 patients with TBPE, 77 (23.4%) had repeated cultures and 252 (76.5%) had a single culture. Pleural culture was performed twice in all 77 patients in the repeated-culture group at a 1-day interval (inter-quartile range, 1.0-2.0). In the repeated-culture group, the yield of MTB from the first culture was 31.2%, which was similar to that in the single-culture group (31.2% vs. 29.8%, P = 0.887). However, the yield of MTB from the second culture (10/77, 13.0%) was more than that from the first. These results may be attributable to the insufficient immune clearance for MTB invasion into the pleural space between the first and second cultures. Over time, the yield of the second cultures decreased from 17.4% to 6.7% and then 6.3%. Finally, the overall yield of MTB in the repeated- and single-culture groups was 44.2% and 29.8% respectively (P < 0.001).The results showed that repeated pleural cultures increased MTB yield from TBPE in human immunodeficiency virus-negative individuals. Furthermore, repeated cultures may increase yield when carried out for two consecutive days. |
| first_indexed | 2024-12-13T21:38:04Z |
| format | Article |
| id | doaj.art-f055568054e54c0e904589c906b6c860 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:38:04Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-f055568054e54c0e904589c906b6c8602022-12-21T23:30:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018179810.1371/journal.pone.0181798Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals?Yousang KoJinkyung SongSuh-Young LeeJin-Wook MoonEun-Kyung MoJi Young ParkJoo-Hee KimSunghoon ParkYong Il HwangSeung Hun JangByung Woo JhunYun Su SimTae Rim ShinDong-Gyu KimJi Young HongChang Youl LeeMyung Goo LeeCheol-Hong KimIn Gyu HyunYong Bum ParkDespite recent advances in methods for culturing Mycobacterium tuberculosis (MTB), the diagnostic yield of tuberculous pleural effusion (TBPE) remains unsatisfactory. However, unlike repeated sputum cultures of pulmonary tuberculosis, little is known about the role of repeated pleural cultures. We examined whether repeated pleural cultures are associated with increased MTB yield from TBPE.A multicenter, retrospective cohort study was performed from January 2012 to December 2015 in South Korea. Patients were categorized into two groups: single- or repeated-culture groups. The diagnostic yield of MTB and clinical, radiological, and pleural fluid characteristics were evaluated.Among the 329 patients with TBPE, 77 (23.4%) had repeated cultures and 252 (76.5%) had a single culture. Pleural culture was performed twice in all 77 patients in the repeated-culture group at a 1-day interval (inter-quartile range, 1.0-2.0). In the repeated-culture group, the yield of MTB from the first culture was 31.2%, which was similar to that in the single-culture group (31.2% vs. 29.8%, P = 0.887). However, the yield of MTB from the second culture (10/77, 13.0%) was more than that from the first. These results may be attributable to the insufficient immune clearance for MTB invasion into the pleural space between the first and second cultures. Over time, the yield of the second cultures decreased from 17.4% to 6.7% and then 6.3%. Finally, the overall yield of MTB in the repeated- and single-culture groups was 44.2% and 29.8% respectively (P < 0.001).The results showed that repeated pleural cultures increased MTB yield from TBPE in human immunodeficiency virus-negative individuals. Furthermore, repeated cultures may increase yield when carried out for two consecutive days.http://europepmc.org/articles/PMC5531521?pdf=render |
| spellingShingle | Yousang Ko Jinkyung Song Suh-Young Lee Jin-Wook Moon Eun-Kyung Mo Ji Young Park Joo-Hee Kim Sunghoon Park Yong Il Hwang Seung Hun Jang Byung Woo Jhun Yun Su Sim Tae Rim Shin Dong-Gyu Kim Ji Young Hong Chang Youl Lee Myung Goo Lee Cheol-Hong Kim In Gyu Hyun Yong Bum Park Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals? PLoS ONE |
| title | Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals? |
| title_full | Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals? |
| title_fullStr | Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals? |
| title_full_unstemmed | Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals? |
| title_short | Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals? |
| title_sort | does repeated pleural culture increase the diagnostic yield of mycobacterium tuberculosis from tuberculous pleural effusion in hiv negative individuals |
| url | http://europepmc.org/articles/PMC5531521?pdf=render |
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