Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans
Ligusticum chuanxiong Hort. (CX) is a medicinal and edible plant including a variety of active substances, which may be an available resource for the treatment of related diseases. To expand the medicinal uses of CX, this study aims to explore the antioxidant, anti-aging and neuroprotective effects...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1049890/full |
| _version_ | 1828139554359476224 |
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| author | Yihan Qin Fangfang Chen Zizhong Tang Hongjiao Ren Qing Wang Nayu Shen Wenjie Lin Yirong Xiao Ming Yuan Hui Chen Tongliang Bu Qingfeng Li Lin Huang |
| author_facet | Yihan Qin Fangfang Chen Zizhong Tang Hongjiao Ren Qing Wang Nayu Shen Wenjie Lin Yirong Xiao Ming Yuan Hui Chen Tongliang Bu Qingfeng Li Lin Huang |
| author_sort | Yihan Qin |
| collection | DOAJ |
| description | Ligusticum chuanxiong Hort. (CX) is a medicinal and edible plant including a variety of active substances, which may be an available resource for the treatment of related diseases. To expand the medicinal uses of CX, this study aims to explore the antioxidant, anti-aging and neuroprotective effects of the Ligusticum chuanxiong leaves (CXL) and rhizome (CXR) extracts. We first characterize CX phytochemical spectrum by LC-MS as well as antioxidant capacity. Acute toxicity, anti-oxidative stress capacity, lifespan and healthspan was evaluated in C elegans N2. Neuroprotective effect was evaluated in vitro and in vivo (C elegans CL4176 and CL2355). In this study, we detected 74 and 78 compounds from CXR and CXL, respectively, including phthalides, alkaloids, organic acids, terpenes, polyphenols and others. Furthermore, we found that CXs not only protect against oxidative stress, but also prolong the lifespan, alleviate lipofuscin, malondialdehyde (MDA) and reactive oxygen species (ROS) accumulation, and improve movement level, antioxidant enzyme activity in C elegans N2. However, only CXR reduced the β-amyloid peptide (Aβ)-induced paralysis phenotype in CL4176s and alleviated chemosensory behavior dysfunction in CL2355s. In addition, CXR treatment reduced the production of Aβ and ROS, enhanced SOD activity in CL4176s. The possible mechanism of anti-aging of CXL and CXR is to promote the expression of related antioxidant pathway genes, increase the activity of antioxidant enzymes, and reduce the accumulation of ROS, which is dependent on DAF-16 and HSF-1 (only in CXR). CXR was able to activate antioxidase-related (sod-3 and sod-5) and heat shock protein genes (hsp-16.1 and hsp-70) expression, consequently ameliorating proteotoxicity related to Aβ aggregation. In summary, these findings demonstrate the antioxidant, anti-aging and neuroprotective (only in CXR) activities of the CX, which provide an important pharmacological basis for developing functional foods and drugs to relieve the symptoms of aging and AD. However, the material basis of neuroprotective activity and antiaging effects need to be elucidated, and the relationship between these activities should also be clarified in future studies. |
| first_indexed | 2024-04-11T18:54:43Z |
| format | Article |
| id | doaj.art-f09bce92b3dc4ebfa4ed49293dcd9847 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T18:54:43Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-f09bce92b3dc4ebfa4ed49293dcd98472022-12-22T04:08:15ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-10-011310.3389/fphar.2022.10498901049890Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegansYihan Qin0Fangfang Chen1Zizhong Tang2Hongjiao Ren3Qing Wang4Nayu Shen5Wenjie Lin6Yirong Xiao7Ming Yuan8Hui Chen9Tongliang Bu10Qingfeng Li11Lin Huang12College of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaSichuan Agricultural University Hospital, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaCollege of Life Sciences, Sichuan Agricultural University, Ya’an, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaLigusticum chuanxiong Hort. (CX) is a medicinal and edible plant including a variety of active substances, which may be an available resource for the treatment of related diseases. To expand the medicinal uses of CX, this study aims to explore the antioxidant, anti-aging and neuroprotective effects of the Ligusticum chuanxiong leaves (CXL) and rhizome (CXR) extracts. We first characterize CX phytochemical spectrum by LC-MS as well as antioxidant capacity. Acute toxicity, anti-oxidative stress capacity, lifespan and healthspan was evaluated in C elegans N2. Neuroprotective effect was evaluated in vitro and in vivo (C elegans CL4176 and CL2355). In this study, we detected 74 and 78 compounds from CXR and CXL, respectively, including phthalides, alkaloids, organic acids, terpenes, polyphenols and others. Furthermore, we found that CXs not only protect against oxidative stress, but also prolong the lifespan, alleviate lipofuscin, malondialdehyde (MDA) and reactive oxygen species (ROS) accumulation, and improve movement level, antioxidant enzyme activity in C elegans N2. However, only CXR reduced the β-amyloid peptide (Aβ)-induced paralysis phenotype in CL4176s and alleviated chemosensory behavior dysfunction in CL2355s. In addition, CXR treatment reduced the production of Aβ and ROS, enhanced SOD activity in CL4176s. The possible mechanism of anti-aging of CXL and CXR is to promote the expression of related antioxidant pathway genes, increase the activity of antioxidant enzymes, and reduce the accumulation of ROS, which is dependent on DAF-16 and HSF-1 (only in CXR). CXR was able to activate antioxidase-related (sod-3 and sod-5) and heat shock protein genes (hsp-16.1 and hsp-70) expression, consequently ameliorating proteotoxicity related to Aβ aggregation. In summary, these findings demonstrate the antioxidant, anti-aging and neuroprotective (only in CXR) activities of the CX, which provide an important pharmacological basis for developing functional foods and drugs to relieve the symptoms of aging and AD. However, the material basis of neuroprotective activity and antiaging effects need to be elucidated, and the relationship between these activities should also be clarified in future studies.https://www.frontiersin.org/articles/10.3389/fphar.2022.1049890/fullLigusticum chuanxiongantioxidant activityantiaging activityneuroprotective activityCaenorhabditis elegans |
| spellingShingle | Yihan Qin Fangfang Chen Zizhong Tang Hongjiao Ren Qing Wang Nayu Shen Wenjie Lin Yirong Xiao Ming Yuan Hui Chen Tongliang Bu Qingfeng Li Lin Huang Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans Frontiers in Pharmacology Ligusticum chuanxiong antioxidant activity antiaging activity neuroprotective activity Caenorhabditis elegans |
| title | Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans |
| title_full | Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans |
| title_fullStr | Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans |
| title_full_unstemmed | Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans |
| title_short | Ligusticum chuanxiong Hort as a medicinal and edible plant foods: Antioxidant, anti-aging and neuroprotective properties in Caenorhabditis elegans |
| title_sort | ligusticum chuanxiong hort as a medicinal and edible plant foods antioxidant anti aging and neuroprotective properties in caenorhabditis elegans |
| topic | Ligusticum chuanxiong antioxidant activity antiaging activity neuroprotective activity Caenorhabditis elegans |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1049890/full |
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