Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats
Objective(s): Ischemia/reperfusion (I/R) is a leading cause of myocardial infarction (MI) injury, contributing to excess injury to cardiac tissues involved in inflammation, apoptosis, and oxidative stress. The present study was conducted to examine the effects of combined thymoquinone (TQ) with isch...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_18395_5776f1d2a749f8e59c3a909cd9b1319d.pdf |
| _version_ | 1818975933976018944 |
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| author | Junchuan Ran Huanglin Xu Wenyuan Li |
| author_facet | Junchuan Ran Huanglin Xu Wenyuan Li |
| author_sort | Junchuan Ran |
| collection | DOAJ |
| description | Objective(s): Ischemia/reperfusion (I/R) is a leading cause of myocardial infarction (MI) injury, contributing to excess injury to cardiac tissues involved in inflammation, apoptosis, and oxidative stress. The present study was conducted to examine the effects of combined thymoquinone (TQ) with ischemic postconditioning (IPostC) therapy on apoptosis and inflammation due to I/R injury in diabetic rat hearts. Materials and Methods: A single dose injection of streptozotocin (STZ; 60 mg/kg) was administered to thirty-two Wistar male rats to induce diabetes. Hearts were fixed on a Langendorff setting and exposed to a 30 min regional ischemia subsequently to 60 min reperfusion. IPostC was induced at the onset of reperfusion by 3 cycles of 30 sec R/I. ELISA, Western blotting assay, and TUNEL staining were applied to assess the cardioprotective effect of IPostC and TQ against I/R injury in diabetic and non-diabetic rats.Results: Administration of TQ alone in non-diabetic isolated hearts significantly diminished CK-MB, TNF-α, IL-1β, and apoptosis and enhanced p-GSK-3β and Bcl-2 (p <0.05). Following administration of TQ, the cardioprotective effects of IPostC by elevating p-GSK-3β and Bcl-2 and alleviating apoptosis and inflammation were reestablished compared with non-IPostC diabetic hearts. Conclusion: These results provide substantial evidence that co-administration of TQ plus IPostC can exert cardioprotective effects on diabetic myocardium during I/R damage by attenuating the inflammatory response and apoptosis. |
| first_indexed | 2024-12-20T16:03:50Z |
| format | Article |
| id | doaj.art-f076eef9f6e547e491cb409f2693286c |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-20T16:03:50Z |
| publishDate | 2021-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-f076eef9f6e547e491cb409f2693286c2022-12-21T19:34:13ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-07-0124789289910.22038/ijbms.2021.47670.1098118395Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic ratsJunchuan Ran0Huanglin Xu1Wenyuan Li2Department of Cardiology, Gansu Gem Flower Hospital, Lanzhou, Gansu, 730060, ChinaDepartment of Cardiology, Xigu People’s Hospital,Lanzhou, Gansu, 730060, ChinaDepartment of Cardiology, Gansu Gem Flower Hospital, Lanzhou, Gansu, 730060, ChinaObjective(s): Ischemia/reperfusion (I/R) is a leading cause of myocardial infarction (MI) injury, contributing to excess injury to cardiac tissues involved in inflammation, apoptosis, and oxidative stress. The present study was conducted to examine the effects of combined thymoquinone (TQ) with ischemic postconditioning (IPostC) therapy on apoptosis and inflammation due to I/R injury in diabetic rat hearts. Materials and Methods: A single dose injection of streptozotocin (STZ; 60 mg/kg) was administered to thirty-two Wistar male rats to induce diabetes. Hearts were fixed on a Langendorff setting and exposed to a 30 min regional ischemia subsequently to 60 min reperfusion. IPostC was induced at the onset of reperfusion by 3 cycles of 30 sec R/I. ELISA, Western blotting assay, and TUNEL staining were applied to assess the cardioprotective effect of IPostC and TQ against I/R injury in diabetic and non-diabetic rats.Results: Administration of TQ alone in non-diabetic isolated hearts significantly diminished CK-MB, TNF-α, IL-1β, and apoptosis and enhanced p-GSK-3β and Bcl-2 (p <0.05). Following administration of TQ, the cardioprotective effects of IPostC by elevating p-GSK-3β and Bcl-2 and alleviating apoptosis and inflammation were reestablished compared with non-IPostC diabetic hearts. Conclusion: These results provide substantial evidence that co-administration of TQ plus IPostC can exert cardioprotective effects on diabetic myocardium during I/R damage by attenuating the inflammatory response and apoptosis.https://ijbms.mums.ac.ir/article_18395_5776f1d2a749f8e59c3a909cd9b1319d.pdfapoptosisdiabetesinflammationischemic postconditioningpolyphenols |
| spellingShingle | Junchuan Ran Huanglin Xu Wenyuan Li Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats Iranian Journal of Basic Medical Sciences apoptosis diabetes inflammation ischemic postconditioning polyphenols |
| title | Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats |
| title_full | Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats |
| title_fullStr | Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats |
| title_full_unstemmed | Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats |
| title_short | Cardioprotective effects of co-administration of thymoquinone and ischemic postconditioning in diabetic rats |
| title_sort | cardioprotective effects of co administration of thymoquinone and ischemic postconditioning in diabetic rats |
| topic | apoptosis diabetes inflammation ischemic postconditioning polyphenols |
| url | https://ijbms.mums.ac.ir/article_18395_5776f1d2a749f8e59c3a909cd9b1319d.pdf |
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