Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens
Marine algae are sources of bioactive components with defensive properties of great value against microbial infections. This study investigated the bioactivity of extracts from brown algae <i>Fucus vesiculosus</i> against rotavirus, the worldwide leading cause of acute gastroenteritis in...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/9/478 |
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| author | Dimitra Graikini Arturo B. Soro Saravana P. Sivagnanam Brijesh K. Tiwari Lourdes Sánchez |
| author_facet | Dimitra Graikini Arturo B. Soro Saravana P. Sivagnanam Brijesh K. Tiwari Lourdes Sánchez |
| author_sort | Dimitra Graikini |
| collection | DOAJ |
| description | Marine algae are sources of bioactive components with defensive properties of great value against microbial infections. This study investigated the bioactivity of extracts from brown algae <i>Fucus vesiculosus</i> against rotavirus, the worldwide leading cause of acute gastroenteritis in infants and young children. Moreover, one of the extracts was tested against four foodborne bacteria: <i>Campylobacter jejuni</i>, <i>Escherichia coli</i>, <i>Salmonella</i> Typhimurium, and <i>Listeria monocytogenes</i>, and the non-pathogenic: <i>E. coli</i> K12. In vitro tests using MA104 cells revealed that both whole algae extracts and crude fucoidan precipitates neutralized rotavirus in a dose-responsive manner. The maximum neutralization activity was observed when the rotavirus was incubated with 100 μg mL<sup>−1</sup> of the hydrochloric acid-obtained crude fucoidan (91.8%), although crude fucoidan extracted using citric acid also demonstrated high values (89.5%) at the same concentration. Furthermore, molecular weight fractionation of extracts decreased their antirotaviral activity and high molecular weight fractions exhibited higher activity compared to those of lower molecular weight. A seaweed extract with high antirotaviral activity was also found to inhibit the growth of <i>C. jejuni</i>, <i>S</i>. Typhimurium, and <i>L. monocytogenes</i> at a concentration of 0.2 mg mL<sup>−1</sup>. Overall, this study expands the current knowledge regarding the antimicrobial mechanisms of action of extracts from <i>F. vesiculosus</i>. |
| first_indexed | 2024-03-10T22:31:34Z |
| format | Article |
| id | doaj.art-f05c8f0aaa6c43d28c5ad7536df49b3a |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T22:31:34Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f05c8f0aaa6c43d28c5ad7536df49b3a2023-11-19T11:42:09ZengMDPI AGMarine Drugs1660-33972023-08-0121947810.3390/md21090478Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne PathogensDimitra Graikini0Arturo B. Soro1Saravana P. Sivagnanam2Brijesh K. Tiwari3Lourdes Sánchez4Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainFoodborne Pathogens Unit, Department of Infectious Diseases in Humans, Sciensano, 1050 Brussels, BelgiumTeagasc Ashtown Food Research Centre, D15 DY05 Dublin, IrelandTeagasc Ashtown Food Research Centre, D15 DY05 Dublin, IrelandDepartamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainMarine algae are sources of bioactive components with defensive properties of great value against microbial infections. This study investigated the bioactivity of extracts from brown algae <i>Fucus vesiculosus</i> against rotavirus, the worldwide leading cause of acute gastroenteritis in infants and young children. Moreover, one of the extracts was tested against four foodborne bacteria: <i>Campylobacter jejuni</i>, <i>Escherichia coli</i>, <i>Salmonella</i> Typhimurium, and <i>Listeria monocytogenes</i>, and the non-pathogenic: <i>E. coli</i> K12. In vitro tests using MA104 cells revealed that both whole algae extracts and crude fucoidan precipitates neutralized rotavirus in a dose-responsive manner. The maximum neutralization activity was observed when the rotavirus was incubated with 100 μg mL<sup>−1</sup> of the hydrochloric acid-obtained crude fucoidan (91.8%), although crude fucoidan extracted using citric acid also demonstrated high values (89.5%) at the same concentration. Furthermore, molecular weight fractionation of extracts decreased their antirotaviral activity and high molecular weight fractions exhibited higher activity compared to those of lower molecular weight. A seaweed extract with high antirotaviral activity was also found to inhibit the growth of <i>C. jejuni</i>, <i>S</i>. Typhimurium, and <i>L. monocytogenes</i> at a concentration of 0.2 mg mL<sup>−1</sup>. Overall, this study expands the current knowledge regarding the antimicrobial mechanisms of action of extracts from <i>F. vesiculosus</i>.https://www.mdpi.com/1660-3397/21/9/478<i>Fucus vesiculosus</i>fucoidansgreen extractionrotavirusantirotaviral activityantibacterial assay |
| spellingShingle | Dimitra Graikini Arturo B. Soro Saravana P. Sivagnanam Brijesh K. Tiwari Lourdes Sánchez Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens Marine Drugs <i>Fucus vesiculosus</i> fucoidans green extraction rotavirus antirotaviral activity antibacterial assay |
| title | Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens |
| title_full | Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens |
| title_fullStr | Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens |
| title_full_unstemmed | Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens |
| title_short | Bioactivity of Fucoidan-Rich Extracts from <i>Fucus vesiculosus</i> against Rotavirus and Foodborne Pathogens |
| title_sort | bioactivity of fucoidan rich extracts from i fucus vesiculosus i against rotavirus and foodborne pathogens |
| topic | <i>Fucus vesiculosus</i> fucoidans green extraction rotavirus antirotaviral activity antibacterial assay |
| url | https://www.mdpi.com/1660-3397/21/9/478 |
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