Ionizing radiation and bone quality: time-dependent effects
Abstract Background The aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation. Methods Wistar rats were submitted to IR (30 Gy) on the left leg and were euthanized after 30 and 60 days. The legs were divided into four groups according to t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-01-01
|
| Series: | Radiation Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13014-019-1219-y |
| _version_ | 1818034695678459904 |
|---|---|
| author | Pedro Henrique Justino Oliveira Limirio Priscilla Barbosa Ferreira Soares Eduardo Tadashi Pinto Emi Camila de Carvalho Almança Lopes Flaviana Soares Rocha Jonas Dantas Batista Gustavo Davi Rabelo Paula Dechichi |
| author_facet | Pedro Henrique Justino Oliveira Limirio Priscilla Barbosa Ferreira Soares Eduardo Tadashi Pinto Emi Camila de Carvalho Almança Lopes Flaviana Soares Rocha Jonas Dantas Batista Gustavo Davi Rabelo Paula Dechichi |
| author_sort | Pedro Henrique Justino Oliveira Limirio |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation. Methods Wistar rats were submitted to IR (30 Gy) on the left leg and were euthanized after 30 and 60 days. The legs were divided into four groups according to the treatment and euthanization time: C30 and C60 (right leg–without IR), IR30 and IR60 (left leg-with IR). Results CT analysis showed more radiodensity in C60 compared with other groups, and IR60 showed more radiodensity than IR30. In histomorphometric analysis, C30 showed lower bone matrix values compared with IR30 and C60. Lacunarity analyses showed more homogeneous bone channel distribution in C30 than IR30. ATR-FTIR showed decrease in ratio of mature and immature crosslinks in IR30 compared with C30. Crystallinity Index was decrease in IR60 compared with C60. The Amide III + Collagen/HA ratio was increased in C60 compared with C30; however this ratio decreased in IR60 compared with IR30. Biomechanical analysis showed lower values in IR groups in both time. Conclusions IR damaged bone quality and decreased stiffness. Moreover, the results suggested that the deleterious effects of IR increased in the late time points. |
| first_indexed | 2024-12-10T06:43:15Z |
| format | Article |
| id | doaj.art-f06317d10f4e48aba079a6603f78e92e |
| institution | Directory Open Access Journal |
| issn | 1748-717X |
| language | English |
| last_indexed | 2024-12-10T06:43:15Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Radiation Oncology |
| spelling | doaj.art-f06317d10f4e48aba079a6603f78e92e2022-12-22T01:58:44ZengBMCRadiation Oncology1748-717X2019-01-011411810.1186/s13014-019-1219-yIonizing radiation and bone quality: time-dependent effectsPedro Henrique Justino Oliveira Limirio0Priscilla Barbosa Ferreira Soares1Eduardo Tadashi Pinto Emi2Camila de Carvalho Almança Lopes3Flaviana Soares Rocha4Jonas Dantas Batista5Gustavo Davi Rabelo6Paula Dechichi7Integrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaIntegrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaIntegrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaIntegrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaIntegrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaIntegrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaFaculty of Dentistry, Federal University of Juiz de ForaIntegrated Dental Clinic Program, Faculty of Dentistry, Federal University of UberlândiaAbstract Background The aim of this study was to evaluate the ionizing radiation (IR) effects on rat bone 30 and 60 days after irradiation. Methods Wistar rats were submitted to IR (30 Gy) on the left leg and were euthanized after 30 and 60 days. The legs were divided into four groups according to the treatment and euthanization time: C30 and C60 (right leg–without IR), IR30 and IR60 (left leg-with IR). Results CT analysis showed more radiodensity in C60 compared with other groups, and IR60 showed more radiodensity than IR30. In histomorphometric analysis, C30 showed lower bone matrix values compared with IR30 and C60. Lacunarity analyses showed more homogeneous bone channel distribution in C30 than IR30. ATR-FTIR showed decrease in ratio of mature and immature crosslinks in IR30 compared with C30. Crystallinity Index was decrease in IR60 compared with C60. The Amide III + Collagen/HA ratio was increased in C60 compared with C30; however this ratio decreased in IR60 compared with IR30. Biomechanical analysis showed lower values in IR groups in both time. Conclusions IR damaged bone quality and decreased stiffness. Moreover, the results suggested that the deleterious effects of IR increased in the late time points.http://link.springer.com/article/10.1186/s13014-019-1219-yCortical boneRadiation, ionizingBiomechanicsBone matrix |
| spellingShingle | Pedro Henrique Justino Oliveira Limirio Priscilla Barbosa Ferreira Soares Eduardo Tadashi Pinto Emi Camila de Carvalho Almança Lopes Flaviana Soares Rocha Jonas Dantas Batista Gustavo Davi Rabelo Paula Dechichi Ionizing radiation and bone quality: time-dependent effects Radiation Oncology Cortical bone Radiation, ionizing Biomechanics Bone matrix |
| title | Ionizing radiation and bone quality: time-dependent effects |
| title_full | Ionizing radiation and bone quality: time-dependent effects |
| title_fullStr | Ionizing radiation and bone quality: time-dependent effects |
| title_full_unstemmed | Ionizing radiation and bone quality: time-dependent effects |
| title_short | Ionizing radiation and bone quality: time-dependent effects |
| title_sort | ionizing radiation and bone quality time dependent effects |
| topic | Cortical bone Radiation, ionizing Biomechanics Bone matrix |
| url | http://link.springer.com/article/10.1186/s13014-019-1219-y |
| work_keys_str_mv | AT pedrohenriquejustinooliveiralimirio ionizingradiationandbonequalitytimedependenteffects AT priscillabarbosaferreirasoares ionizingradiationandbonequalitytimedependenteffects AT eduardotadashipintoemi ionizingradiationandbonequalitytimedependenteffects AT camiladecarvalhoalmancalopes ionizingradiationandbonequalitytimedependenteffects AT flavianasoaresrocha ionizingradiationandbonequalitytimedependenteffects AT jonasdantasbatista ionizingradiationandbonequalitytimedependenteffects AT gustavodavirabelo ionizingradiationandbonequalitytimedependenteffects AT pauladechichi ionizingradiationandbonequalitytimedependenteffects |