High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2
Summary: Anti-virulence therapies are under active investigation as antibiotic alternatives; however, their identification from large-scale chemical libraries poses a unique challenge. The dispensability of virulence factors for growth in vitro precludes conventional, optical density-based screening...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-09-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300447 |
| _version_ | 1818341343287574528 |
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| author | Caressa N. Tsai Brian K. Coombes |
| author_facet | Caressa N. Tsai Brian K. Coombes |
| author_sort | Caressa N. Tsai |
| collection | DOAJ |
| description | Summary: Anti-virulence therapies are under active investigation as antibiotic alternatives; however, their identification from large-scale chemical libraries poses a unique challenge. The dispensability of virulence factors for growth in vitro precludes conventional, optical density-based screening methods. Here, we provide a protocol for high-throughput screening with a cell-based, promoter reporter platform. We describe the use of this method for the identification of anti-SPI-2 inhibitors specific to Salmonella Typhimurium, which may be modified to investigate other virulence factors.For complete details on the use and execution of this protocol, please refer to Tsai et al. (2020). |
| first_indexed | 2024-12-13T15:57:17Z |
| format | Article |
| id | doaj.art-f0778725ec634d33b30234d0a73043e8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T15:57:17Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f0778725ec634d33b30234d0a73043e82022-12-21T23:39:17ZengElsevierSTAR Protocols2666-16672020-09-0112100057High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2Caressa N. Tsai0Brian K. Coombes1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4L8, Canada; Corresponding authorDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4L8, Canada; Corresponding authorSummary: Anti-virulence therapies are under active investigation as antibiotic alternatives; however, their identification from large-scale chemical libraries poses a unique challenge. The dispensability of virulence factors for growth in vitro precludes conventional, optical density-based screening methods. Here, we provide a protocol for high-throughput screening with a cell-based, promoter reporter platform. We describe the use of this method for the identification of anti-SPI-2 inhibitors specific to Salmonella Typhimurium, which may be modified to investigate other virulence factors.For complete details on the use and execution of this protocol, please refer to Tsai et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720300447 |
| spellingShingle | Caressa N. Tsai Brian K. Coombes High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2 STAR Protocols |
| title | High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2 |
| title_full | High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2 |
| title_fullStr | High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2 |
| title_full_unstemmed | High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2 |
| title_short | High-Throughput Chemical Screening for Inhibitors of Salmonella Pathogenicity Island 2 |
| title_sort | high throughput chemical screening for inhibitors of salmonella pathogenicity island 2 |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300447 |
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