Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-10-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/10/2877 |
| _version_ | 1818957864511733760 |
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| author | Om Parkash Rafidah Hanim Shueb |
| author_facet | Om Parkash Rafidah Hanim Shueb |
| author_sort | Om Parkash |
| collection | DOAJ |
| description | Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed. |
| first_indexed | 2024-12-20T11:16:37Z |
| format | Article |
| id | doaj.art-f080bda0c49e49d883f84b146cc95823 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-20T11:16:37Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f080bda0c49e49d883f84b146cc958232022-12-21T19:42:36ZengMDPI AGViruses1999-49152015-10-017105410542710.3390/v7102877v7102877Diagnosis of Dengue Infection Using Conventional and Biosensor Based TechniquesOm Parkash0Rafidah Hanim Shueb1Department of Medical Microbiology and Parasitology, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, MalaysiaDepartment of Medical Microbiology and Parasitology, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, MalaysiaDengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed.http://www.mdpi.com/1999-4915/7/10/2877denguediagnosisdetectionbiosensorrt-PCRdengue specific IgMNS1viral isolation |
| spellingShingle | Om Parkash Rafidah Hanim Shueb Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques Viruses dengue diagnosis detection biosensor rt-PCR dengue specific IgM NS1 viral isolation |
| title | Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques |
| title_full | Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques |
| title_fullStr | Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques |
| title_full_unstemmed | Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques |
| title_short | Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques |
| title_sort | diagnosis of dengue infection using conventional and biosensor based techniques |
| topic | dengue diagnosis detection biosensor rt-PCR dengue specific IgM NS1 viral isolation |
| url | http://www.mdpi.com/1999-4915/7/10/2877 |
| work_keys_str_mv | AT omparkash diagnosisofdengueinfectionusingconventionalandbiosensorbasedtechniques AT rafidahhanimshueb diagnosisofdengueinfectionusingconventionalandbiosensorbasedtechniques |