Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi

Plants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas...

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Main Authors: ANTONIUS SUWANTO, YULIN LESTARI, ARIS TRI WAHYUDI, ARI SUSILOWATI, SURYO WIYONO
Format: Article
Language:English
Published: Bogor Agricultural University 2011-06-01
Series:Hayati Journal of Biosciences
Subjects:
Online Access:http://journal.ipb.ac.id/index.php/hayati/article/view/3432/2323
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author ANTONIUS SUWANTO
YULIN LESTARI
ARIS TRI WAHYUDI
ARI SUSILOWATI
SURYO WIYONO
author_facet ANTONIUS SUWANTO
YULIN LESTARI
ARIS TRI WAHYUDI
ARI SUSILOWATI
SURYO WIYONO
author_sort ANTONIUS SUWANTO
collection DOAJ
description Plants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas sp. as biocontrol agent should be deeply explored. The aim of this study was to screen the indigenous soybeans’ rhizobacteria Pseudomonas sp. that possessing biocontrol characters against soilborne mainly i.e. Sclerotium rolfsii, Fusarium oxysporum, and Rhizoctonia solani, in vitro and in planta. Eleven isolates identified Pseudomonas sp. CRB numbered by CRB-3, CRB-16, CRB-17, CRB-31, CRB-44, CRB-75, CRB-80, CRB-86, CRB-102, CRB-109, and CRB-112 were affirmed to be candidates of biocontrol agents toward the soilborne fungal pathogens. Pseudomonas sp. CRB inhibited growth of the pathogenic fungi approximately 11.1-60.0% in vitro. Among of them, 7 isolates were also produced siderophore, 2 isolates produced chitinase, and 4 isolates produced hydrogen cyanide. Seed coating with the Pseudomonas sp. CRB accomplished disease suppression in planta about 14.3-100% in sterile soil condition and 5.2-52.6% in non sterile soil condition. Consistency in high performance more than 30% of disease suppression in non sterile soil condition suggested that 5 isolates i.e. CRB-16, CRB-44, CRB-86, CRB-102, and CRB-109 isolates have great promising to be developed as biocontrol agents of soilborne pathogenic fungi.
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spelling doaj.art-f0694af10c0b4fa3880d2acc2f4cfba82022-12-21T17:26:20ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942011-06-011825156Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic FungiANTONIUS SUWANTOYULIN LESTARIARIS TRI WAHYUDIARI SUSILOWATISURYO WIYONOPlants are liable to be attacked by soilborne fungal pathogens which are responsible to reduce plant growth and losses in yield. In Indonesia, indigenous soybeans’ rhizobacteria such as antifungal producing Pseudomonas sp. have not many been reported yet. Therefore, the potential of the Pseudomonas sp. as biocontrol agent should be deeply explored. The aim of this study was to screen the indigenous soybeans’ rhizobacteria Pseudomonas sp. that possessing biocontrol characters against soilborne mainly i.e. Sclerotium rolfsii, Fusarium oxysporum, and Rhizoctonia solani, in vitro and in planta. Eleven isolates identified Pseudomonas sp. CRB numbered by CRB-3, CRB-16, CRB-17, CRB-31, CRB-44, CRB-75, CRB-80, CRB-86, CRB-102, CRB-109, and CRB-112 were affirmed to be candidates of biocontrol agents toward the soilborne fungal pathogens. Pseudomonas sp. CRB inhibited growth of the pathogenic fungi approximately 11.1-60.0% in vitro. Among of them, 7 isolates were also produced siderophore, 2 isolates produced chitinase, and 4 isolates produced hydrogen cyanide. Seed coating with the Pseudomonas sp. CRB accomplished disease suppression in planta about 14.3-100% in sterile soil condition and 5.2-52.6% in non sterile soil condition. Consistency in high performance more than 30% of disease suppression in non sterile soil condition suggested that 5 isolates i.e. CRB-16, CRB-44, CRB-86, CRB-102, and CRB-109 isolates have great promising to be developed as biocontrol agents of soilborne pathogenic fungi.http://journal.ipb.ac.id/index.php/hayati/article/view/3432/2323rhizobacteriaPseudomonas sp.biocontrol traitsdisease suppressionsoybean plant
spellingShingle ANTONIUS SUWANTO
YULIN LESTARI
ARIS TRI WAHYUDI
ARI SUSILOWATI
SURYO WIYONO
Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
Hayati Journal of Biosciences
rhizobacteria
Pseudomonas sp.
biocontrol traits
disease suppression
soybean plant
title Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
title_full Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
title_fullStr Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
title_full_unstemmed Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
title_short Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol against Soilborne Phytopathogenic Fungi
title_sort potential pseudomonas isolated from soybean rhizosphere as biocontrol against soilborne phytopathogenic fungi
topic rhizobacteria
Pseudomonas sp.
biocontrol traits
disease suppression
soybean plant
url http://journal.ipb.ac.id/index.php/hayati/article/view/3432/2323
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