Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report
The occasion is when there is discordance between the results of the cell-based and virtual crossmatch and requires further workup before considering a patient for renal transplant. A case of the positive single-antigen bead (SAB) with donor-specific antibodies (DSA) against HLA-A*33:01 with negativ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Indian Journal of Transplantation |
| Subjects: | |
| Online Access: | http://www.ijtonline.in/article.asp?issn=2212-0017;year=2022;volume=16;issue=4;spage=425;epage=427;aulast=Mishra |
| _version_ | 1797955031345922048 |
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| author | Vikash Chandra Mishra Dinesh Chandra Archana Anthwal Amit Kr Bhardwaj Vimarsh Raina |
| author_facet | Vikash Chandra Mishra Dinesh Chandra Archana Anthwal Amit Kr Bhardwaj Vimarsh Raina |
| author_sort | Vikash Chandra Mishra |
| collection | DOAJ |
| description | The occasion is when there is discordance between the results of the cell-based and virtual crossmatch and requires further workup before considering a patient for renal transplant. A case of the positive single-antigen bead (SAB) with donor-specific antibodies (DSA) against HLA-A*33:01 with negative complement-dependent cytotoxicity crossmatch and flow crossmatch was identified. The acid treatments of the beads were done to denature the antigen to understand the nature of identified DSA. There was variation in the Mean fluorescence intensity (MFI) observed in between patient sera with acid-treated beads (significantly increased) in contrast to regularly untreated SAB. This indicated additional antigens become available by the denaturation process. Hence, before making a final decision about the transplant in cases of ambiguity, SAB results should be interpreted in light of other compatibility results. |
| first_indexed | 2024-04-10T23:26:53Z |
| format | Article |
| id | doaj.art-f0783a377c6b4bc493b40088c08c4db1 |
| institution | Directory Open Access Journal |
| issn | 2212-0017 2212-0025 |
| language | English |
| last_indexed | 2024-04-10T23:26:53Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Transplantation |
| spelling | doaj.art-f0783a377c6b4bc493b40088c08c4db12023-01-12T12:11:20ZengWolters Kluwer Medknow PublicationsIndian Journal of Transplantation2212-00172212-00252022-01-0116442542710.4103/ijot.ijot_73_21Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case reportVikash Chandra MishraDinesh ChandraArchana AnthwalAmit Kr BhardwajVimarsh RainaThe occasion is when there is discordance between the results of the cell-based and virtual crossmatch and requires further workup before considering a patient for renal transplant. A case of the positive single-antigen bead (SAB) with donor-specific antibodies (DSA) against HLA-A*33:01 with negative complement-dependent cytotoxicity crossmatch and flow crossmatch was identified. The acid treatments of the beads were done to denature the antigen to understand the nature of identified DSA. There was variation in the Mean fluorescence intensity (MFI) observed in between patient sera with acid-treated beads (significantly increased) in contrast to regularly untreated SAB. This indicated additional antigens become available by the denaturation process. Hence, before making a final decision about the transplant in cases of ambiguity, SAB results should be interpreted in light of other compatibility results.http://www.ijtonline.in/article.asp?issn=2212-0017;year=2022;volume=16;issue=4;spage=425;epage=427;aulast=Mishradenature antigendonor-specific antibodiesfalse positiverenal transplantsingle-antigen bead |
| spellingShingle | Vikash Chandra Mishra Dinesh Chandra Archana Anthwal Amit Kr Bhardwaj Vimarsh Raina Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report Indian Journal of Transplantation denature antigen donor-specific antibodies false positive renal transplant single-antigen bead |
| title | Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report |
| title_full | Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report |
| title_fullStr | Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report |
| title_full_unstemmed | Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report |
| title_short | Positive single-antigen bead assay with negative flow crossmatch in a renal transplant - A case report |
| title_sort | positive single antigen bead assay with negative flow crossmatch in a renal transplant a case report |
| topic | denature antigen donor-specific antibodies false positive renal transplant single-antigen bead |
| url | http://www.ijtonline.in/article.asp?issn=2212-0017;year=2022;volume=16;issue=4;spage=425;epage=427;aulast=Mishra |
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