Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction
Differences in producing performance and organoleptic meat characteristics among pig genotypes and/or producing types are widely known. These parameters are also subjected to the animal’s development, feeding and management. Detailed knowledge of the effects of production phase (PP), pig producing t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-06-01
|
| Series: | Animal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731121000628 |
| _version_ | 1818589096561344512 |
|---|---|
| author | L. Sarri J. Balcells G. de la Fuente M. Tor J. Gómez-Arrue A.R. Seradj |
| author_facet | L. Sarri J. Balcells G. de la Fuente M. Tor J. Gómez-Arrue A.R. Seradj |
| author_sort | L. Sarri |
| collection | DOAJ |
| description | Differences in producing performance and organoleptic meat characteristics among pig genotypes and/or producing types are widely known. These parameters are also subjected to the animal’s development, feeding and management. Detailed knowledge of the effects of production phase (PP), pig producing type (PT), dietary protein availability and their interactions on nutrient digestibility, nitrogen balance and protein metabolism is essential information to improve precision feeding techniques. The experiment was a 2 (PP) × 2 (PT) × 2 (diet) factorial design conducted with 32 male pigs, 16 entire F2 pigs progeny of Pietrain sires and Duroc × Landrace dams, and 16 castrated purebred Durocs belonging to two production phases (growing: 29.5 ± 3.19 v. fattening: 88.6 ± 6.26 kg BW), and assigned to one of two dietary CP levels, either standard (SP: 17% in growing and 15% in fattening) or low (LP: 15% in growing and 13% in fattening). Viscera and muscle fractional protein synthesis rates (FSRs; %/day) were conducted through a single infusion of 15% L-[ring-2H5]-phenylalanine, with subsequent blood sampling from 12 to 40 min, and sample collection of liver, duodenum, biceps femoris and longissimus dorsi skeletal muscles after sacrifice. Fattening animals acquired a greater feed ingestion capacity, average daily gain (P < 0.01) and apparent ileal digestibility, whereas growing pigs showed higher FSRs in both viscera (duodenum and liver) and in longissimus dorsi. F2 pigs showed higher average daily gain, nitrogen retention rates and FSR in liver and longissimus dorsi (P < 0.01). Nevertheless, apparent ileal digestibility in all essential amino acids was lower in F2 compared with Duroc pigs (P < 0.05). Protein metabolism was barely influenced by dietary CP content, although animals fed LP registered the lowest apparent ileal digestibility for CP and also for most of the essential amino acids compared with SP-fed pigs. This information may reveal differences in amino acid requirements between both PTs, with Duroc pigs receiving excess of dietary amino acids. |
| first_indexed | 2024-12-16T09:35:13Z |
| format | Article |
| id | doaj.art-f0599583eb5f4110a768ed5c3fc029b6 |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-16T09:35:13Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-f0599583eb5f4110a768ed5c3fc029b62022-12-21T22:36:26ZengElsevierAnimal1751-73112021-06-01156100220Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restrictionL. Sarri0J. Balcells1G. de la Fuente2M. Tor3J. Gómez-Arrue4A.R. Seradj5Departament de Ciència Animal, Universitat de Lleida- Agrotecnio-CERCA Centre, Av. Alcalde Rovira Roure 191, 25198 Lleida, SpainDepartament de Ciència Animal, Universitat de Lleida- Agrotecnio-CERCA Centre, Av. Alcalde Rovira Roure 191, 25198 Lleida, SpainDepartament de Ciència Animal, Universitat de Lleida- Agrotecnio-CERCA Centre, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain; Corresponding author.Departament de Ciència Animal, Universitat de Lleida- Agrotecnio-CERCA Centre, Av. Alcalde Rovira Roure 191, 25198 Lleida, SpainInstituto Aragonés de Ciencias de la Salud (IACS), Av. San Juan Bosco 13, 50009 Zaragoza, SpainDepartament de Ciència Animal, Universitat de Lleida- Agrotecnio-CERCA Centre, Av. Alcalde Rovira Roure 191, 25198 Lleida, SpainDifferences in producing performance and organoleptic meat characteristics among