Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation

Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation

In Mammals, microglial cells are considered as the resident immune cells in central nervous system (CNS). Many studies demonstrated that, after injury, these cells are activated and recruited at the lesion site. Leech microglia present a similar pattern of microglial activation and migration upon ex...

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Bibliographic Details
Main Authors: T Arab, A Raffo-Romero, C Van Camp, Q Lemaire, F Le Marrec-Croq, F Drago, S Aboulouard, C Slomianny, A-S Lacoste, I Guigon, H Touzet, M Salzet, I Fournier, C Lefebvre, J Vizioli, P-E Sautière
Format: Article
Language:English
Published: Wiley 2019-12-01
Series:Journal of Extracellular Vesicles
Subjects:
hirudo medicinalis
microglia
extracellular vesicles
protein content
ultracentrifugation
optiprep™
neurite outgrowth
Online Access:http://dx.doi.org/10.1080/20013078.2019.1603048

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