Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran
Objectives: Urinary tract infection (UTI) is a common complication during pregnancy that can have severe consequences for both the mother and the fetus. ESBL-producing strains of E. coli present a significant challenge in terms of treatment during pregnancy. This study aims to identify pregnant wome...
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| Format: | Article |
| Language: | English |
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Kashan University of Medical Sciences
2024-04-01
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| Series: | International Archives of Health Sciences |
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| Online Access: | http://iahsj.kaums.ac.ir/article_189195_05515d145533a96267dfaf1deeb2a35d.pdf |
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| author | Zohreh Tabassi Zahra Vahedpoor-fard Hossein Sedaghat Vajihe Rouhi Mohammadreza Rahimi Somayeh Gharehhssanlou |
| author_facet | Zohreh Tabassi Zahra Vahedpoor-fard Hossein Sedaghat Vajihe Rouhi Mohammadreza Rahimi Somayeh Gharehhssanlou |
| author_sort | Zohreh Tabassi |
| collection | DOAJ |
| description | Objectives: Urinary tract infection (UTI) is a common complication during pregnancy that can have severe consequences for both the mother and the fetus. ESBL-producing strains of E. coli present a significant challenge in terms of treatment during pregnancy. This study aims to identify pregnant women carrying beta-lactamase-producing E. coli and assess the antibiotic resistance patterns of these isolates.Methods: This cross-sectional study investigated the presence of broad-spectrum beta-lactamase-producing E. coli isolates in pregnant women attending medical centers affiliated with Kashan University of Medical Sciences. Vaginal colonization, associated complications, and antibiotic resistance profiles were assessed. The antibiogram test using the disc diffusion method was employed to determine colonization and antibiotic susceptibility, while PCR testing was used to detect genes associated with beta-lactamase production.Results: Among the 175 samples collected, 51 samples tested positive for E. coli, with 15 of these samples containing ESBL-producing isolates. A significant correlation was observed between the frequency distribution of E. coli isolates and factors such as age, history of urinary tract infections, body mass index, and previous abortions in pregnant women. Among the 15 ESBL-producing E. coli isolates, the blaSHV, blaTEM, and blaCTX-M resistance genes were detected in 12 (80%), 9 (60%), and 1 (6.66%) isolates, respectively.Conclusion: The prevalence of ESBL-producing E. coli in pregnant women underscores the urgent need for public health interventions to combat antibiotic resistance. |
| first_indexed | 2024-04-24T12:48:29Z |
| format | Article |
| id | doaj.art-f07c0d8fe83d4b6ba3735a773821f8b7 |
| institution | Directory Open Access Journal |
| issn | 2383-2568 |
| language | English |
| last_indexed | 2024-04-24T12:48:29Z |
| publishDate | 2024-04-01 |
| publisher | Kashan University of Medical Sciences |
| record_format | Article |
| series | International Archives of Health Sciences |
| spelling | doaj.art-f07c0d8fe83d4b6ba3735a773821f8b72024-04-06T16:38:37ZengKashan University of Medical SciencesInternational Archives of Health Sciences2383-25682024-04-0111212813410.48307/iahsj.2024.417607.1066189195Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in IranZohreh Tabassi0Zahra Vahedpoor-fard1Hossein Sedaghat2Vajihe Rouhi3Mohammadreza Rahimi4Somayeh Gharehhssanlou5Department of Gynecology and Obstetrics, School of Medicine, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, IranDepartment of Gynecology and Obstetrics, School of Medicine, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, IranDepartment of Microbiology and Immunology, Faculty of Medicine, Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, IranSocial Determinants of Health (SDH) Research Center, Kashan University of Medical Sciences, Kashan, IranDepartment of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, IranDepartment of Gynecology and Obstetrics, School of Medicine, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, IranObjectives: Urinary tract infection (UTI) is a common complication during pregnancy that can have severe consequences for both the mother and the fetus. ESBL-producing strains of E. coli present a significant challenge in terms of treatment during pregnancy. This study aims to identify pregnant women carrying beta-lactamase-producing E. coli and assess the antibiotic resistance patterns of these isolates.Methods: This cross-sectional study investigated the presence of broad-spectrum beta-lactamase-producing E. coli isolates in pregnant women attending medical centers affiliated with Kashan University of Medical Sciences. Vaginal colonization, associated complications, and antibiotic resistance profiles were assessed. The antibiogram test using the disc diffusion method was employed to determine colonization and antibiotic susceptibility, while PCR testing was used to detect genes associated with beta-lactamase production.Results: Among the 175 samples collected, 51 samples tested positive for E. coli, with 15 of these samples containing ESBL-producing isolates. A significant correlation was observed between the frequency distribution of E. coli isolates and factors such as age, history of urinary tract infections, body mass index, and previous abortions in pregnant women. Among the 15 ESBL-producing E. coli isolates, the blaSHV, blaTEM, and blaCTX-M resistance genes were detected in 12 (80%), 9 (60%), and 1 (6.66%) isolates, respectively.Conclusion: The prevalence of ESBL-producing E. coli in pregnant women underscores the urgent need for public health interventions to combat antibiotic resistance.http://iahsj.kaums.ac.ir/article_189195_05515d145533a96267dfaf1deeb2a35d.pdfesbl-e.colipregnancyantibiotic resistance |
| spellingShingle | Zohreh Tabassi Zahra Vahedpoor-fard Hossein Sedaghat Vajihe Rouhi Mohammadreza Rahimi Somayeh Gharehhssanlou Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran International Archives of Health Sciences esbl-e.coli pregnancy antibiotic resistance |
| title | Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran |
| title_full | Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran |
| title_fullStr | Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran |
| title_full_unstemmed | Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran |
| title_short | Examination of broad-spectrum beta-lactamase-producing E. coli vaginal colonization and antibiotic resistance in pregnant women: A pilot study in Iran |
| title_sort | examination of broad spectrum beta lactamase producing e coli vaginal colonization and antibiotic resistance in pregnant women a pilot study in iran |
| topic | esbl-e.coli pregnancy antibiotic resistance |
| url | http://iahsj.kaums.ac.ir/article_189195_05515d145533a96267dfaf1deeb2a35d.pdf |
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