BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae)
<p>Abstract</p> <p>Background</p> <p>In animals, signaling of Bone Morphogenetic Proteins (BMPs) is essential for dorsoventral (DV) patterning of the embryo, but how BMP signaling evolved with changes in embryonic DV differentiation is largely unclear. Based on the exte...
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BMC
2011-05-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/278 |
| _version_ | 1818787549667131392 |
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| author | Domanus Marc H Meyer Folker Antonopoulos Dionysios A Lemke Steffen Schmidt-Ott Urs |
| author_facet | Domanus Marc H Meyer Folker Antonopoulos Dionysios A Lemke Steffen Schmidt-Ott Urs |
| author_sort | Domanus Marc H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In animals, signaling of Bone Morphogenetic Proteins (BMPs) is essential for dorsoventral (DV) patterning of the embryo, but how BMP signaling evolved with changes in embryonic DV differentiation is largely unclear. Based on the extensive knowledge of BMP signaling in <it>Drosophila melanogaster</it>, the morphological diversity of extraembryonic tissues in different fly species provides a comparative system to address this question. The closest relatives of <it>D. melanogaster </it>with clearly distinct DV differentiation are hover flies (Diptera: Syrphidae). The syrphid <it>Episyrphus balteatus </it>is a commercial bio-agent against aphids and has been established as a model organism for developmental studies and chemical ecology. The dorsal blastoderm of <it>E. balteatus </it>gives rise to two extraembryonic tissues (serosa and amnion), whereas in <it>D. melanogaster</it>, the dorsal blastoderm differentiates into a single extraembryonic epithelium (amnioserosa). Recent studies indicate that several BMP signaling components of <it>D. melanogaster</it>, including the BMP ligand Screw (Scw) and other extracellular regulators, evolved in the dipteran lineage through gene duplication and functional divergence. These findings raise the question of whether the complement of BMP signaling components changed with the origin of the amnioserosa.</p> <p>Results</p> <p>To search for BMP signaling components in <it>E. balteatus</it>, we generated and analyzed transcriptomes of freshly laid eggs (0-30 minutes) and late blastoderm to early germband extension stages (3-6 hours) using Roche/454 sequencing. We identified putative <it>E. balteatus </it>orthologues of 43% of all annotated <it>D. melanogaster </it>genes, including the genes of all BMP ligands and other BMP signaling components.</p> <p>Conclusion</p> <p>The diversification of several BMP signaling components in the dipteran linage of <it>D. melanogaster </it>preceded the origin of the amnioserosa.</p> <p>[Transcriptome sequence data from this study have been deposited at the NCBI Sequence Read Archive (SRP005289); individually assembled sequences have been deposited at GenBank (<ext-link ext-link-id="JN006969" ext-link-type="gen">JN006969</ext-link>-<ext-link ext-link-id="JN006986" ext-link-type="gen">JN006986</ext-link>).]</p> |
| first_indexed | 2024-12-18T14:09:33Z |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
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| last_indexed | 2024-12-18T14:09:33Z |
| publishDate | 2011-05-01 |
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| spelling | doaj.art-f06525b3ab3447ecac7c724af8d64f862022-12-21T21:05:10ZengBMCBMC Genomics1471-21642011-05-0112127810.1186/1471-2164-12-278BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae)Domanus Marc HMeyer FolkerAntonopoulos Dionysios ALemke SteffenSchmidt-Ott Urs<p>Abstract</p> <p>Background</p> <p>In animals, signaling of Bone Morphogenetic Proteins (BMPs) is essential for dorsoventral (DV) patterning of the embryo, but how BMP signaling evolved with changes in embryonic DV differentiation is largely unclear. Based on the extensive knowledge of BMP signaling in <it>Drosophila melanogaster</it>, the morphological diversity of extraembryonic tissues in different fly species provides a comparative system to address this question. The closest relatives of <it>D. melanogaster </it>with clearly distinct DV differentiation are hover flies (Diptera: Syrphidae). The syrphid <it>Episyrphus balteatus </it>is a commercial bio-agent against aphids and has been established as a model organism for developmental studies and chemical ecology. The dorsal blastoderm of <it>E. balteatus </it>gives rise to two extraembryonic tissues (serosa and amnion), whereas in <it>D. melanogaster</it>, the dorsal blastoderm differentiates into a single extraembryonic epithelium (amnioserosa). Recent studies indicate that several BMP signaling components of <it>D. melanogaster</it>, including the BMP ligand Screw (Scw) and other extracellular regulators, evolved in the dipteran lineage through gene duplication and functional divergence. These findings raise the question of whether the complement of BMP signaling components changed with the origin of the amnioserosa.</p> <p>Results</p> <p>To search for BMP signaling components in <it>E. balteatus</it>, we generated and analyzed transcriptomes of freshly laid eggs (0-30 minutes) and late blastoderm to early germband extension stages (3-6 hours) using Roche/454 sequencing. We identified putative <it>E. balteatus </it>orthologues of 43% of all annotated <it>D. melanogaster </it>genes, including the genes of all BMP ligands and other BMP signaling components.</p> <p>Conclusion</p> <p>The diversification of several BMP signaling components in the dipteran linage of <it>D. melanogaster </it>preceded the origin of the amnioserosa.</p> <p>[Transcriptome sequence data from this study have been deposited at the NCBI Sequence Read Archive (SRP005289); individually assembled sequences have been deposited at GenBank (<ext-link ext-link-id="JN006969" ext-link-type="gen">JN006969</ext-link>-<ext-link ext-link-id="JN006986" ext-link-type="gen">JN006986</ext-link>).]</p>http://www.biomedcentral.com/1471-2164/12/278 |
| spellingShingle | Domanus Marc H Meyer Folker Antonopoulos Dionysios A Lemke Steffen Schmidt-Ott Urs BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae) BMC Genomics |
| title | BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae) |
| title_full | BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae) |
| title_fullStr | BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae) |
| title_full_unstemmed | BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae) |
| title_short | BMP signaling components in embryonic transcriptomes of the hover fly <it>Episyrphus balteatus </it>(Syrphidae) |
| title_sort | bmp signaling components in embryonic transcriptomes of the hover fly it episyrphus balteatus it syrphidae |
| url | http://www.biomedcentral.com/1471-2164/12/278 |
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