Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China
Returning straw to the field, coupled with fertilizer application, is an effective means to improve the fertility of black soil in Northeast China. Previous studies have mainly focused on the physical and chemical properties of soil structure and fertility. However, few efforts have been made to stu...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/8/2036 |
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| author | Feng Jiao Dongdong Zhang Yang Chen Jinhua Wu Junying Zhang |
| author_facet | Feng Jiao Dongdong Zhang Yang Chen Jinhua Wu Junying Zhang |
| author_sort | Feng Jiao |
| collection | DOAJ |
| description | Returning straw to the field, coupled with fertilizer application, is an effective means to improve the fertility of black soil in Northeast China. Previous studies have mainly focused on the physical and chemical properties of soil structure and fertility. However, few efforts have been made to study the impact of straw returning on the microbial community of black soil in Northeast China. Here, we studied the typical northeast black soil in Heilongjiang Province to characterize the effects of long-term chemical fertilizer application and straw returning on its bacterial community structure. High-throughput sequencing was conducted to characterize the bacterial community of northeast black soil under different agricultural fertilization treatments, and the main factors affecting the bacterial community of northeast black soil were revealed through bioinformatic analyses. The results of high-throughput sequencing analyses demonstrated that the main bacterial phyla in the black soil in Northeast China were Actinomycetes, Proteobacteria, Acidobacteria, Chloroflexus, and Bacteroidetes. Long-term application of chemical fertilizers significantly increased the fertility and crop yield of black soil in Northeast China but led to significant changes in bacterial community structure and a significant decrease in diversity. Although straw returning improved soil fertility, it did not alleviate the adverse effects of the long-term application of chemical fertilizers on soil bacterial communities. Furthermore, our results demonstrated that changes in soil pH were the main factor leading to variations in soil bacterial communities. Returning straw to the field based on fertilizer application can improve black soil fertility in Northeast China but fails to alleviate the adverse effects of fertilizer-induced soil acidification on the composition and diversity of soil bacterial communities. This suggests that returning straw to the field may not have a significant beneficial impact on the microbial ecology of the black soil of Northeast China. Therefore, further research is needed to establish new straw return strategies to maximize agricultural yields while minimizing ecological impacts. |
| first_indexed | 2024-03-11T00:13:00Z |
| format | Article |
| id | doaj.art-f08c584203f34914bcec1184a1ac9414 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T00:13:00Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-f08c584203f34914bcec1184a1ac94142023-11-18T23:54:08ZengMDPI AGAgronomy2073-43952023-07-01138203610.3390/agronomy13082036Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast ChinaFeng Jiao0Dongdong Zhang1Yang Chen2Jinhua Wu3Junying Zhang4College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaReturning straw to the field, coupled with fertilizer application, is an effective means to improve the fertility of black soil in Northeast China. Previous studies have mainly focused on the physical and chemical properties of soil structure and fertility. However, few efforts have been made to study the impact of straw returning on the microbial community of black soil in Northeast China. Here, we studied the typical northeast black soil in Heilongjiang Province to characterize the effects of long-term chemical fertilizer application and straw returning on its bacterial community structure. High-throughput sequencing was conducted to characterize the bacterial community of northeast black soil under different agricultural fertilization treatments, and the main factors affecting the bacterial community of northeast black soil were revealed through bioinformatic analyses. The results of high-throughput sequencing analyses demonstrated that the main bacterial phyla in the black soil in Northeast China were Actinomycetes, Proteobacteria, Acidobacteria, Chloroflexus, and Bacteroidetes. Long-term application of chemical fertilizers significantly increased the fertility and crop yield of black soil in Northeast China but led to significant changes in bacterial community structure and a significant decrease in diversity. Although straw returning improved soil fertility, it did not alleviate the adverse effects of the long-term application of chemical fertilizers on soil bacterial communities. Furthermore, our results demonstrated that changes in soil pH were the main factor leading to variations in soil bacterial communities. Returning straw to the field based on fertilizer application can improve black soil fertility in Northeast China but fails to alleviate the adverse effects of fertilizer-induced soil acidification on the composition and diversity of soil bacterial communities. This suggests that returning straw to the field may not have a significant beneficial impact on the microbial ecology of the black soil of Northeast China. Therefore, further research is needed to establish new straw return strategies to maximize agricultural yields while minimizing ecological impacts.https://www.mdpi.com/2073-4395/13/8/2036Mollisolsfertilizercommunity structurehigh-throughput sequencingbacterial diversity |
| spellingShingle | Feng Jiao Dongdong Zhang Yang Chen Jinhua Wu Junying Zhang Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China Agronomy Mollisols fertilizer community structure high-throughput sequencing bacterial diversity |
| title | Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China |
| title_full | Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China |
| title_fullStr | Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China |
| title_full_unstemmed | Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China |
| title_short | Effects of Long-Term Straw Returning and Nitrogen Fertilizer Reduction on Soil Microbial Diversity in Black Soil in Northeast China |
| title_sort | effects of long term straw returning and nitrogen fertilizer reduction on soil microbial diversity in black soil in northeast china |
| topic | Mollisols fertilizer community structure high-throughput sequencing bacterial diversity |
| url | https://www.mdpi.com/2073-4395/13/8/2036 |
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