Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
Plants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas...
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Format: | Article |
Language: | English |
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Bogor Agricultural University
2011-06-01
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Series: | Hayati Journal of Biosciences |
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Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/view/3432/2323 |
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author | ANTONIUS SUWANTO YULIN LESTARI ARIS TRI WAHYUDI ARI SUSILOWATI SURYO WIYONO |
author_facet | ANTONIUS SUWANTO YULIN LESTARI ARIS TRI WAHYUDI ARI SUSILOWATI SURYO WIYONO |
author_sort | ANTONIUS SUWANTO |
collection | DOAJ |
description | Plants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas sp. as biocontrol agent should be deeply explored. The aim of this study was to screen the indigenous soybeans’ rhizobacteria Pseudomonas sp. that possessing biocontrol characters against soilborne mainly i.e. Sclerotium rolfsii, Fusarium oxysporum, and Rhizoctonia solani, in vitro and in planta. Eleven isolates identified Pseudomonas sp. CRB numbered by CRB-3, CRB-16, CRB-17, CRB-31, CRB-44, CRB-75, CRB-80, CRB-86, CRB-102, CRB-109, and CRB-112 were affirmed to be candidates of biocontrol agents toward the soilborne fungal pathogens. Pseudomonas sp. CRB inhibited growth of the pathogenic fungi approximately 11.1-60.0% in vitro. Among of them, 7 isolates were also produced siderophore, 2 isolates produced chitinase, and 4 isolates produced hydrogen cyanide. Seed coating with the Pseudomonas sp. CRB accomplished disease suppression in planta about 14.3-100% in sterile soil condition and 5.2-52.6% in non sterile soil condition. Consistency in high performance more than 30% of disease suppression in non sterile soil condition suggested that 5 isolates i.e. CRB-16, CRB-44, CRB-86, CRB-102, and CRB-109 isolates have great promising to be developed as biocontrol agents of soilborne pathogenic fungi. |
first_indexed | 2024-12-23T23:21:38Z |
format | Article |
id | doaj.art-f0694af10c0b4fa3880d2acc2f4cfba8 |
institution | Directory Open Access Journal |
issn | 1978-3019 2086-4094 |
language | English |
last_indexed | 2024-12-23T23:21:38Z |
publishDate | 2011-06-01 |
publisher | Bogor Agricultural University |
record_format | Article |
series | Hayati Journal of Biosciences |
spelling | doaj.art-f0694af10c0b4fa3880d2acc2f4cfba82022-12-21T17:26:20ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942011-06-011825156Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic FungiANTONIUS SUWANTOYULIN LESTARIARIS TRI WAHYUDIARI SUSILOWATISURYO WIYONOPlants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas sp. as biocontrol agent should be deeply explored. The aim of this study was to screen the indigenous soybeans’ rhizobacteria Pseudomonas sp. that possessing biocontrol characters against soilborne mainly i.e. Sclerotium rolfsii, Fusarium oxysporum, and Rhizoctonia solani, in vitro and in planta. Eleven isolates identified Pseudomonas sp. CRB numbered by CRB-3, CRB-16, CRB-17, CRB-31, CRB-44, CRB-75, CRB-80, CRB-86, CRB-102, CRB-109, and CRB-112 were affirmed to be candidates of biocontrol agents toward the soilborne fungal pathogens. Pseudomonas sp. CRB inhibited growth of the pathogenic fungi approximately 11.1-60.0% in vitro. Among of them, 7 isolates were also produced siderophore, 2 isolates produced chitinase, and 4 isolates produced hydrogen cyanide. Seed coating with the Pseudomonas sp. CRB accomplished disease suppression in planta about 14.3-100% in sterile soil condition and 5.2-52.6% in non sterile soil condition. Consistency in high performance more than 30% of disease suppression in non sterile soil condition suggested that 5 isolates i.e. CRB-16, CRB-44, CRB-86, CRB-102, and CRB-109 isolates have great promising to be developed as biocontrol agents of soilborne pathogenic fungi.http://journal.ipb.ac.id/index.php/hayati/article/view/3432/2323rhizobacteriaPseudomonas sp.biocontrol traitsdisease suppressionsoybean plant |
spellingShingle | ANTONIUS SUWANTO YULIN LESTARI ARIS TRI WAHYUDI ARI SUSILOWATI SURYO WIYONO Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi Hayati Journal of Biosciences rhizobacteria Pseudomonas sp. biocontrol traits disease suppression soybean plant |
title | Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi |
title_full | Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi |
title_fullStr | Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi |
title_full_unstemmed | Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi |
title_short | Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi |
title_sort | potential pseudomonas isolated from soybean rhizosphere as biocontrol against soilborne phytopathogenic fungi |
topic | rhizobacteria Pseudomonas sp. biocontrol traits disease suppression soybean plant |
url | http://journal.ipb.ac.id/index.php/hayati/article/view/3432/2323 |
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