pig genotypes and/or producing types are widely known. These parameters are also subjected to the animal’s development, feeding and management. Detailed knowledge of the effects of production phase (PP), pig producing type (PT), dietary protein availability and their interactions on nutrient digestibility, nitrogen balance and protein metabolism is essential information to improve precision feeding techniques. The experiment was a 2 (PP) × 2 (PT) × 2 (diet) factorial design conducted with 32 male pigs, 16 entire F2 pigs progeny of Pietrain sires and Duroc × Landrace dams, and 16 castrated purebred Durocs belonging to two production phases (growing: 29.5 ± 3.19 v. fattening: 88.6 ± 6.26 kg BW), and assigned to one of two dietary CP levels, either standard (SP: 17% in growing and 15% in fattening) or low (LP: 15% in growing and 13% in fattening). Viscera and muscle fractional protein synthesis rates (FSRs; %/day) were conducted through a single infusion of 15% L-[ring-2H5]-phenylalanine, with subsequent blood sampling from 12 to 40 min, and sample collection of liver, duodenum, biceps femoris and longissimus dorsi skeletal muscles after sacrifice. Fattening animals acquired a greater feed ingestion capacity, average daily gain (P < 0.01) and apparent ileal digestibility, whereas growing pigs showed higher FSRs in both viscera (duodenum and liver) and in longissimus dorsi. F2 pigs showed higher average daily gain, nitrogen retention rates and FSR in liver and longissimus dorsi (P < 0.01). Nevertheless, apparent ileal digestibility in all essential amino acids was lower in F2 compared with Duroc pigs (P < 0.05). Protein metabolism was barely influenced by dietary CP content, although animals fed LP registered the lowest apparent ileal digestibility for CP and also for most of the essential amino acids compared with SP-fed pigs. This information may reveal differences in amino acid requirements between both PTs, with Duroc pigs receiving excess of dietary amino acids.http://www.sciencedirect.com/science/article/pii/S1751731121000628Apparent digestibilityPig producing typeProduction phaseProtein restrictionProtein synthesis |
| spellingShingle | L. Sarri J. Balcells G. de la Fuente M. Tor J. Gómez-Arrue A.R. Seradj Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction Animal Apparent digestibility Pig producing type Production phase Protein restriction Protein synthesis |
| title | Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction |
| title_full | Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction |
| title_fullStr | Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction |
| title_full_unstemmed | Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction |
| title_short | Evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated Duroc pigs fed under moderate crude protein restriction |
| title_sort | evolution of viscera and muscle fractional protein synthesis rate in lean meat selected hybrids and castrated duroc pigs fed under moderate crude protein restriction |
| topic | Apparent digestibility Pig producing type Production phase Protein restriction Protein synthesis |
| url | http://www.sciencedirect.com/science/article/pii/S1751731121000628 |
| work_keys_str_mv | AT lsarri evolutionofvisceraandmusclefractionalproteinsynthesisrateinleanmeatselectedhybridsandcastrateddurocpigsfedundermoderatecrudeproteinrestriction AT jbalcells evolutionofvisceraandmusclefractionalproteinsynthesisrateinleanmeatselectedhybridsandcastrateddurocpigsfedundermoderatecrudeproteinrestriction AT gdelafuente evolutionofvisceraandmusclefractionalproteinsynthesisrateinleanmeatselectedhybridsandcastrateddurocpigsfedundermoderatecrudeproteinrestriction AT mtor evolutionofvisceraandmusclefractionalproteinsynthesisrateinleanmeatselectedhybridsandcastrateddurocpigsfedundermoderatecrudeproteinrestriction AT jgomezarrue evolutionofvisceraandmusclefractionalproteinsynthesisrateinleanmeatselectedhybridsandcastrateddurocpigsfedundermoderatecrudeproteinrestriction AT arseradj evolutionofvisceraandmusclefractionalproteinsynthesisrateinleanmeatselectedhybridsandcastrateddurocpigsfedundermoderatecrudeproteinrestriction |