Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography
Summary: A substance that sexualizes planarians, an ancestral group of parasitic flatworms, is widely present in planarians and parasitic flatworms. Here, we present a protocol for extracting and purifying the active fraction with sex-inducing activity. We describe steps for homogenization of flatwo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723005920 |
| _version_ | 1797647587138863104 |
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| author | Kiyono Sekii Taro Watanabe Riku Ito Akitoshi Yoshikawa Madoka Ichikawa-Seki Kimitoshi Sakamoto Kazuya Kobayashi |
| author_facet | Kiyono Sekii Taro Watanabe Riku Ito Akitoshi Yoshikawa Madoka Ichikawa-Seki Kimitoshi Sakamoto Kazuya Kobayashi |
| author_sort | Kiyono Sekii |
| collection | DOAJ |
| description | Summary: A substance that sexualizes planarians, an ancestral group of parasitic flatworms, is widely present in planarians and parasitic flatworms. Here, we present a protocol for extracting and purifying the active fraction with sex-inducing activity. We describe steps for homogenization of flatworms, sample concentration, open-column chromatography, and reverse-phase high-performance liquid chromatography. We then detail a feeding bioassay to confirm sex-inducing activity. The obtained active fraction may positively affect parasitic flatworm sexual maturation and can be tested by adding it into the culture media.For complete details on the use and execution of this protocol, please refer to Sekii et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-11T15:19:39Z |
| format | Article |
| id | doaj.art-f0636708335f49d29c107fbdc930e149 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-11T15:19:39Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f0636708335f49d29c107fbdc930e1492023-10-29T04:20:16ZengElsevierSTAR Protocols2666-16672023-12-0144102625Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatographyKiyono Sekii0Taro Watanabe1Riku Ito2Akitoshi Yoshikawa3Madoka Ichikawa-Seki4Kimitoshi Sakamoto5Kazuya Kobayashi6Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Faculty of Business and Commerce, Keio University, Hiyoshi 4-1-1, Yokohama, Kanagawa 223-8521, JapanFaculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, JapanFaculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, JapanFaculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, JapanLaboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, JapanFaculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Corresponding authorFaculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Corresponding authorSummary: A substance that sexualizes planarians, an ancestral group of parasitic flatworms, is widely present in planarians and parasitic flatworms. Here, we present a protocol for extracting and purifying the active fraction with sex-inducing activity. We describe steps for homogenization of flatworms, sample concentration, open-column chromatography, and reverse-phase high-performance liquid chromatography. We then detail a feeding bioassay to confirm sex-inducing activity. The obtained active fraction may positively affect parasitic flatworm sexual maturation and can be tested by adding it into the culture media.For complete details on the use and execution of this protocol, please refer to Sekii et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723005920Developmental BiologyBiotechnology and BioengineeringChemistry |
| spellingShingle | Kiyono Sekii Taro Watanabe Riku Ito Akitoshi Yoshikawa Madoka Ichikawa-Seki Kimitoshi Sakamoto Kazuya Kobayashi Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography STAR Protocols Developmental Biology Biotechnology and Bioengineering Chemistry |
| title | Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography |
| title_full | Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography |
| title_fullStr | Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography |
| title_full_unstemmed | Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography |
| title_short | Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography |
| title_sort | fractionation of a sex inducing substance from flatworms using open column chromatography and reverse phase high performance liquid chromatography |
| topic | Developmental Biology Biotechnology and Bioengineering Chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723005920 |
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