Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways
Background/Aims: Cadmium (Cd) induces apoptosis in different kinds of cells, including osteoblasts, both in vivo and in vitro. However, little is known about the mechanisms by which Cd induces apoptosis. Methods: In the present study, we used the human osteosarcoma cell line MG63, which has characte...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2015-05-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/430127 |
| _version_ | 1819098261911240704 |
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| author | Kong-He Hu Wen-Xue Li Min-Ying Sun She-Bing Zhang Cai-Xia Fan Qiang Wu Wei Zhu Xin Xu |
| author_facet | Kong-He Hu Wen-Xue Li Min-Ying Sun She-Bing Zhang Cai-Xia Fan Qiang Wu Wei Zhu Xin Xu |
| author_sort | Kong-He Hu |
| collection | DOAJ |
| description | Background/Aims: Cadmium (Cd) induces apoptosis in different kinds of cells, including osteoblasts, both in vivo and in vitro. However, little is known about the mechanisms by which Cd induces apoptosis. Methods: In the present study, we used the human osteosarcoma cell line MG63, which has characteristics similar to human osteoblasts, as an in vitro model to determine the cellular mechanisms by which Cd induces apoptosis. Results: We found that short-term exposure to CdCl2 induced apoptosis in MG63 cells. Furthermore, the incubation of cells with CdCl2 significantly increased the level of phosphorylated p38MAPK and significantly decreased the phosphorylation of ERK1/2 in a concentration-dependent manner. Additionally, the inhibition of the phosphorylation of p38 MAPK by SB202190 protected MG63 cells from Cd-induced apoptosis. The incubation of MG63 cells with the ERK1/2 inhibitor PD98059 significantly increased apoptosis in MG63 cells. CdCl2 also significantly increased the intracellular levels of ROS. N-acetylcysteine (NAC) significantly reduced ROS levels and reversed the effects of CdCl2 on MAPK signaling. Conclusion: Our results suggested that Cd induced apoptosis in MG63 cells by increasing ROS, activation of p38 MAPK and inhibition of ERK1/2 pathways. |
| first_indexed | 2024-12-22T00:28:11Z |
| format | Article |
| id | doaj.art-f07b68fa1f8b47e38483b0d5bf93af7d |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T00:28:11Z |
| publishDate | 2015-05-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-f07b68fa1f8b47e38483b0d5bf93af7d2022-12-21T18:45:00ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-05-0136264265410.1159/000430127430127Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 PathwaysKong-He HuWen-Xue LiMin-Ying SunShe-Bing ZhangCai-Xia FanQiang WuWei ZhuXin XuBackground/Aims: Cadmium (Cd) induces apoptosis in different kinds of cells, including osteoblasts, both in vivo and in vitro. However, little is known about the mechanisms by which Cd induces apoptosis. Methods: In the present study, we used the human osteosarcoma cell line MG63, which has characteristics similar to human osteoblasts, as an in vitro model to determine the cellular mechanisms by which Cd induces apoptosis. Results: We found that short-term exposure to CdCl2 induced apoptosis in MG63 cells. Furthermore, the incubation of cells with CdCl2 significantly increased the level of phosphorylated p38MAPK and significantly decreased the phosphorylation of ERK1/2 in a concentration-dependent manner. Additionally, the inhibition of the phosphorylation of p38 MAPK by SB202190 protected MG63 cells from Cd-induced apoptosis. The incubation of MG63 cells with the ERK1/2 inhibitor PD98059 significantly increased apoptosis in MG63 cells. CdCl2 also significantly increased the intracellular levels of ROS. N-acetylcysteine (NAC) significantly reduced ROS levels and reversed the effects of CdCl2 on MAPK signaling. Conclusion: Our results suggested that Cd induced apoptosis in MG63 cells by increasing ROS, activation of p38 MAPK and inhibition of ERK1/2 pathways.http://www.karger.com/Article/FullText/430127p38MAPKERK1/2Reactive oxygen species (ROS)ApoptosisMG63 cellCadmium |
| spellingShingle | Kong-He Hu Wen-Xue Li Min-Ying Sun She-Bing Zhang Cai-Xia Fan Qiang Wu Wei Zhu Xin Xu Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways Cellular Physiology and Biochemistry p38MAPK ERK1/2 Reactive oxygen species (ROS) Apoptosis MG63 cell Cadmium |
| title | Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways |
| title_full | Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways |
| title_fullStr | Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways |
| title_full_unstemmed | Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways |
| title_short | Cadmium Induced Apoptosis in MG63 Cells by Increasing ROS, Activation of p38 MAPK and Inhibition of ERK 1/2 Pathways |
| title_sort | cadmium induced apoptosis in mg63 cells by increasing ros activation of p38 mapk and inhibition of erk 1 2 pathways |
| topic | p38MAPK ERK1/2 Reactive oxygen species (ROS) Apoptosis MG63 cell Cadmium |
| url | http://www.karger.com/Article/FullText/430127 |
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