A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire
<p>Abstract</p> <p>Background</p> <p>The phylogenetically conserved transcription factor Lola is essential for many aspects of axon growth and guidance, synapse formation and neural circuit development in <it>Drosophila</it>. To date it has been difficult, h...
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| Format: | Article |
| Language: | English |
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BMC
2011-11-01
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| Series: | Neural Development |
| Online Access: | http://www.neuraldevelopment.com/content/6/1/37 |
| _version_ | 1811259944610037760 |
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| author | Gates Michael A Kannan Ramakrishnan Giniger Edward |
| author_facet | Gates Michael A Kannan Ramakrishnan Giniger Edward |
| author_sort | Gates Michael A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The phylogenetically conserved transcription factor Lola is essential for many aspects of axon growth and guidance, synapse formation and neural circuit development in <it>Drosophila</it>. To date it has been difficult, however, to obtain an overall view of Lola functions and mechanisms.</p> <p>Results</p> <p>We use expression microarrays to identify the <it>lola</it>-dependent transcriptome in the <it>Drosophila </it>embryo. We find that <it>lola </it>regulates the expression of a large selection of genes that are known to affect each of several <it>lola</it>-dependent developmental processes. Among other loci, we find <it>lola </it>to be a negative regulator of <it>spire</it>, an actin nucleation factor that has been studied for its essential role in oogenesis. We show that <it>spire </it>is expressed in the nervous system and is required for a known <it>lola</it>-dependent axon guidance decision, growth of ISNb motor axons. We further show that reducing <it>spire </it>gene dosage suppresses this aspect of the <it>lola </it>phenotype, verifying that derepression of <it>spire </it>is an important contributor to the axon stalling phenotype of embryonic motor axons in <it>lola </it>mutants.</p> <p>Conclusions</p> <p>These data shed new light on the molecular mechanisms of many <it>lola</it>-dependent processes, and also identify several developmental processes not previously linked to <it>lola </it>that are apt to be regulated by this transcription factor. These data further demonstrate that excessive expression of the actin nucleation factor Spire is as deleterious for axon growth <it>in vivo </it>as is the loss of Spire, thus highlighting the need for a balance in the elementary steps of actin dynamics to achieve effective neuronal morphogenesis.</p> |
| first_indexed | 2024-04-12T18:39:06Z |
| format | Article |
| id | doaj.art-f056768a7b0a4e26971e0bf85d1e9f51 |
| institution | Directory Open Access Journal |
| issn | 1749-8104 |
| language | English |
| last_indexed | 2024-04-12T18:39:06Z |
| publishDate | 2011-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Neural Development |
| spelling | doaj.art-f056768a7b0a4e26971e0bf85d1e9f512022-12-22T03:20:50ZengBMCNeural Development1749-81042011-11-01613710.1186/1749-8104-6-37A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor SpireGates Michael AKannan RamakrishnanGiniger Edward<p>Abstract</p> <p>Background</p> <p>The phylogenetically conserved transcription factor Lola is essential for many aspects of axon growth and guidance, synapse formation and neural circuit development in <it>Drosophila</it>. To date it has been difficult, however, to obtain an overall view of Lola functions and mechanisms.</p> <p>Results</p> <p>We use expression microarrays to identify the <it>lola</it>-dependent transcriptome in the <it>Drosophila </it>embryo. We find that <it>lola </it>regulates the expression of a large selection of genes that are known to affect each of several <it>lola</it>-dependent developmental processes. Among other loci, we find <it>lola </it>to be a negative regulator of <it>spire</it>, an actin nucleation factor that has been studied for its essential role in oogenesis. We show that <it>spire </it>is expressed in the nervous system and is required for a known <it>lola</it>-dependent axon guidance decision, growth of ISNb motor axons. We further show that reducing <it>spire </it>gene dosage suppresses this aspect of the <it>lola </it>phenotype, verifying that derepression of <it>spire </it>is an important contributor to the axon stalling phenotype of embryonic motor axons in <it>lola </it>mutants.</p> <p>Conclusions</p> <p>These data shed new light on the molecular mechanisms of many <it>lola</it>-dependent processes, and also identify several developmental processes not previously linked to <it>lola </it>that are apt to be regulated by this transcription factor. These data further demonstrate that excessive expression of the actin nucleation factor Spire is as deleterious for axon growth <it>in vivo </it>as is the loss of Spire, thus highlighting the need for a balance in the elementary steps of actin dynamics to achieve effective neuronal morphogenesis.</p>http://www.neuraldevelopment.com/content/6/1/37 |
| spellingShingle | Gates Michael A Kannan Ramakrishnan Giniger Edward A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire Neural Development |
| title | A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire |
| title_full | A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire |
| title_fullStr | A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire |
| title_full_unstemmed | A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire |
| title_short | A genome-wide analysis reveals that the <it>Drosophila </it>transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire |
| title_sort | genome wide analysis reveals that the it drosophila it transcription factor lola promotes axon growth in part by suppressing expression of the actin nucleation factor spire |
| url | http://www.neuraldevelopment.com/content/6/1/37 |
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