Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters
TBX5 and NKX2-5 are two important transcription factors regulating cardiomyocytes commitment. In this study, we generated a dual fluorescent reporter cell line based on H9 human embryonic stem cells (hESCs) and called it H9-NKX2.5GFP/TBX5Td-T2A-18, which could be short for T2A-18. When T2A-18 was in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123000016 |
| _version_ | 1797897336351883264 |
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| author | Zhazheng He Hao Sun Tianyi Yu Yanhan Yang Xiaoqian Zhang Yang Yang |
| author_facet | Zhazheng He Hao Sun Tianyi Yu Yanhan Yang Xiaoqian Zhang Yang Yang |
| author_sort | Zhazheng He |
| collection | DOAJ |
| description | TBX5 and NKX2-5 are two important transcription factors regulating cardiomyocytes commitment. In this study, we generated a dual fluorescent reporter cell line based on H9 human embryonic stem cells (hESCs) and called it H9-NKX2.5GFP/TBX5Td-T2A-18, which could be short for T2A-18. When T2A-18 was induced towards a cardiomyocyte fate, EGFP and Tdtomato fluorescences were observed, indicating the expression of NKX2.5 and TBX5, respectively. Meanwhile, T2A had normal karyotype and could maintain main characteristics of wildtype H9. Therefore, T2A-18 could be used as a tool to help us study the mechanism of cardiomyocytes specification from hESCs. |
| first_indexed | 2024-04-10T07:55:54Z |
| format | Article |
| id | doaj.art-f079da162c964f86b3cb1a6f317d379e |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-10T07:55:54Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-f079da162c964f86b3cb1a6f317d379e2023-02-23T04:30:50ZengElsevierStem Cell Research1873-50612023-03-0167103015Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reportersZhazheng He0Hao Sun1Tianyi Yu2Yanhan Yang3Xiaoqian Zhang4Yang Yang5State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, ChinaDepartment of Gynecology and Obstetrics, Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China; Corresponding authors at: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China.State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China; Corresponding authors at: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China.TBX5 and NKX2-5 are two important transcription factors regulating cardiomyocytes commitment. In this study, we generated a dual fluorescent reporter cell line based on H9 human embryonic stem cells (hESCs) and called it H9-NKX2.5GFP/TBX5Td-T2A-18, which could be short for T2A-18. When T2A-18 was induced towards a cardiomyocyte fate, EGFP and Tdtomato fluorescences were observed, indicating the expression of NKX2.5 and TBX5, respectively. Meanwhile, T2A had normal karyotype and could maintain main characteristics of wildtype H9. Therefore, T2A-18 could be used as a tool to help us study the mechanism of cardiomyocytes specification from hESCs.http://www.sciencedirect.com/science/article/pii/S1873506123000016 |
| spellingShingle | Zhazheng He Hao Sun Tianyi Yu Yanhan Yang Xiaoqian Zhang Yang Yang Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters Stem Cell Research |
| title | Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters |
| title_full | Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters |
| title_fullStr | Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters |
| title_full_unstemmed | Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters |
| title_short | Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters |
| title_sort | generation of a human embryonic stem cell line sklrme004 a carrying nkx2 5 egfp and tbx5 tdtomato dual fluorescent reporters |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123000016 |
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