A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study
The separation of circulating tumor cells (CTCs) from blood samples is crucial for the early diagnosis of cancer. During recent years, hybrid microfluidics platforms, consisting of both passive and active components, have been an emerging means for the label-free enrichment of circulating tumor cell...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/3/171 |
| _version_ | 1797472647982874624 |
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| author | Rana Altay Murat Kaya Yapici Ali Koşar |
| author_facet | Rana Altay Murat Kaya Yapici Ali Koşar |
| author_sort | Rana Altay |
| collection | DOAJ |
| description | The separation of circulating tumor cells (CTCs) from blood samples is crucial for the early diagnosis of cancer. During recent years, hybrid microfluidics platforms, consisting of both passive and active components, have been an emerging means for the label-free enrichment of circulating tumor cells due to their advantages such as multi-target cell processing with high efficiency and high sensitivity. In this study, spiral microchannels with different dimensions were coupled with surface acoustic waves (SAWs). Numerical simulations were conducted at different Reynolds numbers to analyze the performance of hybrid devices in the sorting and separation of CTCs from red blood cells (RBCs) and white blood cells (WBCs). Overall, in the first stage, the two-loop spiral microchannel structure allowed for the utilization of inertial forces for passive separation. In the second stage, SAWs were introduced to the device. Thus, five nodal pressure lines corresponding to the lateral position of the five outlets were generated. According to their physical properties, the cells were trapped and lined up on the corresponding nodal lines. The results showed that three different cell types (CTCs, RBCs, and WBCs) were successfully focused and collected from the different outlets of the microchannels by implementing the proposed multi-stage hybrid system. |
| first_indexed | 2024-03-09T20:04:21Z |
| format | Article |
| id | doaj.art-f073c7cee7774876a93ca090d3fed39e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:04:21Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f073c7cee7774876a93ca090d3fed39e2023-11-24T00:36:37ZengMDPI AGBiosensors2079-63742022-03-0112317110.3390/bios12030171A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical StudyRana Altay0Murat Kaya Yapici1Ali Koşar2Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, TurkeyFaculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, TurkeyFaculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, TurkeyThe separation of circulating tumor cells (CTCs) from blood samples is crucial for the early diagnosis of cancer. During recent years, hybrid microfluidics platforms, consisting of both passive and active components, have been an emerging means for the label-free enrichment of circulating tumor cells due to their advantages such as multi-target cell processing with high efficiency and high sensitivity. In this study, spiral microchannels with different dimensions were coupled with surface acoustic waves (SAWs). Numerical simulations were conducted at different Reynolds numbers to analyze the performance of hybrid devices in the sorting and separation of CTCs from red blood cells (RBCs) and white blood cells (WBCs). Overall, in the first stage, the two-loop spiral microchannel structure allowed for the utilization of inertial forces for passive separation. In the second stage, SAWs were introduced to the device. Thus, five nodal pressure lines corresponding to the lateral position of the five outlets were generated. According to their physical properties, the cells were trapped and lined up on the corresponding nodal lines. The results showed that three different cell types (CTCs, RBCs, and WBCs) were successfully focused and collected from the different outlets of the microchannels by implementing the proposed multi-stage hybrid system.https://www.mdpi.com/2079-6374/12/3/171cell separationcell sortingcirculating tumor cells (CTCs)inertial microfluidicssurface acoustic wave (SAW)hybrid separation |
| spellingShingle | Rana Altay Murat Kaya Yapici Ali Koşar A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study Biosensors cell separation cell sorting circulating tumor cells (CTCs) inertial microfluidics surface acoustic wave (SAW) hybrid separation |
| title | A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study |
| title_full | A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study |
| title_fullStr | A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study |
| title_full_unstemmed | A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study |
| title_short | A Hybrid Spiral Microfluidic Platform Coupled with Surface Acoustic Waves for Circulating Tumor Cell Sorting and Separation: A Numerical Study |
| title_sort | hybrid spiral microfluidic platform coupled with surface acoustic waves for circulating tumor cell sorting and separation a numerical study |
| topic | cell separation cell sorting circulating tumor cells (CTCs) inertial microfluidics surface acoustic wave (SAW) hybrid separation |
| url | https://www.mdpi.com/2079-6374/12/3/171 |
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