In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies
Background: We assumed that the pharmacokinetics of lovastatin could be changed by the induction effect of berberine. Methods: An UPLC-MS/MS method was developed and validated for the pharmacokinetics tudy of lovastatin to investigate the in vivo drug-drug interactions between lovastatin and berberi...
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| Format: | Article |
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MDPI AG
2016-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/4/464 |
| _version_ | 1819162373255069696 |
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| author | Hanming Cui Jialong Wang Qiuyan Zhang Mengmeng Dang Hui Liu Yu Dong Lu Zhang Fang Yang Jianhua Wu Xiaolin Tong |
| author_facet | Hanming Cui Jialong Wang Qiuyan Zhang Mengmeng Dang Hui Liu Yu Dong Lu Zhang Fang Yang Jianhua Wu Xiaolin Tong |
| author_sort | Hanming Cui |
| collection | DOAJ |
| description | Background: We assumed that the pharmacokinetics of lovastatin could be changed by the induction effect of berberine. Methods: An UPLC-MS/MS method was developed and validated for the pharmacokinetics tudy of lovastatin to investigate the in vivo drug-drug interactions between lovastatin and berberine. SD male rats were random divided into lovastatin group and berberine induced prior to lovastatin group for the in vivo pharmacokinetic studies. Meanwhile HepG2 cells were induced by berberine for three days to study the metabolism of lovastatin. Results: The AUC (p < 0.01) and Cmax (p < 0.01) could be significantly decreased in the berberine-induced group in vivo, and the metabolic activity of HepG2 cell ccould be increased by berberine induction in vitro. The metabolism parameters of lovastatin such as CL, Vmax and Km were increased after the induction of berberine. From the pharmacokinetic study of lovastatin induced with berberine, we obtained pharmacokinetic parameters which are compliance with the metabolic parameters of lovastatin in HepG2 cells with berberine induction in vitro. Conclusions: From the in vivo pharmacokinetics study and the HepG2 cell metabolism study in vitro, berberine could be an inducer for the metabolism of lovastatin according to our previous research on berberine induction effects on HepG2 cells, which may be relevant to the fact that berberine possesses induction effects through the CYP 450 3A4 enzyme. |
| first_indexed | 2024-12-22T17:27:12Z |
| format | Article |
| id | doaj.art-f097aeae4b674fa9860ecd1ff3398b40 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T17:27:12Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f097aeae4b674fa9860ecd1ff3398b402022-12-21T18:18:42ZengMDPI AGMolecules1420-30492016-04-0121446410.3390/molecules21040464molecules21040464In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism StudiesHanming Cui0Jialong Wang1Qiuyan Zhang2Mengmeng Dang3Hui Liu4Yu Dong5Lu Zhang6Fang Yang7Jianhua Wu8Xiaolin Tong9Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaGuang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaGuang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaCollege of Pharmacy, Shannxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaCollege of Pharmacy, Shannxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaGuang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaCollege of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaDepartment of Agriculture and Life Sciences, Ankang University, Ankang 725000, ChinaCollege of Pharmacy, Shannxi University of Traditional Chinese Medicine, Xianyang 712046, ChinaGuang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaBackground: We assumed that the pharmacokinetics of lovastatin could be changed by the induction effect of berberine. Methods: An UPLC-MS/MS method was developed and validated for the pharmacokinetics tudy of lovastatin to investigate the in vivo drug-drug interactions between lovastatin and berberine. SD male rats were random divided into lovastatin group and berberine induced prior to lovastatin group for the in vivo pharmacokinetic studies. Meanwhile HepG2 cells were induced by berberine for three days to study the metabolism of lovastatin. Results: The AUC (p < 0.01) and Cmax (p < 0.01) could be significantly decreased in the berberine-induced group in vivo, and the metabolic activity of HepG2 cell ccould be increased by berberine induction in vitro. The metabolism parameters of lovastatin such as CL, Vmax and Km were increased after the induction of berberine. From the pharmacokinetic study of lovastatin induced with berberine, we obtained pharmacokinetic parameters which are compliance with the metabolic parameters of lovastatin in HepG2 cells with berberine induction in vitro. Conclusions: From the in vivo pharmacokinetics study and the HepG2 cell metabolism study in vitro, berberine could be an inducer for the metabolism of lovastatin according to our previous research on berberine induction effects on HepG2 cells, which may be relevant to the fact that berberine possesses induction effects through the CYP 450 3A4 enzyme.http://www.mdpi.com/1420-3049/21/4/464in vivo and in vitroberberinelovastatinpharmacokineticsHepG2 cell |
| spellingShingle | Hanming Cui Jialong Wang Qiuyan Zhang Mengmeng Dang Hui Liu Yu Dong Lu Zhang Fang Yang Jianhua Wu Xiaolin Tong In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies Molecules in vivo and in vitro berberine lovastatin pharmacokinetics HepG2 cell |
| title | In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies |
| title_full | In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies |
| title_fullStr | In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies |
| title_full_unstemmed | In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies |
| title_short | In Vivo and in Vitro Study on Drug-Drug Interaction of Lovastatin and Berberine from Pharmacokinetic and HepG2 Cell Metabolism Studies |
| title_sort | in vivo and in vitro study on drug drug interaction of lovastatin and berberine from pharmacokinetic and hepg2 cell metabolism studies |
| topic | in vivo and in vitro berberine lovastatin pharmacokinetics HepG2 cell |
| url | http://www.mdpi.com/1420-3049/21/4/464 |
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