Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini
The phytochemical profile of the methanolic extracts (pulp and peel) obtained from two dehydration methods (drying and freeze-lyophilization) of the traditional Italian apple Mela Rosa dei Monti Sibillini, as well as their inhibitory properties against some biological enzymes (α-glucosidase, lipase,...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/13/6/127 |
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| author | Víctor López Francisco Les Serena Mevi Joice Guileine Nkuimi Wandjou Guillermo Cásedas Giovanni Caprioli Filippo Maggi |
| author_facet | Víctor López Francisco Les Serena Mevi Joice Guileine Nkuimi Wandjou Guillermo Cásedas Giovanni Caprioli Filippo Maggi |
| author_sort | Víctor López |
| collection | DOAJ |
| description | The phytochemical profile of the methanolic extracts (pulp and peel) obtained from two dehydration methods (drying and freeze-lyophilization) of the traditional Italian apple Mela Rosa dei Monti Sibillini, as well as their inhibitory properties against some biological enzymes (α-glucosidase, lipase, monoamine oxidase A, tyrosinase and acetylcholinesterase) were assessed in this study. HPLC-DAD-MS technique was used for the determination of polyphenolic and triterpenic compounds. The determination of the enzymes inhibitory effect was made through spectrophotometric techniques. The peel extracts were richer in bioactive compounds than the pulp. In this regard, the extracts from freeze-lyophilization displayed higher levels of flavan-3-ols, flavonol glycosides and dihydrochalcones. However, the extracts obtained from dried material displayed a stronger enzyme inhibition. Notably, the peel extracts showed a higher activity than the pulp ones, especially in terms of α-glucosidase whereby some samples exerted a similar enzymatic inhibition than acarbose (100% inhibition) at high concentrations (1 mg/mL). These results encourage thus further studies on this traditional Italian apple as a potential source of nutraceuticals helpful to prevent the insurgence of some pathologies. |
| first_indexed | 2024-03-10T18:58:49Z |
| format | Article |
| id | doaj.art-f08bf79f47bc4ec2ab3bc291a6ad4c7c |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T18:58:49Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-f08bf79f47bc4ec2ab3bc291a6ad4c7c2023-11-20T04:35:23ZengMDPI AGPharmaceuticals1424-82472020-06-0113612710.3390/ph13060127Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti SibilliniVíctor López0Francisco Les1Serena Mevi2Joice Guileine Nkuimi Wandjou3Guillermo Cásedas4Giovanni Caprioli5Filippo Maggi6Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830 Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830 Zaragoza, SpainSchool of Pharmacy, University of Camerino, 62032 Camerino, ItalySchool of Pharmacy, University of Camerino, 62032 Camerino, ItalyDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830 Zaragoza, SpainSchool of Pharmacy, University of Camerino, 62032 Camerino, ItalySchool of Pharmacy, University of Camerino, 62032 Camerino, ItalyThe phytochemical profile of the methanolic extracts (pulp and peel) obtained from two dehydration methods (drying and freeze-lyophilization) of the traditional Italian apple Mela Rosa dei Monti Sibillini, as well as their inhibitory properties against some biological enzymes (α-glucosidase, lipase, monoamine oxidase A, tyrosinase and acetylcholinesterase) were assessed in this study. HPLC-DAD-MS technique was used for the determination of polyphenolic and triterpenic compounds. The determination of the enzymes inhibitory effect was made through spectrophotometric techniques. The peel extracts were richer in bioactive compounds than the pulp. In this regard, the extracts from freeze-lyophilization displayed higher levels of flavan-3-ols, flavonol glycosides and dihydrochalcones. However, the extracts obtained from dried material displayed a stronger enzyme inhibition. Notably, the peel extracts showed a higher activity than the pulp ones, especially in terms of α-glucosidase whereby some samples exerted a similar enzymatic inhibition than acarbose (100% inhibition) at high concentrations (1 mg/mL). These results encourage thus further studies on this traditional Italian apple as a potential source of nutraceuticals helpful to prevent the insurgence of some pathologies.https://www.mdpi.com/1424-8247/13/6/127polyphenolstriterpenesantioxidantsenzyme inhibitorsapplefunctional foods |
| spellingShingle | Víctor López Francisco Les Serena Mevi Joice Guileine Nkuimi Wandjou Guillermo Cásedas Giovanni Caprioli Filippo Maggi Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini Pharmaceuticals polyphenols triterpenes antioxidants enzyme inhibitors apple functional foods |
| title | Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini |
| title_full | Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini |
| title_fullStr | Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini |
| title_full_unstemmed | Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini |
| title_short | Phytochemicals and Enzyme Inhibitory Capacities of the Methanolic Extracts from the Italian Apple Cultivar Mela Rosa dei Monti Sibillini |
| title_sort | phytochemicals and enzyme inhibitory capacities of the methanolic extracts from the italian apple cultivar mela rosa dei monti sibillini |
| topic | polyphenols triterpenes antioxidants enzyme inhibitors apple functional foods |
| url | https://www.mdpi.com/1424-8247/13/6/127 |
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