Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study
The objective of the study was to validate our previous results obtained during the transcriptome analysis of the primary culture of peripheral blood macrophages in patients with Parkinson's disease associated with mutations in the GBA gene (GBA-PD) in that reduced expression of the neurogenesi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Academician I.P. Pavlov First St. Petersburg State Medical University
2022-07-01
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| Series: | Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова |
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| Online Access: | https://www.sci-notes.ru/jour/article/view/871 |
| _version_ | 1797702330846543872 |
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| author | A. I. Bezrukova K. S. Basharova I. V. Miliukhina A. A. Timofeeva K. A. Senkevich S. N. Pchelina T. S. Usenko |
| author_facet | A. I. Bezrukova K. S. Basharova I. V. Miliukhina A. A. Timofeeva K. A. Senkevich S. N. Pchelina T. S. Usenko |
| author_sort | A. I. Bezrukova |
| collection | DOAJ |
| description | The objective of the study was to validate our previous results obtained during the transcriptome analysis of the primary culture of peripheral blood macrophages in patients with Parkinson's disease associated with mutations in the GBA gene (GBA-PD) in that reduced expression of the neurogenesis genes EGR1 (early growth response protein 1), NR4A2 (nuclear receptor 4A2), JUNB (transcription factor jun-B) in patients with GBA-PD.Methods and materials. The study included 14 patients with GBA-PD, 15 GBA-carriers, 30 patients with Parkinson's disease (PD) and 44 persons of the control group. The assessment of relative mRNA level of neurogenesis genes EGR1, NR4A2, JUNB in peripheral blood mononuclear cells were carried out by real-time quantitative polymerase chain reaction (PCR) using TaqMan fluorescent probes or EvaGreen fluorescent DNA dye.Results. Relative mRNA level of the JUNB gene in peripheral blood mononuclears was decreased in the group of patients with GBA-PD compared to controls (p=0.034). We found out that the relative mRNA level of the NR4A2 gene in peripheral blood mononuclears was increased in the group of patients with GBA-carriers compared to GBA-PD, patients with PD and controls (p=0.0029, p=0.00045, p=0.0024 respectively). There were no statistically significant differences in the mRNA level of the EGR1 gene between all the study groups (p>0.05).Conclusion. GBA-PD is characterized by reduced expression of the JUNB gene compared to control and of the NR4A2 gene compared to GBA-carriers. |
| first_indexed | 2024-03-12T04:49:27Z |
| format | Article |
| id | doaj.art-f09de606945041a1912502103af8acf2 |
| institution | Directory Open Access Journal |
| issn | 1607-4181 |
| language | Russian |
| last_indexed | 2024-03-12T04:49:27Z |
| publishDate | 2022-07-01 |
| publisher | Academician I.P. Pavlov First St. Petersburg State Medical University |
| record_format | Article |
| series | Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова |
| spelling | doaj.art-f09de606945041a1912502103af8acf22023-09-03T09:26:34ZrusAcademician I.P. Pavlov First St. Petersburg State Medical UniversityУчёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова1607-41812022-07-01291374510.24884/1607-4181-2022-29-1-37-45592Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome studyA. I. Bezrukova0K. S. Basharova1I. V. Miliukhina2A. A. Timofeeva3K. A. Senkevich4S. N. Pchelina5T. S. Usenko6Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»Pavlov University; N.P. Bekhtereva Institute of the Human BrainPavlov UniversityPavlov UniversityPetersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»; Pavlov UniversityPetersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»; Pavlov UniversityThe objective of the study was to validate our previous results obtained during the transcriptome analysis of the primary culture of peripheral blood macrophages in patients with Parkinson's disease associated with mutations in the GBA gene (GBA-PD) in that reduced expression of the neurogenesis genes EGR1 (early growth response protein 1), NR4A2 (nuclear receptor 4A2), JUNB (transcription factor jun-B) in patients with GBA-PD.Methods and materials. The study included 14 patients with GBA-PD, 15 GBA-carriers, 30 patients with Parkinson's disease (PD) and 44 persons of the control group. The assessment of relative mRNA level of neurogenesis genes EGR1, NR4A2, JUNB in peripheral blood mononuclear cells were carried out by real-time quantitative polymerase chain reaction (PCR) using TaqMan fluorescent probes or EvaGreen fluorescent DNA dye.Results. Relative mRNA level of the JUNB gene in peripheral blood mononuclears was decreased in the group of patients with GBA-PD compared to controls (p=0.034). We found out that the relative mRNA level of the NR4A2 gene in peripheral blood mononuclears was increased in the group of patients with GBA-carriers compared to GBA-PD, patients with PD and controls (p=0.0029, p=0.00045, p=0.0024 respectively). There were no statistically significant differences in the mRNA level of the EGR1 gene between all the study groups (p>0.05).Conclusion. GBA-PD is characterized by reduced expression of the JUNB gene compared to control and of the NR4A2 gene compared to GBA-carriers.https://www.sci-notes.ru/jour/article/view/871parkinson's diseasegene expressionneurogenesisperipheral blood mononuclear cells |
| spellingShingle | A. I. Bezrukova K. S. Basharova I. V. Miliukhina A. A. Timofeeva K. A. Senkevich S. N. Pchelina T. S. Usenko Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова parkinson's disease gene expression neurogenesis peripheral blood mononuclear cells |
| title | Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study |
| title_full | Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study |
| title_fullStr | Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study |
| title_full_unstemmed | Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study |
| title_short | Reduced expression of neurogenesis genes as biomarkers of Parkinson's disease associated with mutations in the <i>GBA</i> gene: validation of the data analysis of transcriptome study |
| title_sort | reduced expression of neurogenesis genes as biomarkers of parkinson s disease associated with mutations in the i gba i gene validation of the data analysis of transcriptome study |
| topic | parkinson's disease gene expression neurogenesis peripheral blood mononuclear cells |
| url | https://www.sci-notes.ru/jour/article/view/871 |
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