The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i>
Pathogens have to cope with oxidative, iron- and carbon(glucose)-limitation stresses in the human body. To understand how combined iron–carbon limitation alters oxidative stress responses, <i>Aspergillus fumigatus</i> was cultured in glucose–peptone or peptone containing media supplement...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/10/3/221 |
| _version_ | 1827305879447273472 |
|---|---|
| author | Tamás Emri Károly Antal Kinga Varga Barnabás Csaba Gila István Pócsi |
| author_facet | Tamás Emri Károly Antal Kinga Varga Barnabás Csaba Gila István Pócsi |
| author_sort | Tamás Emri |
| collection | DOAJ |
| description | Pathogens have to cope with oxidative, iron- and carbon(glucose)-limitation stresses in the human body. To understand how combined iron–carbon limitation alters oxidative stress responses, <i>Aspergillus fumigatus</i> was cultured in glucose–peptone or peptone containing media supplemented or not with deferiprone as an iron chelator. Changes in the transcriptome in these cultures were recorded after H<sub>2</sub>O<sub>2</sub> treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. Out of the 16 stress responsive antioxidative enzyme genes, only the <i>cat2</i> catalase–peroxidase gene was upregulated in more than two culturing conditions. The transcriptional responses observed in iron metabolism also varied substantially in these cultures. Only extracellular siderophore production appeared important regardless of culturing conditions in oxidative stress protection, while the enhanced synthesis of Fe-S cluster proteins seemed to be crucial for oxidative stress treated iron-limited and fast growing (glucose rich) cultures. Although pathogens and host cells live together in the same place, their culturing conditions (e.g., iron availability or occurrence of oxidative stress) can be different. Therefore, inhibition of a universally important biochemical process, like Fe-S cluster assembly, may selectively inhibit the pathogen growth in vivo and represent a potential target for antifungal therapy. |
| first_indexed | 2024-04-24T18:07:33Z |
| format | Article |
| id | doaj.art-f060f6653e23402d98f2dea7eb853fab |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-04-24T18:07:33Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-f060f6653e23402d98f2dea7eb853fab2024-03-27T13:49:44ZengMDPI AGJournal of Fungi2309-608X2024-03-0110322110.3390/jof10030221The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i>Tamás Emri0Károly Antal1Kinga Varga2Barnabás Csaba Gila3István Pócsi4Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Zoology, Eszterházy Károly Catholic University, H-3300 Eger, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, HungaryPathogens have to cope with oxidative, iron- and carbon(glucose)-limitation stresses in the human body. To understand how combined iron–carbon limitation alters oxidative stress responses, <i>Aspergillus fumigatus</i> was cultured in glucose–peptone or peptone containing media supplemented or not with deferiprone as an iron chelator. Changes in the transcriptome in these cultures were recorded after H<sub>2</sub>O<sub>2</sub> treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. Out of the 16 stress responsive antioxidative enzyme genes, only the <i>cat2</i> catalase–peroxidase gene was upregulated in more than two culturing conditions. The transcriptional responses observed in iron metabolism also varied substantially in these cultures. Only extracellular siderophore production appeared important regardless of culturing conditions in oxidative stress protection, while the enhanced synthesis of Fe-S cluster proteins seemed to be crucial for oxidative stress treated iron-limited and fast growing (glucose rich) cultures. Although pathogens and host cells live together in the same place, their culturing conditions (e.g., iron availability or occurrence of oxidative stress) can be different. Therefore, inhibition of a universally important biochemical process, like Fe-S cluster assembly, may selectively inhibit the pathogen growth in vivo and represent a potential target for antifungal therapy.https://www.mdpi.com/2309-608X/10/3/221<i>Aspergillus fumigatus</i>carbon limitationdeferiproneiron homeostasisoxidative stressRNA sequencing |
| spellingShingle | Tamás Emri Károly Antal Kinga Varga Barnabás Csaba Gila István Pócsi The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i> Journal of Fungi <i>Aspergillus fumigatus</i> carbon limitation deferiprone iron homeostasis oxidative stress RNA sequencing |
| title | The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i> |
| title_full | The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i> |
| title_fullStr | The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i> |
| title_full_unstemmed | The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i> |
| title_short | The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in <i>Aspergillus fumigatus</i> |
| title_sort | oxidative stress response highly depends on glucose and iron availability in i aspergillus fumigatus i |
| topic | <i>Aspergillus fumigatus</i> carbon limitation deferiprone iron homeostasis oxidative stress RNA sequencing |
| url | https://www.mdpi.com/2309-608X/10/3/221 |
| work_keys_str_mv | AT tamasemri theoxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT karolyantal theoxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT kingavarga theoxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT barnabascsabagila theoxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT istvanpocsi theoxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT tamasemri oxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT karolyantal oxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT kingavarga oxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT barnabascsabagila oxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi AT istvanpocsi oxidativestressresponsehighlydependsonglucoseandironavailabilityiniaspergillusfumigatusi |