Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients
Monika Buraczynska, Jakub Golacki, Wojciech Zaluska Department of Nephrology, Medical University of Lublin, Lublin, PolandCorrespondence: Monika Buraczynska, Department of Nephrology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-950, Poland, Tel +48 81 7244716, Fax +48 81 7244357, Email...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-03-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
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| Online Access: | https://www.dovepress.com/leu72met-polymorphism-in-ghrelin-gene-a-potential-risk-factor-for-hype-peer-reviewed-fulltext-article-DMSO |
| _version_ | 1811161174077603840 |
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| author | Buraczynska M Golacki J Zaluska W |
| author_facet | Buraczynska M Golacki J Zaluska W |
| author_sort | Buraczynska M |
| collection | DOAJ |
| description | Monika Buraczynska, Jakub Golacki, Wojciech Zaluska Department of Nephrology, Medical University of Lublin, Lublin, PolandCorrespondence: Monika Buraczynska, Department of Nephrology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-950, Poland, Tel +48 81 7244716, Fax +48 81 7244357, Email monika.buraczynska@umlub.plObjective: Ghrelin (GHRL) is known to be engaged in metabolic and cardiovascular processes. There is evidence suggesting its involvement in the regulation of blood pressure and hypertension. The purpose of this preliminary case–control study was to determine the involvement of the Leu72Met (rs696217) polymorphism in the GHRL gene in type 2 diabetes (T2DM).Methods: The Leu72Met polymorphism was genotyped in 820 individuals with T2DM and 400 healthy subjects by the PCR-RFLP technique. The polymorphism distribution was first compared in those withT2DM and controls, then in subgroups of participants representing different clinical phenotypes.Results: No significant association was identified between Leu72Met and T2DM. The distribution of polymorphism was analyzed in subgroups of individuals with different clinical phenotypes (hypertension, diabetic nephropathy, obesity). In this analysis, rs696217 was associated with hypertension. The presence of T allele was associated with higher risk of hypertension (OR = 2.50, 95% CI 1.68– 3.73, p < 0.001). When adjusted for age, gender and BMI, the association was still significant (OR = 2.62, 95% CI 1.83– 3.96, p < 0.001). A post hoc power calculations based on a minor allele frequency revealed the power of 97% for comparison between HY+ and HY- subgroups.Conclusion: This is the first study demonstrating that the ghrelin Leu72Met SNP is associated with hypertension in Caucasians with T2DM. If confirmed in larger studies in different populations, it may be a novel potential risk factor for hypertension in individuals withT2DM.Keywords: ghrelin gene, type 2 diabetes, hypertension, Leu72Met polymorphism, risk allele |
| first_indexed | 2024-04-10T06:11:15Z |
| format | Article |
| id | doaj.art-f0942e8db648405b907a2bf3d49a17f6 |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T06:11:15Z |
| publishDate | 2023-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-f0942e8db648405b907a2bf3d49a17f62023-03-02T18:11:56ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072023-03-01Volume 1655756481954Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes PatientsBuraczynska MGolacki JZaluska WMonika Buraczynska, Jakub Golacki, Wojciech Zaluska Department of Nephrology, Medical University of Lublin, Lublin, PolandCorrespondence: Monika Buraczynska, Department of Nephrology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-950, Poland, Tel +48 81 7244716, Fax +48 81 7244357, Email monika.buraczynska@umlub.plObjective: Ghrelin (GHRL) is known to be engaged in metabolic and cardiovascular processes. There is evidence suggesting its involvement in the regulation of blood pressure and hypertension. The purpose of this preliminary case–control study was to determine the involvement of the Leu72Met (rs696217) polymorphism in the GHRL gene in type 2 diabetes (T2DM).Methods: The Leu72Met polymorphism was genotyped in 820 individuals with T2DM and 400 healthy subjects by the PCR-RFLP technique. The polymorphism distribution was first compared in those withT2DM and controls, then in subgroups of participants representing different clinical phenotypes.Results: No significant association was identified between Leu72Met and T2DM. The distribution of polymorphism was analyzed in subgroups of individuals with different clinical phenotypes (hypertension, diabetic nephropathy, obesity). In this analysis, rs696217 was associated with hypertension. The presence of T allele was associated with higher risk of hypertension (OR = 2.50, 95% CI 1.68– 3.73, p < 0.001). When adjusted for age, gender and BMI, the association was still significant (OR = 2.62, 95% CI 1.83– 3.96, p < 0.001). A post hoc power calculations based on a minor allele frequency revealed the power of 97% for comparison between HY+ and HY- subgroups.Conclusion: This is the first study demonstrating that the ghrelin Leu72Met SNP is associated with hypertension in Caucasians with T2DM. If confirmed in larger studies in different populations, it may be a novel potential risk factor for hypertension in individuals withT2DM.Keywords: ghrelin gene, type 2 diabetes, hypertension, Leu72Met polymorphism, risk allelehttps://www.dovepress.com/leu72met-polymorphism-in-ghrelin-gene-a-potential-risk-factor-for-hype-peer-reviewed-fulltext-article-DMSOghrelin genetype 2 diabeteshypertensionleu72met polymorphismrisk allele |
| spellingShingle | Buraczynska M Golacki J Zaluska W Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients Diabetes, Metabolic Syndrome and Obesity ghrelin gene type 2 diabetes hypertension leu72met polymorphism risk allele |
| title | Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients |
| title_full | Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients |
| title_fullStr | Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients |
| title_full_unstemmed | Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients |
| title_short | Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients |
| title_sort | leu72met polymorphism in ghrelin gene a potential risk factor for hypertension in type 2 diabetes patients |
| topic | ghrelin gene type 2 diabetes hypertension leu72met polymorphism risk allele |
| url | https://www.dovepress.com/leu72met-polymorphism-in-ghrelin-gene-a-potential-risk-factor-for-hype-peer-reviewed-fulltext-article-DMSO |
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