Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model
Abstract This study aimed to evaluate the antioxidant potential of the Coffea arabica Lineu (L.) leaf extract and its effects on platelet aggregation of dyslipidemic rats. The extract was obtained by the percolation of C. arabica L. leaves in hydroethanolic solution 70% (v/v). The mass spectrometry...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-08-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100673&lng=en&tlng=en |
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| author | Mario Henrique Souza Simões Bruno Cesar Correa Salles Stella Maris da Silveira Duarte Marcelo Aparecido da Silva André Luiz Machado Viana Gabriel de Oliveira Isaac de Moraes Sonia Aparecida Figueiredo Eric Batista Ferreira Maria Rita Rodrigues Fernanda Borges de Araújo Paula |
| author_facet | Mario Henrique Souza Simões Bruno Cesar Correa Salles Stella Maris da Silveira Duarte Marcelo Aparecido da Silva André Luiz Machado Viana Gabriel de Oliveira Isaac de Moraes Sonia Aparecida Figueiredo Eric Batista Ferreira Maria Rita Rodrigues Fernanda Borges de Araújo Paula |
| author_sort | Mario Henrique Souza Simões |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the antioxidant potential of the Coffea arabica Lineu (L.) leaf extract and its effects on platelet aggregation of dyslipidemic rats. The extract was obtained by the percolation of C. arabica L. leaves in hydroethanolic solution 70% (v/v). The mass spectrometry FIA-ESI-MS² suggested the presence of chlorogenic acid, rutin acid, and quinic acid. The DPPH• radicals scavenging capacity was demonstrated (IC50 = 0.06 mg/mL). The extract was administered to rats by gavage (300 mg/kg/day) for 56 days. Dyslipidemia was induced by administering Triton WR-1339 (300 mg/kg body weight) on the 54th day. On day 56, blood was collected by puncturing the abdominal aorta artery and the aortic artery was removed. Lipid profile, markers of renal and hepatic injury, lipid peroxidation, and platelet aggregation tests were carried out. The ingestion of extract reduced the lipid peroxidation (aorta and plasma) and platelet aggregation in dyslipidemic rats. The extract did not affect markers of renal and hepatic function as analyzed in this study, suggesting neither impaired liver nor kidney function in these animals. Therefore, our results demonstrate that the extract of leaves of C. arabica L. show antioxidant potential in vitro and in vivo as well as anti-platelet aggregation in dyslipidemic animals. |
| first_indexed | 2024-04-11T21:21:45Z |
| format | Article |
| id | doaj.art-f09a13ee4c6548f4ba7f4ad74488df26 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-11T21:21:45Z |
| publishDate | 2022-08-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-f09a13ee4c6548f4ba7f4ad74488df262022-12-22T04:02:36ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-08-015810.1590/s2175-97902022e19562Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia modelMario Henrique Souza SimõesBruno Cesar Correa SallesStella Maris da Silveira DuarteMarcelo Aparecido da SilvaAndré Luiz Machado VianaGabriel de Oliveira Isaac de MoraesSonia Aparecida FigueiredoEric Batista FerreiraMaria Rita RodriguesFernanda Borges de Araújo Paulahttps://orcid.org/0000-0003-3077-3023Abstract This study aimed to evaluate the antioxidant potential of the Coffea arabica Lineu (L.) leaf extract and its effects on platelet aggregation of dyslipidemic rats. The extract was obtained by the percolation of C. arabica L. leaves in hydroethanolic solution 70% (v/v). The mass spectrometry FIA-ESI-MS² suggested the presence of chlorogenic acid, rutin acid, and quinic acid. The DPPH• radicals scavenging capacity was demonstrated (IC50 = 0.06 mg/mL). The extract was administered to rats by gavage (300 mg/kg/day) for 56 days. Dyslipidemia was induced by administering Triton WR-1339 (300 mg/kg body weight) on the 54th day. On day 56, blood was collected by puncturing the abdominal aorta artery and the aortic artery was removed. Lipid profile, markers of renal and hepatic injury, lipid peroxidation, and platelet aggregation tests were carried out. The ingestion of extract reduced the lipid peroxidation (aorta and plasma) and platelet aggregation in dyslipidemic rats. The extract did not affect markers of renal and hepatic function as analyzed in this study, suggesting neither impaired liver nor kidney function in these animals. Therefore, our results demonstrate that the extract of leaves of C. arabica L. show antioxidant potential in vitro and in vivo as well as anti-platelet aggregation in dyslipidemic animals.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100673&lng=en&tlng=enCoffeeAntioxidantDyslipidemiaLipid peroxidationPlatelet aggregation |
| spellingShingle | Mario Henrique Souza Simões Bruno Cesar Correa Salles Stella Maris da Silveira Duarte Marcelo Aparecido da Silva André Luiz Machado Viana Gabriel de Oliveira Isaac de Moraes Sonia Aparecida Figueiredo Eric Batista Ferreira Maria Rita Rodrigues Fernanda Borges de Araújo Paula Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model Brazilian Journal of Pharmaceutical Sciences Coffee Antioxidant Dyslipidemia Lipid peroxidation Platelet aggregation |
| title | Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model |
| title_full | Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model |
| title_fullStr | Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model |
| title_full_unstemmed | Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model |
| title_short | Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model |
| title_sort | leaf extract of coffea arabica l reduces lipid peroxidation and has anti platelet effect in a rat dyslipidemia model |
| topic | Coffee Antioxidant Dyslipidemia Lipid peroxidation Platelet aggregation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100673&lng=en&tlng=en |
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