DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI
A total of 229 strains of actinomycetes were isolated and identified by full sequence of 16S rRNA gene analysis. Samples consisted of 18 soil and 20 leaf-litter were collected from Eka Karya Botanical Garden, Bali Island, Indonesia. Two isolation methods, i.e. SDS-Yeast Extract (SY) and Rehydration...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SEAMEO, BIOTROP
2016-08-01
|
| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/504 |
| _version_ | 1827902876434825216 |
|---|---|
| author | Shanti Ratnakomala Puspita Lisdiyanti Nita Rosalinda Prayitno Evi Triana Yulin Lestari Ratih Dwi Hastuti Yantyati Widyastuti Misa Otoguro Katsuhiko Ando Endang Sukara |
| author_facet | Shanti Ratnakomala Puspita Lisdiyanti Nita Rosalinda Prayitno Evi Triana Yulin Lestari Ratih Dwi Hastuti Yantyati Widyastuti Misa Otoguro Katsuhiko Ando Endang Sukara |
| author_sort | Shanti Ratnakomala |
| collection | DOAJ |
| description |
A total of 229 strains of actinomycetes were isolated and identified by full sequence of 16S rRNA gene analysis. Samples consisted of 18 soil and 20 leaf-litter were collected from Eka Karya Botanical Garden, Bali Island, Indonesia. Two isolation methods, i.e. SDS-Yeast Extract (SY) and Rehydration-Centrifugation (RC) were used in this study. Based on 16S rRNA gene analysis, isolated actinomycetes may be grouped into 28 genera. Based on molecular analysis of 16S rRNA gene similarities showed that isolated actinomycetes of Eka Karya Botanical Garden origin is diverse. Analysis on 144 isolates from soil samples, resulted in 24 genera and more than 87 species. Streptomyces is the most dominant genus where 65 isolates or 45% from isolated actinomycetes belong to this genus. It was followed by Actinoplanes (25 isolates =17%). From leaf-littersamples, the total number of 85 isolates may be grouped into 9 genera and more than 41 species. The most dominated genus is Actinoplanes (42 isolates =49%) followed by Catenuloplanes (16 isolates=19%).
Keywords: 16S rRNA gene analysis, actinomycetes, biodiversity, Eka Karya Botanical Garden
|
| first_indexed | 2024-03-13T00:05:21Z |
| format | Article |
| id | doaj.art-f097471d514c4f4eac5dffdf637a04c5 |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-03-13T00:05:21Z |
| publishDate | 2016-08-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-f097471d514c4f4eac5dffdf637a04c52023-07-13T04:58:16ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2016-08-0123110.11598/btb.2016.23.1.504DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALIShanti Ratnakomala0Puspita Lisdiyanti1Nita Rosalinda Prayitno2Evi Triana3Yulin Lestari4Ratih Dwi Hastuti5Yantyati Widyastuti6Misa Otoguro7Katsuhiko Ando8Endang Sukara9Research Center for Biotechnology Indonesian Institute of SciencesResearch Center for Biotechnology Indonesian Institute of SciencesUniversity Health Network - Toronto General HospitalResearch Center for Biology Indonesian Institute of SciencesDepartement of Biology, Faculty of Mathematics and Natural Sciences, IPB, Darmaga Campus, BogorSoil Research Institute, Bogor 16002Research Center for Biotechnology Indonesian Institute of SciencesUniversity of YamanashiNITE Biologycal Resource Center (NBRC)Research Center for Biotechnology Indonesian Institute of Sciences A total of 229 strains of actinomycetes were isolated and identified by full sequence of 16S rRNA gene analysis. Samples consisted of 18 soil and 20 leaf-litter were collected from Eka Karya Botanical Garden, Bali Island, Indonesia. Two isolation methods, i.e. SDS-Yeast Extract (SY) and Rehydration-Centrifugation (RC) were used in this study. Based on 16S rRNA gene analysis, isolated actinomycetes may be grouped into 28 genera. Based on molecular analysis of 16S rRNA gene similarities showed that isolated actinomycetes of Eka Karya Botanical Garden origin is diverse. Analysis on 144 isolates from soil samples, resulted in 24 genera and more than 87 species. Streptomyces is the most dominant genus where 65 isolates or 45% from isolated actinomycetes belong to this genus. It was followed by Actinoplanes (25 isolates =17%). From leaf-littersamples, the total number of 85 isolates may be grouped into 9 genera and more than 41 species. The most dominated genus is Actinoplanes (42 isolates =49%) followed by Catenuloplanes (16 isolates=19%). Keywords: 16S rRNA gene analysis, actinomycetes, biodiversity, Eka Karya Botanical Garden https://journal.biotrop.org/index.php/biotropia/article/view/504 |
| spellingShingle | Shanti Ratnakomala Puspita Lisdiyanti Nita Rosalinda Prayitno Evi Triana Yulin Lestari Ratih Dwi Hastuti Yantyati Widyastuti Misa Otoguro Katsuhiko Ando Endang Sukara DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI Biotropia: The Southeast Asian Journal of Tropical Biology |
| title | DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI |
| title_full | DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI |
| title_fullStr | DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI |
| title_full_unstemmed | DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI |
| title_short | DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI |
| title_sort | diversity of actinomycetes from eka karya botanical garden bali |
| url | https://journal.biotrop.org/index.php/biotropia/article/view/504 |
| work_keys_str_mv | AT shantiratnakomala diversityofactinomycetesfromekakaryabotanicalgardenbali AT puspitalisdiyanti diversityofactinomycetesfromekakaryabotanicalgardenbali AT nitarosalindaprayitno diversityofactinomycetesfromekakaryabotanicalgardenbali AT evitriana diversityofactinomycetesfromekakaryabotanicalgardenbali AT yulinlestari diversityofactinomycetesfromekakaryabotanicalgardenbali AT ratihdwihastuti diversityofactinomycetesfromekakaryabotanicalgardenbali AT yantyatiwidyastuti diversityofactinomycetesfromekakaryabotanicalgardenbali AT misaotoguro diversityofactinomycetesfromekakaryabotanicalgardenbali AT katsuhikoando diversityofactinomycetesfromekakaryabotanicalgardenbali AT endangsukara diversityofactinomycetesfromekakaryabotanicalgardenbali |