Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions
<i>Rubia cordifolia</i> L. (Rubiaceae) is an important plant in Indian and Chinese medical systems. Extracts prepared from the root, stem and leaf have been used traditionally for the management of various diseases. Some of the known effects are anti-inflammation, neuroprotection, anti-p...
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MDPI AG
2022-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/1006 |
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| author | Ravikiran B. Humbare Joyita Sarkar Anjali A. Kulkarni Mugdha G. Juwale Sushil H. Deshmukh Dinesh Amalnerkar Manohar Chaskar Maria C. Albertini Marco B. L. Rocchi Swapnil C. Kamble Seeram Ramakrishna |
| author_facet | Ravikiran B. Humbare Joyita Sarkar Anjali A. Kulkarni Mugdha G. Juwale Sushil H. Deshmukh Dinesh Amalnerkar Manohar Chaskar Maria C. Albertini Marco B. L. Rocchi Swapnil C. Kamble Seeram Ramakrishna |
| author_sort | Ravikiran B. Humbare |
| collection | DOAJ |
| description | <i>Rubia cordifolia</i> L. (Rubiaceae) is an important plant in Indian and Chinese medical systems. Extracts prepared from the root, stem and leaf have been used traditionally for the management of various diseases. Some of the known effects are anti-inflammation, neuroprotection, anti-proliferation, immunomodulation and anti-tumor. A comparative account of the extracts derived from different organs that lead to the identification of the most suitable solvent is lacking. We explored the presence of phytochemicals, antioxidant activity and anti-proliferative properties of a variety of solvent-based extracts of root, and methanol extracts of stem and leaf of <i>R. cordifolia</i> L. The antioxidant potential was determined by DPPH, hydrogen peroxide, nitric oxide and total antioxidant assays. The anti-proliferative nature was evaluated by MTT assay on HeLa, ME-180 and HepG2 cells. The composition of the extracts was determined by UPLC-UV-MS. We found that the root extracts had the presence of higher amounts of antioxidants over the stem and leaf extracts. The root extracts prepared in methanol exhibited the highest cytotoxicity in HepG2 cells. The main compounds identified through UPLC-UV-MS of the methanol extract give credibility to the previous results. Our comprehensive study corroborates the preference given to the root over the stem and leaf for extract preparation. In conclusion, we identified the methanol extract of the root to be the most suited to have bioactivity with anti-cancer potential. |
| first_indexed | 2024-03-10T03:25:53Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:25:53Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f06770fccf634f5c880b99bbdccf7b712023-11-23T09:52:58ZengMDPI AGAntioxidants2076-39212022-05-01115100610.3390/antiox11051006Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction ConditionsRavikiran B. Humbare0Joyita Sarkar1Anjali A. Kulkarni2Mugdha G. Juwale3Sushil H. Deshmukh4Dinesh Amalnerkar5Manohar Chaskar6Maria C. Albertini7Marco B. L. Rocchi8Swapnil C. Kamble9Seeram Ramakrishna10Department of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, IndiaInstitute of Chemical Technology Mumbai, Marathwada Campus, Jalna, BT-6/7, Biotechnology Park, Additional MIDC Area, Aurangabad Road, Jalna 431203, IndiaDepartment of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, IndiaDepartment of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, IndiaMaharashtra Arogya Kendra’s Sane Guruji Arogya Kendra, Pune 411028, IndiaDepartment of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, IndiaDepartment of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, IndiaDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, IndiaCenter for Nanofibers and Nanotechnology, Department of Mechanical Engineering, National University of Singapore, Singapore 119260, Singapore<i>Rubia cordifolia</i> L. (Rubiaceae) is an important plant in Indian and Chinese medical systems. Extracts prepared from the root, stem and leaf have been used traditionally for the management of various diseases. Some of the known effects are anti-inflammation, neuroprotection, anti-proliferation, immunomodulation and anti-tumor. A comparative account of the extracts derived from different organs that lead to the identification of the most suitable solvent is lacking. We explored the presence of phytochemicals, antioxidant activity and anti-proliferative properties of a variety of solvent-based extracts of root, and methanol extracts of stem and leaf of <i>R. cordifolia</i> L. The antioxidant potential was determined by DPPH, hydrogen peroxide, nitric oxide and total antioxidant assays. The anti-proliferative nature was evaluated by MTT assay on HeLa, ME-180 and HepG2 cells. The composition of the extracts was determined by UPLC-UV-MS. We found that the root extracts had the presence of higher amounts of antioxidants over the stem and leaf extracts. The root extracts prepared in methanol exhibited the highest cytotoxicity in HepG2 cells. The main compounds identified through UPLC-UV-MS of the methanol extract give credibility to the previous results. Our comprehensive study corroborates the preference given to the root over the stem and leaf for extract preparation. In conclusion, we identified the methanol extract of the root to be the most suited to have bioactivity with anti-cancer potential.https://www.mdpi.com/2076-3921/11/5/1006secondary metabolitesanti-canceralternative medicinepolyvinylpolypyrrolidone (PVPP)phenol quenchingcell lines |
| spellingShingle | Ravikiran B. Humbare Joyita Sarkar Anjali A. Kulkarni Mugdha G. Juwale Sushil H. Deshmukh Dinesh Amalnerkar Manohar Chaskar Maria C. Albertini Marco B. L. Rocchi Swapnil C. Kamble Seeram Ramakrishna Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions Antioxidants secondary metabolites anti-cancer alternative medicine polyvinylpolypyrrolidone (PVPP) phenol quenching cell lines |
| title | Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions |
| title_full | Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions |
| title_fullStr | Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions |
| title_full_unstemmed | Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions |
| title_short | Phytochemical Characterization, Antioxidant and Anti-Proliferative Properties of <i>Rubia cordifolia</i> L. Extracts Prepared with Improved Extraction Conditions |
| title_sort | phytochemical characterization antioxidant and anti proliferative properties of i rubia cordifolia i l extracts prepared with improved extraction conditions |
| topic | secondary metabolites anti-cancer alternative medicine polyvinylpolypyrrolidone (PVPP) phenol quenching cell lines |
| url | https://www.mdpi.com/2076-3921/11/5/1006 |
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