Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine
Abstract Dendritic cell (DC) vaccine was among the first FDA-approved cancer immunotherapies, but has been limited by the modest cytotoxic T lymphocyte (CTL) response and therapeutic efficacy. Here we report a facile metabolic labeling approach that enables targeted modulation of adoptively transfer...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40886-7 |
| _version_ | 1827710006695297024 |
|---|---|
| author | Joonsu Han Rimsha Bhatta Yusheng Liu Yang Bo Alberto Elosegui-Artola Hua Wang |
| author_facet | Joonsu Han Rimsha Bhatta Yusheng Liu Yang Bo Alberto Elosegui-Artola Hua Wang |
| author_sort | Joonsu Han |
| collection | DOAJ |
| description | Abstract Dendritic cell (DC) vaccine was among the first FDA-approved cancer immunotherapies, but has been limited by the modest cytotoxic T lymphocyte (CTL) response and therapeutic efficacy. Here we report a facile metabolic labeling approach that enables targeted modulation of adoptively transferred DCs for developing enhanced DC vaccines. We show that metabolic glycan labeling can reduce the membrane mobility of DCs, which activates DCs and improves the antigen presentation and subsequent T cell priming property of DCs. Metabolic glycan labeling itself can enhance the antitumor efficacy of DC vaccines. In addition, the cell-surface chemical tags (e.g., azido groups) introduced via metabolic glycan labeling also enable in vivo conjugation of cytokines onto adoptively transferred DCs, which further enhances CTL response and antitumor efficacy. Our DC labeling and targeting technology provides a strategy to improve the therapeutic efficacy of DC vaccines, with minimal interference upon the clinical manufacturing process. |
| first_indexed | 2024-03-10T17:31:09Z |
| format | Article |
| id | doaj.art-f06180e285c4431180104e3c9d6fba11 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:31:09Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f06180e285c4431180104e3c9d6fba112023-11-20T10:02:28ZengNature PortfolioNature Communications2041-17232023-08-0114111410.1038/s41467-023-40886-7Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccineJoonsu Han0Rimsha Bhatta1Yusheng Liu2Yang Bo3Alberto Elosegui-Artola4Hua Wang5Department of Materials Science and Engineering, University of Illinois at Urbana-ChampaignDepartment of Materials Science and Engineering, University of Illinois at Urbana-ChampaignDepartment of Materials Science and Engineering, University of Illinois at Urbana-ChampaignDepartment of Materials Science and Engineering, University of Illinois at Urbana-ChampaignCell and Tissue Mechanobiology Laboratory, Francis Crick InstituteDepartment of Materials Science and Engineering, University of Illinois at Urbana-ChampaignAbstract Dendritic cell (DC) vaccine was among the first FDA-approved cancer immunotherapies, but has been limited by the modest cytotoxic T lymphocyte (CTL) response and therapeutic efficacy. Here we report a facile metabolic labeling approach that enables targeted modulation of adoptively transferred DCs for developing enhanced DC vaccines. We show that metabolic glycan labeling can reduce the membrane mobility of DCs, which activates DCs and improves the antigen presentation and subsequent T cell priming property of DCs. Metabolic glycan labeling itself can enhance the antitumor efficacy of DC vaccines. In addition, the cell-surface chemical tags (e.g., azido groups) introduced via metabolic glycan labeling also enable in vivo conjugation of cytokines onto adoptively transferred DCs, which further enhances CTL response and antitumor efficacy. Our DC labeling and targeting technology provides a strategy to improve the therapeutic efficacy of DC vaccines, with minimal interference upon the clinical manufacturing process.https://doi.org/10.1038/s41467-023-40886-7 |
| spellingShingle | Joonsu Han Rimsha Bhatta Yusheng Liu Yang Bo Alberto Elosegui-Artola Hua Wang Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine Nature Communications |
| title | Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine |
| title_full | Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine |
| title_fullStr | Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine |
| title_full_unstemmed | Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine |
| title_short | Metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine |
| title_sort | metabolic glycan labeling immobilizes dendritic cell membrane and enhances antitumor efficacy of dendritic cell vaccine |
| url | https://doi.org/10.1038/s41467-023-40886-7 |
| work_keys_str_mv | AT joonsuhan metabolicglycanlabelingimmobilizesdendriticcellmembraneandenhancesantitumorefficacyofdendriticcellvaccine AT rimshabhatta metabolicglycanlabelingimmobilizesdendriticcellmembraneandenhancesantitumorefficacyofdendriticcellvaccine AT yushengliu metabolicglycanlabelingimmobilizesdendriticcellmembraneandenhancesantitumorefficacyofdendriticcellvaccine AT yangbo metabolicglycanlabelingimmobilizesdendriticcellmembraneandenhancesantitumorefficacyofdendriticcellvaccine AT albertoeloseguiartola metabolicglycanlabelingimmobilizesdendriticcellmembraneandenhancesantitumorefficacyofdendriticcellvaccine AT huawang metabolicglycanlabelingimmobilizesdendriticcellmembraneandenhancesantitumorefficacyofdendriticcellvaccine |