Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes
Neural regeneration is very limited in humans but extremely efficient in echinoderms. The brittle star <i>Amphiura filiformis</i> can regenerate both components of its central nervous system as well as the peripheral system, and understanding the molecular mechanisms underlying this abil...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/9/1360 |
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| author | Anna Czarkwiani Jack Taylor Paola Oliveri |
| author_facet | Anna Czarkwiani Jack Taylor Paola Oliveri |
| author_sort | Anna Czarkwiani |
| collection | DOAJ |
| description | Neural regeneration is very limited in humans but extremely efficient in echinoderms. The brittle star <i>Amphiura filiformis</i> can regenerate both components of its central nervous system as well as the peripheral system, and understanding the molecular mechanisms underlying this ability is key for evolutionary comparisons not only within the echinoderm group, but also wider within deuterostomes. Here we characterise the neural regeneration of this brittle star using a combination of immunohistochemistry, in situ hybridization and Nanostring nCounter to determine the spatial and temporal expression of evolutionary conserved neural genes. We find that key genes crucial for the embryonic development of the nervous system in sea urchins and other animals are also expressed in the regenerating nervous system of the adult brittle star in a hierarchic and spatio-temporally restricted manner. |
| first_indexed | 2024-03-10T00:40:47Z |
| format | Article |
| id | doaj.art-f07097f49a6843bea942ececfbd3c4e2 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:40:47Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-f07097f49a6843bea942ececfbd3c4e22023-11-23T15:08:13ZengMDPI AGBiology2079-77372022-09-01119136010.3390/biology11091360Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental GenesAnna Czarkwiani0Jack Taylor1Paola Oliveri2Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UKDepartment of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UKDepartment of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UKNeural regeneration is very limited in humans but extremely efficient in echinoderms. The brittle star <i>Amphiura filiformis</i> can regenerate both components of its central nervous system as well as the peripheral system, and understanding the molecular mechanisms underlying this ability is key for evolutionary comparisons not only within the echinoderm group, but also wider within deuterostomes. Here we characterise the neural regeneration of this brittle star using a combination of immunohistochemistry, in situ hybridization and Nanostring nCounter to determine the spatial and temporal expression of evolutionary conserved neural genes. We find that key genes crucial for the embryonic development of the nervous system in sea urchins and other animals are also expressed in the regenerating nervous system of the adult brittle star in a hierarchic and spatio-temporally restricted manner.https://www.mdpi.com/2079-7737/11/9/1360ophiuroidechinodermregenerationnervous systempax6elav |
| spellingShingle | Anna Czarkwiani Jack Taylor Paola Oliveri Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes Biology ophiuroid echinoderm regeneration nervous system pax6 elav |
| title | Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes |
| title_full | Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes |
| title_fullStr | Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes |
| title_full_unstemmed | Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes |
| title_short | Neurogenesis during Brittle Star Arm Regeneration Is Characterised by a Conserved Set of Key Developmental Genes |
| title_sort | neurogenesis during brittle star arm regeneration is characterised by a conserved set of key developmental genes |
| topic | ophiuroid echinoderm regeneration nervous system pax6 elav |
| url | https://www.mdpi.com/2079-7737/11/9/1360 |
| work_keys_str_mv | AT annaczarkwiani neurogenesisduringbrittlestararmregenerationischaracterisedbyaconservedsetofkeydevelopmentalgenes AT jacktaylor neurogenesisduringbrittlestararmregenerationischaracterisedbyaconservedsetofkeydevelopmentalgenes AT paolaoliveri neurogenesisduringbrittlestararmregenerationischaracterisedbyaconservedsetofkeydevelopmentalgenes |