Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling
Abstract Early life conditions may influence the gut microbiome and contribute to health status in adulthood. We examined the impact of maternal obesity and/or obesogenic diet on the gut microbiome of mothers (at weaning) and their adult offspring, along with the effects of leptin supplementation du...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
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| Series: | Food Frontiers |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fft2.322 |
| _version_ | 1797359939005448192 |
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| author | Catalina A. Pomar Tomeu Viver Ana Valle Juan F Gago Pedro Castillo Mariona Palou Andreu Palou Ramon Rossello‐Mora Catalina Picó |
| author_facet | Catalina A. Pomar Tomeu Viver Ana Valle Juan F Gago Pedro Castillo Mariona Palou Andreu Palou Ramon Rossello‐Mora Catalina Picó |
| author_sort | Catalina A. Pomar |
| collection | DOAJ |
| description | Abstract Early life conditions may influence the gut microbiome and contribute to health status in adulthood. We examined the impact of maternal obesity and/or obesogenic diet on the gut microbiome of mothers (at weaning) and their adult offspring, along with the effects of leptin supplementation during suckling. Three groups of rats were studied: control (C) dams, fed with standard diet (SD); western diet (WD) dams, fed with WD (a high‐fat, high‐sucrose diet) before gestation and during gestation and lactation; and reversion (REV) dams, fed as WD‐dams but with SD during lactation. Offspring were supplemented throughout suckling with leptin/vehicle and weaned on an SD. WD‐dams showed lower microbial diversity and an altered microbiome compared to C‐ and REV‐dams, widely normalized by diet improvement during lactation. WD‐ and REV‐offspring (males) displayed lower microbiome diversity and greater dominance compared to C‐offspring. Taxonomic group differences (Proteobacteria phylum and Bacteroides, Parasutterella, and Phocea genera) were observed in WD‐ and REV‐offspring. WD‐offspring, but not REV‐offspring, had a greater abundance of the phylum Firmicutes and Lachnospiraceae family and a lower abundance of the family Tannerellaceae compared to C‐offspring. Leptin supplementation led to decreased abundance of the phylum Actinobacteria (family Eggerthellaceae and genera Enterorhabdus and Adlercreutzia (females)), and the genera Lachnospiraceae UCG‐008 group, Roseburia and Limosilactobacillus, and increased abundance of the genus Dellaglioa, compared to vehicle‐treated groups. Therefore, maternal consumption of an obesogenic diet during the perinatal period results in microbiome changes in dams, alleviated by dietary improvement during lactation. Maternal conditions and leptin supplementation during suckling modestly influenced the microbiome in adulthood, which might partially contribute to the adverse/beneficial effects, respectively, of these conditions on programmed metabolic health. |
| first_indexed | 2024-03-08T15:31:11Z |
| format | Article |
| id | doaj.art-f0862c16e7d14893af8cd069eb19817e |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-03-08T15:31:11Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-f0862c16e7d14893af8cd069eb19817e2024-01-10T05:26:21ZengWileyFood Frontiers2643-84292024-01-015110812910.1002/fft2.322Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during sucklingCatalina A. Pomar0Tomeu Viver1Ana Valle2Juan F Gago3Pedro Castillo4Mariona Palou5Andreu Palou6Ramon Rossello‐Mora7Catalina Picó8Laboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics, Biomarkers and Risk Evaluation) University of the Balearic Islands Palma SpainMarine Microbiology Group, Department of Animal and Microbial Diversity IMEDEA (CSIC‐UIB) Esporles Balears SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics, Biomarkers and Risk Evaluation) University of the Balearic Islands Palma SpainMarine Microbiology Group, Department of Animal and Microbial Diversity IMEDEA (CSIC‐UIB) Esporles Balears SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics, Biomarkers and Risk Evaluation) University of the Balearic Islands Palma SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics, Biomarkers and Risk Evaluation) University of the Balearic Islands Palma SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics, Biomarkers and Risk Evaluation) University of the Balearic Islands Palma SpainMarine Microbiology Group, Department of Animal and Microbial Diversity IMEDEA (CSIC‐UIB) Esporles Balears SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics, Biomarkers and Risk Evaluation) University of the Balearic Islands Palma SpainAbstract Early life conditions may influence the gut microbiome and contribute to health status in adulthood. We examined the impact of maternal obesity and/or obesogenic diet on the gut microbiome of mothers (at weaning) and their adult offspring, along with the effects of leptin supplementation during suckling. Three groups of rats were studied: control (C) dams, fed with standard diet (SD); western diet (WD) dams, fed with WD (a high‐fat, high‐sucrose diet) before gestation and during gestation and lactation; and reversion (REV) dams, fed as WD‐dams but with SD during lactation. Offspring were supplemented throughout suckling with leptin/vehicle and weaned on an SD. WD‐dams showed lower microbial diversity and an altered microbiome compared to C‐ and REV‐dams, widely normalized by diet improvement during lactation. WD‐ and REV‐offspring (males) displayed lower microbiome diversity and greater dominance compared to C‐offspring. Taxonomic group differences (Proteobacteria phylum and Bacteroides, Parasutterella, and Phocea genera) were observed in WD‐ and REV‐offspring. WD‐offspring, but not REV‐offspring, had a greater abundance of the phylum Firmicutes and Lachnospiraceae family and a lower abundance of the family Tannerellaceae compared to C‐offspring. Leptin supplementation led to decreased abundance of the phylum Actinobacteria (family Eggerthellaceae and genera Enterorhabdus and Adlercreutzia (females)), and the genera Lachnospiraceae UCG‐008 group, Roseburia and Limosilactobacillus, and increased abundance of the genus Dellaglioa, compared to vehicle‐treated groups. Therefore, maternal consumption of an obesogenic diet during the perinatal period results in microbiome changes in dams, alleviated by dietary improvement during lactation. Maternal conditions and leptin supplementation during suckling modestly influenced the microbiome in adulthood, which might partially contribute to the adverse/beneficial effects, respectively, of these conditions on programmed metabolic health.https://doi.org/10.1002/fft2.322leptinmaternal obesitymaternal overnutritionmetabolic programingmicrobiome |
| spellingShingle | Catalina A. Pomar Tomeu Viver Ana Valle Juan F Gago Pedro Castillo Mariona Palou Andreu Palou Ramon Rossello‐Mora Catalina Picó Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling Food Frontiers leptin maternal obesity maternal overnutrition metabolic programing microbiome |
| title | Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling |
| title_full | Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling |
| title_fullStr | Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling |
| title_full_unstemmed | Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling |
| title_short | Western diet strongly influences gut microbiomes in nursing rat dams but translates modest long‐term consequences to offspring modulated by leptin supplementation during suckling |
| title_sort | western diet strongly influences gut microbiomes in nursing rat dams but translates modest long term consequences to offspring modulated by leptin supplementation during suckling |
| topic | leptin maternal obesity maternal overnutrition metabolic programing microbiome |
| url | https://doi.org/10.1002/fft2.322 |
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