Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea
Dispersants have been used in several oil spill accidents, but little information is available on their effectiveness in Baltic Sea conditions with low salinity and cold seawater. This study investigated the effects of dispersant use on petroleum hydrocarbon biodegradation rates and bacterial commun...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/882 |
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| author | Ossi Tonteri Anna Reunamo Aura Nousiainen Laura Koskinen Jari Nuutinen Jaak Truu Kirsten S. Jørgensen |
| author_facet | Ossi Tonteri Anna Reunamo Aura Nousiainen Laura Koskinen Jari Nuutinen Jaak Truu Kirsten S. Jørgensen |
| author_sort | Ossi Tonteri |
| collection | DOAJ |
| description | Dispersants have been used in several oil spill accidents, but little information is available on their effectiveness in Baltic Sea conditions with low salinity and cold seawater. This study investigated the effects of dispersant use on petroleum hydrocarbon biodegradation rates and bacterial community structures. Microcosm experiments were conducted at 5 °C for 12 days with North Sea crude oil and dispersant Finasol 51 with open sea Gulf of Bothnia and coastal Gulf of Finland and Norwegian Sea seawater. Petroleum hydrocarbon concentrations were analysed with GC-FID. Bacterial community structures were studied using 16S rDNA gene amplicon sequencing, and the abundance of genes involved in hydrocarbon degradation with quantitative PCR. The highest oil degradation gene abundances and oil removal were observed in microcosms with coastal seawater from the Gulf of Bothnia and Gulf of Finland, respectively, and the lowest in the seawater from the Norwegian Sea. Dispersant usage caused apparent effects on bacterial communities in all treatments; however, the dispersant’s effect on the biodegradation rate was unclear due to uncertainties with chemical analysis and variation in oil concentrations used in the experiments. |
| first_indexed | 2024-03-11T04:44:10Z |
| format | Article |
| id | doaj.art-f06559b272984b0a8d614b16f4b39480 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:44:10Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f06559b272984b0a8d614b16f4b394802023-11-17T20:31:54ZengMDPI AGMicroorganisms2076-26072023-03-0111488210.3390/microorganisms11040882Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian SeaOssi Tonteri0Anna Reunamo1Aura Nousiainen2Laura Koskinen3Jari Nuutinen4Jaak Truu5Kirsten S. Jørgensen6Marine Research Centre, Finnish Environmental Institute, Latokartanonkaari 11, FI-00790 Helsinki, FinlandMarine Research Centre, Finnish Environmental Institute, Latokartanonkaari 11, FI-00790 Helsinki, FinlandMarine Research Centre, Finnish Environmental Institute, Latokartanonkaari 11, FI-00790 Helsinki, FinlandLaboratory Centre, Finnish Environmental Institute, Latokartanonkaari 11, FI-00790 Helsinki, FinlandLaboratory Centre, Finnish Environmental Institute, Latokartanonkaari 11, FI-00790 Helsinki, FinlandInstitute of Molecular and Cell Biology, Faculty of Science and Technology, University of Tartu, Riia 23, 51010 Tartu, EstoniaMarine Research Centre, Finnish Environmental Institute, Latokartanonkaari 11, FI-00790 Helsinki, FinlandDispersants have been used in several oil spill accidents, but little information is available on their effectiveness in Baltic Sea conditions with low salinity and cold seawater. This study investigated the effects of dispersant use on petroleum hydrocarbon biodegradation rates and bacterial community structures. Microcosm experiments were conducted at 5 °C for 12 days with North Sea crude oil and dispersant Finasol 51 with open sea Gulf of Bothnia and coastal Gulf of Finland and Norwegian Sea seawater. Petroleum hydrocarbon concentrations were analysed with GC-FID. Bacterial community structures were studied using 16S rDNA gene amplicon sequencing, and the abundance of genes involved in hydrocarbon degradation with quantitative PCR. The highest oil degradation gene abundances and oil removal were observed in microcosms with coastal seawater from the Gulf of Bothnia and Gulf of Finland, respectively, and the lowest in the seawater from the Norwegian Sea. Dispersant usage caused apparent effects on bacterial communities in all treatments; however, the dispersant’s effect on the biodegradation rate was unclear due to uncertainties with chemical analysis and variation in oil concentrations used in the experiments.https://www.mdpi.com/2076-2607/11/4/882oil biodegradationdispersantBaltic SeaNorwegian Seaoil spillbacterial community |
| spellingShingle | Ossi Tonteri Anna Reunamo Aura Nousiainen Laura Koskinen Jari Nuutinen Jaak Truu Kirsten S. Jørgensen Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea Microorganisms oil biodegradation dispersant Baltic Sea Norwegian Sea oil spill bacterial community |
| title | Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea |
| title_full | Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea |
| title_fullStr | Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea |
| title_full_unstemmed | Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea |
| title_short | Effects of Dispersant on the Petroleum Hydrocarbon Biodegradation and Microbial Communities in Seawater from the Baltic Sea and Norwegian Sea |
| title_sort | effects of dispersant on the petroleum hydrocarbon biodegradation and microbial communities in seawater from the baltic sea and norwegian sea |
| topic | oil biodegradation dispersant Baltic Sea Norwegian Sea oil spill bacterial community |
| url | https://www.mdpi.com/2076-2607/11/4/882 |
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