Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo
Abstract Background Targeted contrast nanoparticles for breast tumor imaging facilitates early detection and improves treatment efficacy of breast cancer. This manuscript reports the development of an epidermal growth factor receptor-2 (HER-2) specific, bi-modal, dendrimer conjugate to enhance compu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-020-00695-2 |
| _version_ | 1798005382756433920 |
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| author | Jesse S. Chen Jingwen Chen Somnath Bhattacharjee Zhengyi Cao Han Wang Scott D. Swanson Hong Zong James R. Baker Su He Wang |
| author_facet | Jesse S. Chen Jingwen Chen Somnath Bhattacharjee Zhengyi Cao Han Wang Scott D. Swanson Hong Zong James R. Baker Su He Wang |
| author_sort | Jesse S. Chen |
| collection | DOAJ |
| description | Abstract Background Targeted contrast nanoparticles for breast tumor imaging facilitates early detection and improves treatment efficacy of breast cancer. This manuscript reports the development of an epidermal growth factor receptor-2 (HER-2) specific, bi-modal, dendrimer conjugate to enhance computed tomography (CT) and magnetic resonance imaging (MRI) of HER-2-positive breast cancer. This material employs generation 5 poly(amidoamine) dendrimers, encapsulated gold nanoparticles, chelated gadolinium, and anti-human HER-2 antibody to produce the nanoparticle contrast agent. Results Testing in two mouse tumor models confirms this contrast agent’s ability to image HER-2 positive tumors. Intravenous injection of this nanoparticle in mice bearing HER-2 positive mammary tumors significantly enhances MRI signal intensity by ~ 20% and improves CT resolution and contrast by two-fold. Results by flow cytometry and confocal microscopy validate the specific targeting of the conjugate and its internalization in human HER-2 positive cells. Conclusion These results demonstrate that this nanoparticle conjugate can efficiently target and image HER-2 positive tumors in vivo and provide a basis for the development of this diagnostic tool for early detection, metastatic assessment and therapeutic monitoring of HER-2 positive cancers. |
| first_indexed | 2024-04-11T12:38:19Z |
| format | Article |
| id | doaj.art-f074dc2b546a49d9ab2ad4961b315374 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T12:38:19Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-f074dc2b546a49d9ab2ad4961b3153742022-12-22T04:23:34ZengBMCJournal of Nanobiotechnology1477-31552020-09-011811910.1186/s12951-020-00695-2Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivoJesse S. Chen0Jingwen Chen1Somnath Bhattacharjee2Zhengyi Cao3Han Wang4Scott D. Swanson5Hong Zong6James R. Baker7Su He Wang8Department of Internal Medicine, Division of Allergy, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of MichiganDepartment of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Internal Medicine, Division of Allergy, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of MichiganDepartment of Internal Medicine, Division of Allergy, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of MichiganDepartment of Radiology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Radiology, University of MichiganDepartment of Internal Medicine, Division of Allergy, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of MichiganDepartment of Internal Medicine, Division of Allergy, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of MichiganDepartment of Internal Medicine, Division of Allergy, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of MichiganAbstract Background Targeted contrast nanoparticles for breast tumor imaging facilitates early detection and improves treatment efficacy of breast cancer. This manuscript reports the development of an epidermal growth factor receptor-2 (HER-2) specific, bi-modal, dendrimer conjugate to enhance computed tomography (CT) and magnetic resonance imaging (MRI) of HER-2-positive breast cancer. This material employs generation 5 poly(amidoamine) dendrimers, encapsulated gold nanoparticles, chelated gadolinium, and anti-human HER-2 antibody to produce the nanoparticle contrast agent. Results Testing in two mouse tumor models confirms this contrast agent’s ability to image HER-2 positive tumors. Intravenous injection of this nanoparticle in mice bearing HER-2 positive mammary tumors significantly enhances MRI signal intensity by ~ 20% and improves CT resolution and contrast by two-fold. Results by flow cytometry and confocal microscopy validate the specific targeting of the conjugate and its internalization in human HER-2 positive cells. Conclusion These results demonstrate that this nanoparticle conjugate can efficiently target and image HER-2 positive tumors in vivo and provide a basis for the development of this diagnostic tool for early detection, metastatic assessment and therapeutic monitoring of HER-2 positive cancers.http://link.springer.com/article/10.1186/s12951-020-00695-2TargetingNanoparticlesBi-modal imagingComputerized tomographyMagnetic resonance imagingHER-2 |
| spellingShingle | Jesse S. Chen Jingwen Chen Somnath Bhattacharjee Zhengyi Cao Han Wang Scott D. Swanson Hong Zong James R. Baker Su He Wang Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo Journal of Nanobiotechnology Targeting Nanoparticles Bi-modal imaging Computerized tomography Magnetic resonance imaging HER-2 |
| title | Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo |
| title_full | Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo |
| title_fullStr | Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo |
| title_full_unstemmed | Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo |
| title_short | Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo |
| title_sort | functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo |
| topic | Targeting Nanoparticles Bi-modal imaging Computerized tomography Magnetic resonance imaging HER-2 |
| url | http://link.springer.com/article/10.1186/s12951-020-00695-2 |
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