Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i>
GroEL is a chaperonin that helps other proteins fold correctly. However, alternative activities, such as acting as an insect toxin, have also been discovered. This work evaluates the chaperonin and insecticidal activity of different GroEL proteins from entomopathogenic nematodes on <i>G. mello...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/11/623 |
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| author | Abraham Rivera-Ramírez Rosalba Salgado-Morales Janette Onofre-Lemus Blanca I. García-Gómez Humberto Lanz-Mendoza Edgar Dantán-González |
| author_facet | Abraham Rivera-Ramírez Rosalba Salgado-Morales Janette Onofre-Lemus Blanca I. García-Gómez Humberto Lanz-Mendoza Edgar Dantán-González |
| author_sort | Abraham Rivera-Ramírez |
| collection | DOAJ |
| description | GroEL is a chaperonin that helps other proteins fold correctly. However, alternative activities, such as acting as an insect toxin, have also been discovered. This work evaluates the chaperonin and insecticidal activity of different GroEL proteins from entomopathogenic nematodes on <i>G. mellonella</i>. The ability to synergize with the ExoA toxin of <i>Pseudomonas aeruginosa</i> was also investigated. The GroELXn protein showed the highest insecticidal activity among the different GroELs. In addition, it was able to significantly activate the phenoloxidase system of the target insects. This could tell us about the mechanism by which it exerts its toxicity on insects. GroEL proteins can enhance the toxic activity of the ExoA toxin, which could be related to its chaperonin activity. However, there is a significant difference in the synergistic effect that is more related to its alternative activity as an insecticidal toxin. |
| first_indexed | 2024-03-09T16:25:07Z |
| format | Article |
| id | doaj.art-f06ea0b5591d4ed2b9eef50a2bc5935d |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T16:25:07Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f06ea0b5591d4ed2b9eef50a2bc5935d2023-11-24T15:09:29ZengMDPI AGToxins2072-66512023-10-01151162310.3390/toxins15110623Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i>Abraham Rivera-Ramírez0Rosalba Salgado-Morales1Janette Onofre-Lemus2Blanca I. García-Gómez3Humberto Lanz-Mendoza4Edgar Dantán-González5Center for Population Health Research, National Institute of Public Health, Cuernavaca 62100, MexicoBiotechnology Research Center, Autonomous University of the State of Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, MexicoBiotechnology Research Center, Autonomous University of the State of Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, MexicoBiotechnology Institute, National Autonomous University of Mexico, A.P. 510-3, Cuernavaca 62250, MexicoCenter for Research on Infectious Diseases, National Institute of Public Health, Cuernavaca 62100, MexicoBiotechnology Research Center, Autonomous University of the State of Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, MexicoGroEL is a chaperonin that helps other proteins fold correctly. However, alternative activities, such as acting as an insect toxin, have also been discovered. This work evaluates the chaperonin and insecticidal activity of different GroEL proteins from entomopathogenic nematodes on <i>G. mellonella</i>. The ability to synergize with the ExoA toxin of <i>Pseudomonas aeruginosa</i> was also investigated. The GroELXn protein showed the highest insecticidal activity among the different GroELs. In addition, it was able to significantly activate the phenoloxidase system of the target insects. This could tell us about the mechanism by which it exerts its toxicity on insects. GroEL proteins can enhance the toxic activity of the ExoA toxin, which could be related to its chaperonin activity. However, there is a significant difference in the synergistic effect that is more related to its alternative activity as an insecticidal toxin.https://www.mdpi.com/2072-6651/15/11/623chaperoninGroELinsecticidal<i>G. mellonella</i>synergism |
| spellingShingle | Abraham Rivera-Ramírez Rosalba Salgado-Morales Janette Onofre-Lemus Blanca I. García-Gómez Humberto Lanz-Mendoza Edgar Dantán-González Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i> Toxins chaperonin GroEL insecticidal <i>G. mellonella</i> synergism |
| title | Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i> |
| title_full | Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i> |
| title_fullStr | Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i> |
| title_full_unstemmed | Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i> |
| title_short | Evaluation and Characterization of the Insecticidal Activity and Synergistic Effects of Different GroEL Proteins from Bacteria Associated with Entomopathogenic Nematodes on <i>Galleria mellonella</i> |
| title_sort | evaluation and characterization of the insecticidal activity and synergistic effects of different groel proteins from bacteria associated with entomopathogenic nematodes on i galleria mellonella i |
| topic | chaperonin GroEL insecticidal <i>G. mellonella</i> synergism |
| url | https://www.mdpi.com/2072-6651/15/11/623 |
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