Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines
In the present study, silver nanoparticles (Ag-NPs) were synthesized using <i>Azadirachta indica</i> extract and evaluated for their in vitro antioxidant activity and cytotoxicity efficacy against MCF-7 and HeLa cells. The silver nanoparticles (Ag-NPs) were formed within 40 min and after...
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MDPI AG
2022-08-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/9/1416 |
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| author | S. Anitha Kumari Anita K. Patlolla P. Madhusudhanachary |
| author_facet | S. Anitha Kumari Anita K. Patlolla P. Madhusudhanachary |
| author_sort | S. Anitha Kumari |
| collection | DOAJ |
| description | In the present study, silver nanoparticles (Ag-NPs) were synthesized using <i>Azadirachta indica</i> extract and evaluated for their in vitro antioxidant activity and cytotoxicity efficacy against MCF-7 and HeLa cells. The silver nanoparticles (Ag-NPs) were formed within 40 min and after preliminary confirmation by UV-visible spectroscopy (peak observed at 375 nm), they were characterized using a transmission electron microscope (TEM) and dynamic light scattering (DLS). The TEM images showed the spherical shape of the biosynthesized Ag-NPs with particle sizes in the range of 10 to 60 nm, and compositional analysis was carried out. The cytotoxicity and antioxidant activity of various concentrations of biosynthesized silver nanoparticles, <i>Azadirachta indica</i> extract, and a standard ranging from 0.2 to 1.0 mg/mL were evaluated. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) activity of the biosynthesized Ag-NPs and aqueous leaf extract increased in a dose-dependent manner, with average IC<sub>50</sub> values of the biosynthesized Ag-NPs, aqueous leaf extract, and ascorbic acid (standard) of 0.70 ± 0.07, 1.63 ± 0.09, and 0.25 ± 0.09 mg/mL, respectively. Furthermore, higher cytotoxicity was exhibited in both the MCF-7 and HeLa cell lines in a dose-dependent manner. The average IC<sub>50</sub> values of the biosynthesized Ag-NPs, aqueous leaf extract, and cisplatin (standard) were 0.90 ± 0.07, 1.85 ± 0.01, and 0.56 ± 0.08 mg/mL, respectively, with MCF-7 cell lines and 0.85 ± 0.01, 1.76 ± 0.08, 0.45 ± 0.10 mg/mL, respectively, with HeLa cell lines. Hence, this study resulted in an efficient green reductant for producing silver nanoparticles that possess cytotoxicity and antioxidant activity against MCF-7 and HeLa cells. |
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| language | English |
| last_indexed | 2024-03-09T23:09:19Z |
| publishDate | 2022-08-01 |
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| series | Micromachines |
| spelling | doaj.art-f06371f1a79543b5acee154e55fc9d942023-11-23T17:49:10ZengMDPI AGMicromachines2072-666X2022-08-01139141610.3390/mi13091416Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell LinesS. Anitha Kumari0Anita K. Patlolla1P. Madhusudhanachary2Department of Zoology, Osmania University for Women, Hyderabad 500095, IndiaRCMI Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, Jackson, MS 39217, USAUltrastructure Unit, National Institute of Nutrition, Hyderabad-500095, IndiaIn the present study, silver nanoparticles (Ag-NPs) were synthesized using <i>Azadirachta indica</i> extract and evaluated for their in vitro antioxidant activity and cytotoxicity efficacy against MCF-7 and HeLa cells. The silver nanoparticles (Ag-NPs) were formed within 40 min and after preliminary confirmation by UV-visible spectroscopy (peak observed at 375 nm), they were characterized using a transmission electron microscope (TEM) and dynamic light scattering (DLS). The TEM images showed the spherical shape of the biosynthesized Ag-NPs with particle sizes in the range of 10 to 60 nm, and compositional analysis was carried out. The cytotoxicity and antioxidant activity of various concentrations of biosynthesized silver nanoparticles, <i>Azadirachta indica</i> extract, and a standard ranging from 0.2 to 1.0 mg/mL were evaluated. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) activity of the biosynthesized Ag-NPs and aqueous leaf extract increased in a dose-dependent manner, with average IC<sub>50</sub> values of the biosynthesized Ag-NPs, aqueous leaf extract, and ascorbic acid (standard) of 0.70 ± 0.07, 1.63 ± 0.09, and 0.25 ± 0.09 mg/mL, respectively. Furthermore, higher cytotoxicity was exhibited in both the MCF-7 and HeLa cell lines in a dose-dependent manner. The average IC<sub>50</sub> values of the biosynthesized Ag-NPs, aqueous leaf extract, and cisplatin (standard) were 0.90 ± 0.07, 1.85 ± 0.01, and 0.56 ± 0.08 mg/mL, respectively, with MCF-7 cell lines and 0.85 ± 0.01, 1.76 ± 0.08, 0.45 ± 0.10 mg/mL, respectively, with HeLa cell lines. Hence, this study resulted in an efficient green reductant for producing silver nanoparticles that possess cytotoxicity and antioxidant activity against MCF-7 and HeLa cells.https://www.mdpi.com/2072-666X/13/9/1416<i>Azadirachta indica</i>green synthesissilver nanoparticlesDPPHMTT |
| spellingShingle | S. Anitha Kumari Anita K. Patlolla P. Madhusudhanachary Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines Micromachines <i>Azadirachta indica</i> green synthesis silver nanoparticles DPPH MTT |
| title | Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines |
| title_full | Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines |
| title_fullStr | Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines |
| title_full_unstemmed | Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines |
| title_short | Biosynthesis of Silver Nanoparticles Using <i>Azadirachta indica</i> and Their Antioxidant and Anticancer Effects in Cell Lines |
| title_sort | biosynthesis of silver nanoparticles using i azadirachta indica i and their antioxidant and anticancer effects in cell lines |
| topic | <i>Azadirachta indica</i> green synthesis silver nanoparticles DPPH MTT |
| url | https://www.mdpi.com/2072-666X/13/9/1416 |
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