The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research
Scopul lucrarii: De a demonstra ca acidul sialic poate fi utilizat ca marker tumoral in monitorizarea evolutiei melanomului la modelele experimentale. Datele obtinute s-au extrapolat la om, astfel incat acest marker sa poata fi folosit in practica medicala. Material si metode: S-au utilizat linii ce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2013-06-01
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| Series: | Romanian Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/rrlm-2013-0012 |
| _version_ | 1830514952584036352 |
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| author | Lazescu Andreea Veronica D. Gruia Maria Iuliana I. Anghel Rodica M. Glavan Daniela A. |
| author_facet | Lazescu Andreea Veronica D. Gruia Maria Iuliana I. Anghel Rodica M. Glavan Daniela A. |
| author_sort | Lazescu Andreea Veronica D. |
| collection | DOAJ |
| description | Scopul lucrarii: De a demonstra ca acidul sialic poate fi utilizat ca marker tumoral in monitorizarea evolutiei melanomului la modelele experimentale. Datele obtinute s-au extrapolat la om, astfel incat acest marker sa poata fi folosit in practica medicala. Material si metode: S-au utilizat linii celulare experimentale tip B16 de melanom. Lotul a fost alcatuit din 30 de soareci masculi de tip C57Bl6 care au primit un numar de 5x105 celule tumorale prin injectare in flancul drept. Volumul tumoral a fost masurat cu un caliper vernier. Acidul sialic s-a determinat din ser obtinut prin punctie cardiaca. A doua parte a studiului s-a desfasurat pe un numar de 25 de pacienti cu melanom. Acidul sialic s-a determinat prin metoda colorimetrica Katterman. Corelatia dintre progresia bolii si acidul sialic a fost ulterior exemplificata prin doua cazuri clinice. Determinarea acidului sialic s-a efectuat in dinamica progresiei bolii. Rezultate: La modelele murine cresterea tumorala s-a inregistrat dupa o perioada de lag de 10 zile si s-a evidentiat prin masurarea diametrelor si volumelor tumorale. Am observat o crestere direct proportionala a acidului sialic. La subiectii umani nivelele serice au crescut in boala metastatica si au ramas normale in boala localizata. In cele doua cazuri clinice s-a observat o corelatie stransa intre progresia bolii si acidul sialic. Concluzii: celulele B16 de melanoma au potential inalt de metastazare. Nivelele de acid sialic sunt crescute la soarecii cu metastaze. Nivelele crescute de acid sialic se coreleaza cu potentialul metastatic al tumorii. La oameni, acidul sialic creste in fazele avansate ale bolii si nu are utilitate in depistare sau screening. |
| first_indexed | 2024-12-22T03:12:54Z |
| format | Article |
| id | doaj.art-f08dca31dd484e87b626f3b2001a39f8 |
| institution | Directory Open Access Journal |
| issn | 2284-5623 |
| language | English |
| last_indexed | 2024-12-22T03:12:54Z |
| publishDate | 2013-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Romanian Journal of Laboratory Medicine |
| spelling | doaj.art-f08dca31dd484e87b626f3b2001a39f82022-12-21T18:40:53ZengSciendoRomanian Journal of Laboratory Medicine2284-56232013-06-0121217918810.2478/rrlm-2013-0012The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human researchLazescu Andreea Veronica D.0Gruia Maria Iuliana I.1Anghel Rodica M.2Glavan Daniela A.3University of Medicine and Pharmacy “Carol Davila” Bucharest, Oncology Department, Institute of Oncology “Prof. Dr. Alexandru Trestioreanu”, Fundeni Road no.252, district 2, Bucharest. Fax: (+40) 021/2271242, Mobile phone: +40721336483Institute of Oncology “Prof. Dr. Alexandru Trestioreanu” BucharestUniversity of Medicine and Pharmacy “Carol Davila” Bucharest, Oncology DepartmentInstitute of Oncology “Prof. Dr. Alexandru Trestioreanu” BucharestScopul lucrarii: De a demonstra ca acidul sialic poate fi utilizat ca marker tumoral in monitorizarea evolutiei melanomului la modelele experimentale. Datele obtinute s-au extrapolat la om, astfel incat acest marker sa poata fi folosit in practica medicala. Material si metode: S-au utilizat linii celulare experimentale tip B16 de melanom. Lotul a fost alcatuit din 30 de soareci masculi de tip C57Bl6 care au primit un numar de 5x105 celule tumorale prin injectare in flancul drept. Volumul tumoral a fost masurat cu un caliper vernier. Acidul sialic s-a determinat din ser obtinut prin punctie cardiaca. A doua parte a studiului s-a desfasurat pe un numar de 25 de pacienti cu melanom. Acidul sialic s-a determinat prin metoda colorimetrica Katterman. Corelatia dintre progresia bolii si acidul sialic a fost ulterior exemplificata prin doua cazuri clinice. Determinarea acidului sialic s-a efectuat in dinamica progresiei bolii. Rezultate: La modelele murine cresterea tumorala s-a inregistrat dupa o perioada de lag de 10 zile si s-a evidentiat prin masurarea diametrelor si volumelor tumorale. Am observat o crestere direct proportionala a acidului sialic. La subiectii umani nivelele serice au crescut in boala metastatica si au ramas normale in boala localizata. In cele doua cazuri clinice s-a observat o corelatie stransa intre progresia bolii si acidul sialic. Concluzii: celulele B16 de melanoma au potential inalt de metastazare. Nivelele de acid sialic sunt crescute la soarecii cu metastaze. Nivelele crescute de acid sialic se coreleaza cu potentialul metastatic al tumorii. La oameni, acidul sialic creste in fazele avansate ale bolii si nu are utilitate in depistare sau screening.https://doi.org/10.2478/rrlm-2013-0012melanomasialic acidmarkermurine modelsmetastaticmelanomacid sialicmarkermodele murinemetastaze |
| spellingShingle | Lazescu Andreea Veronica D. Gruia Maria Iuliana I. Anghel Rodica M. Glavan Daniela A. The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research Romanian Journal of Laboratory Medicine melanoma sialic acid marker murine models metastatic melanom acid sialic marker modele murine metastaze |
| title | The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research |
| title_full | The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research |
| title_fullStr | The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research |
| title_full_unstemmed | The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research |
| title_short | The role of the sialic acid in monitoring the evolution of malignant melanoma. From murine models to human research |
| title_sort | role of the sialic acid in monitoring the evolution of malignant melanoma from murine models to human research |
| topic | melanoma sialic acid marker murine models metastatic melanom acid sialic marker modele murine metastaze |
| url | https://doi.org/10.2478/rrlm-2013-0012 |
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