Tracking Eye Movements During Sleep in Mice
Eye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye mov...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2021.616760/full |
| _version_ | 1818328426436624384 |
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| author | Qingshuo Meng Xinrong Tan Chengyong Jiang Yanyu Xiong Biao Yan Jiayi Zhang |
| author_facet | Qingshuo Meng Xinrong Tan Chengyong Jiang Yanyu Xiong Biao Yan Jiayi Zhang |
| author_sort | Qingshuo Meng |
| collection | DOAJ |
| description | Eye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye movement (REM) sleep, indicating that the brain status of REM sleep likely resembles that of awake status. REM sleep in humans could be divided into phasic REM and tonic REM sleep according to the difference in eye movement frequencies. Mice are the most commonly used animal model for studying neuronal and molecular mechanisms underlying sleep. However, there was a lack of details for eye movement patterns during REM sleep, hence it remains unknown whether REM sleep can be further divided into different stages in mice. Here we developed a device combining electroencephalogram (EEG), electromyogram (EMG) as well as eye movements recording in mice to study the eye movement patterns during sleep. We implanted a magnet beneath the conjunctiva of eye and tracked eye movements using a magnetic sensor. The magnetic signals showed strong correlation with video-oculography in head-fixed mice, indicating that the magnetic signals reflect the direction and magnitude of eye movement. We also found that the magnet implanted beneath the conjunctiva exhibited good biocompatibility. Finally, we examined eye movement in sleep–wake cycle, and discriminated tonic REM and phasic REM according to the frequency of eye movements, finding that compared to tonic REM, phasic REM exhibited higher oscillation power at 0.50 Hz, and lower oscillation power at 1.50–7.25 Hz and 9.50–12.00 Hz. Our device allowed to simultaneously record EEG, EMG, and eye movements during sleep and wakefulness, providing a convenient and high temporal-spatial resolution tool for studying eye movements in sleep and other researches in mice. |
| first_indexed | 2024-12-13T12:31:59Z |
| format | Article |
| id | doaj.art-f06dcd322968432db0e8e926fcabff6d |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-13T12:31:59Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-f06dcd322968432db0e8e926fcabff6d2022-12-21T23:46:01ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2021-02-011510.3389/fnins.2021.616760616760Tracking Eye Movements During Sleep in MiceQingshuo MengXinrong TanChengyong JiangYanyu XiongBiao YanJiayi ZhangEye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye movement (REM) sleep, indicating that the brain status of REM sleep likely resembles that of awake status. REM sleep in humans could be divided into phasic REM and tonic REM sleep according to the difference in eye movement frequencies. Mice are the most commonly used animal model for studying neuronal and molecular mechanisms underlying sleep. However, there was a lack of details for eye movement patterns during REM sleep, hence it remains unknown whether REM sleep can be further divided into different stages in mice. Here we developed a device combining electroencephalogram (EEG), electromyogram (EMG) as well as eye movements recording in mice to study the eye movement patterns during sleep. We implanted a magnet beneath the conjunctiva of eye and tracked eye movements using a magnetic sensor. The magnetic signals showed strong correlation with video-oculography in head-fixed mice, indicating that the magnetic signals reflect the direction and magnitude of eye movement. We also found that the magnet implanted beneath the conjunctiva exhibited good biocompatibility. Finally, we examined eye movement in sleep–wake cycle, and discriminated tonic REM and phasic REM according to the frequency of eye movements, finding that compared to tonic REM, phasic REM exhibited higher oscillation power at 0.50 Hz, and lower oscillation power at 1.50–7.25 Hz and 9.50–12.00 Hz. Our device allowed to simultaneously record EEG, EMG, and eye movements during sleep and wakefulness, providing a convenient and high temporal-spatial resolution tool for studying eye movements in sleep and other researches in mice.https://www.frontiersin.org/articles/10.3389/fnins.2021.616760/fulleye movement trackingsleep–wake cycletonic REMphasic REMbiocompatibility |
| spellingShingle | Qingshuo Meng Xinrong Tan Chengyong Jiang Yanyu Xiong Biao Yan Jiayi Zhang Tracking Eye Movements During Sleep in Mice Frontiers in Neuroscience eye movement tracking sleep–wake cycle tonic REM phasic REM biocompatibility |
| title | Tracking Eye Movements During Sleep in Mice |
| title_full | Tracking Eye Movements During Sleep in Mice |
| title_fullStr | Tracking Eye Movements During Sleep in Mice |
| title_full_unstemmed | Tracking Eye Movements During Sleep in Mice |
| title_short | Tracking Eye Movements During Sleep in Mice |
| title_sort | tracking eye movements during sleep in mice |
| topic | eye movement tracking sleep–wake cycle tonic REM phasic REM biocompatibility |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2021.616760/full |
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