Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas
The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrif...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/9/4/326 |
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| author | Olga Senko Marina Gladchenko Olga Maslova Elena Efremenko |
| author_facet | Olga Senko Marina Gladchenko Olga Maslova Elena Efremenko |
| author_sort | Olga Senko |
| collection | DOAJ |
| description | The aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional„ introduction of bacterial <i>Clostridium acetobutylicum</i>, <i>Pseudomonas</i> sp., <i>Enterococcus faecalis</i> cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge. |
| first_indexed | 2024-04-11T23:43:55Z |
| format | Article |
| id | doaj.art-f06136d98c1540b99f01b3d1c4427843 |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-04-11T23:43:55Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Catalysts |
| spelling | doaj.art-f06136d98c1540b99f01b3d1c44278432022-12-22T03:56:43ZengMDPI AGCatalysts2073-43442019-04-019432610.3390/catal9040326catal9040326Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to BiogasOlga Senko0Marina Gladchenko1Olga Maslova2Elena Efremenko3Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaThe aim of this paper is to demonstrate the possibilities of anaerobic sludge cells immobilized into poly(vinyl alcohol) cryogel for the methanogenic conversion of various lignocellulosic waste and other media containing antibiotics (ampicillin, kanamycin, benzylpenicillin) or pesticides (chlorpyrifos or methiocarb and its derivatives). It was established that the immobilized cells of the anaerobic consortium can be stored frozen for at least three years while preserving a high level of metabolic activity. The cells after the long-term storage in an immobilized and frozen state were applied for the methanogenesis of a wide number of wastes, and an increase in both methane yield and methane portion in the produced biogas as compared to the conventionally used suspended anaerobic sludge cells, was ensured. It was shown that the “additional„ introduction of bacterial <i>Clostridium acetobutylicum</i>, <i>Pseudomonas</i> sp., <i>Enterococcus faecalis</i> cells (also immobilized using same support) improves characteristics of methanogenesis catalyzed by immobilized anaerobic sludge.https://www.mdpi.com/2073-4344/9/4/326immobilizationmethanogenesislignocellulosic wastepesticideantibiotics |
| spellingShingle | Olga Senko Marina Gladchenko Olga Maslova Elena Efremenko Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas Catalysts immobilization methanogenesis lignocellulosic waste pesticide antibiotics |
| title | Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas |
| title_full | Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas |
| title_fullStr | Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas |
| title_full_unstemmed | Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas |
| title_short | Long-Term Storage and Use of Artificially Immobilized Anaerobic Sludge as a Powerful Biocatalyst for Conversion of Various Wastes Including Those Containing Xenobiotics to Biogas |
| title_sort | long term storage and use of artificially immobilized anaerobic sludge as a powerful biocatalyst for conversion of various wastes including those containing xenobiotics to biogas |
| topic | immobilization methanogenesis lignocellulosic waste pesticide antibiotics |
| url | https://www.mdpi.com/2073-4344/9/4/326 |
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