LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs
The spleen plays a central role in human and canine visceral leishmaniasis, where the activation of the immune response occurs in one of the tissues where <i>Leishmania infantum</i> reproduces. Therefore, this organ is both a target to understand the mechanisms involved in the parasite c...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/9/974 |
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| author | Bruno Mendes Roatt Jamille Mirelle de Oliveira Cardoso Levi Eduardo Soares Reis Gabriel José Lucas Moreira Letícia Captein Gonçalves Flávia de Souza Marques Nádia das Dores Moreira Paula Melo de Abreu Vieira Rodrigo Dian de Oliveira Aguiar-Soares Rodolfo Cordeiro Giunchetti Alexandre Barbosa Reis |
| author_facet | Bruno Mendes Roatt Jamille Mirelle de Oliveira Cardoso Levi Eduardo Soares Reis Gabriel José Lucas Moreira Letícia Captein Gonçalves Flávia de Souza Marques Nádia das Dores Moreira Paula Melo de Abreu Vieira Rodrigo Dian de Oliveira Aguiar-Soares Rodolfo Cordeiro Giunchetti Alexandre Barbosa Reis |
| author_sort | Bruno Mendes Roatt |
| collection | DOAJ |
| description | The spleen plays a central role in human and canine visceral leishmaniasis, where the activation of the immune response occurs in one of the tissues where <i>Leishmania infantum</i> reproduces. Therefore, this organ is both a target to understand the mechanisms involved in the parasite control and a parameter for assessing the therapeutic response. In this sense, this study aimed to evaluate the main histological, immunological and parasitological aspects in the spleen of symptomatic dogs naturally infected by <i>L. infantum</i> treated with the therapeutic vaccine LBMPL. For this, dogs were divided into four groups: dogs uninfected and untreated (NI group); <i>L. infantum</i>-infected dogs that were not treated (INT group); <i>L. infantum</i>-infected dogs that received treatment only with monophosphoryl lipid A adjuvant (MPL group); and <i>L. infantum</i>-infected dogs that received treatment with the vaccine composed by <i>L. braziliensis</i> promastigote proteins associated with MPL adjuvant (LBMPL group). Ninety days after the therapeutics protocol, the dogs were euthanized and the spleen was collected for the proposed evaluations. Our results demonstrated a reduction of hyperplasia of red pulp and follicular area of white pulp, increased mRNA expression of IFN-γ, TNF-α, IL-12 and iNOS, and decreased IL-10 and TGF-β1, and intense reduction of splenic parasitism in dogs treated with the LBMPL vaccine. These results possibly suggest that the pro-inflammatory environment promoted the progressive organization of the splenic architecture favoring the cellular activation, with consequent parasite control. Along with previously obtained data, our results propose the LBMPL vaccine as a possible treatment strategy for canine visceral leishmaniasis (CVL). |
| first_indexed | 2024-03-09T22:53:45Z |
| format | Article |
| id | doaj.art-f095a2609a044c1a96ff027b4b959f2b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T22:53:45Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-f095a2609a044c1a96ff027b4b959f2b2023-11-23T18:15:18ZengMDPI AGPathogens2076-08172022-08-0111997410.3390/pathogens11090974LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected DogsBruno Mendes Roatt0Jamille Mirelle de Oliveira Cardoso1Levi Eduardo Soares Reis2Gabriel José Lucas Moreira3Letícia Captein Gonçalves4Flávia de Souza Marques5Nádia das Dores Moreira6Paula Melo de Abreu Vieira7Rodrigo Dian de Oliveira Aguiar-Soares8Rodolfo Cordeiro Giunchetti9Alexandre Barbosa Reis10Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Morfopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilFaculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 21941-902, Rio de Janeiro, BrazilDepartamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilLaboratório de Biologia das Interações Celulares, Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte 31270-013, Minas Gerais, BrazilLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Minas Gerais, BrazilThe spleen plays a central role in human and canine visceral leishmaniasis, where the activation of the immune response occurs in one of the tissues where <i>Leishmania infantum</i> reproduces. Therefore, this organ is both a target to understand the mechanisms involved in the parasite control and a parameter for assessing the therapeutic response. In this sense, this study aimed to evaluate the main histological, immunological and parasitological aspects in the spleen of symptomatic dogs naturally infected by <i>L. infantum</i> treated with the therapeutic vaccine LBMPL. For this, dogs were divided into four groups: dogs uninfected and untreated (NI group); <i>L. infantum</i>-infected dogs that were not treated (INT group); <i>L. infantum</i>-infected dogs that received treatment only with monophosphoryl lipid A adjuvant (MPL group); and <i>L. infantum</i>-infected dogs that received treatment with the vaccine composed by <i>L. braziliensis</i> promastigote proteins associated with MPL adjuvant (LBMPL group). Ninety days after the therapeutics protocol, the dogs were euthanized and the spleen was collected for the proposed evaluations. Our results demonstrated a reduction of hyperplasia of red pulp and follicular area of white pulp, increased mRNA expression of IFN-γ, TNF-α, IL-12 and iNOS, and decreased IL-10 and TGF-β1, and intense reduction of splenic parasitism in dogs treated with the LBMPL vaccine. These results possibly suggest that the pro-inflammatory environment promoted the progressive organization of the splenic architecture favoring the cellular activation, with consequent parasite control. Along with previously obtained data, our results propose the LBMPL vaccine as a possible treatment strategy for canine visceral leishmaniasis (CVL).https://www.mdpi.com/2076-0817/11/9/974canine visceral leishmaniasis<i>Leishmania infantum</i>immunotherapyLBMPL vaccinespleen |
| spellingShingle | Bruno Mendes Roatt Jamille Mirelle de Oliveira Cardoso Levi Eduardo Soares Reis Gabriel José Lucas Moreira Letícia Captein Gonçalves Flávia de Souza Marques Nádia das Dores Moreira Paula Melo de Abreu Vieira Rodrigo Dian de Oliveira Aguiar-Soares Rodolfo Cordeiro Giunchetti Alexandre Barbosa Reis LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs Pathogens canine visceral leishmaniasis <i>Leishmania infantum</i> immunotherapy LBMPL vaccine spleen |
| title | LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs |
| title_full | LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs |
| title_fullStr | LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs |
| title_full_unstemmed | LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs |
| title_short | LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism in <i>Leishmania infantum</i> Naturally Infected Dogs |
| title_sort | lbmpl vaccine therapy induces progressive organization of the spleen microarchitecture improved th1 adaptative immune response and control of parasitism in i leishmania infantum i naturally infected dogs |
| topic | canine visceral leishmaniasis <i>Leishmania infantum</i> immunotherapy LBMPL vaccine spleen |
| url | https://www.mdpi.com/2076-0817/11/9/974 |
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