Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity
Introduction: Obesity is commonly linked up with several life-threatening diseases. This study aims to investigate the association of fatty acid synthase (FASN) rs4246445, rs2229425, rs2228305, and rs2229422 single nucleotide polymorphisms (SNPs) with the risk of overweight and obesity in the Malays...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2018-05-01
|
| Series: | BioImpacts |
| Subjects: | |
| Online Access: | https://bi.tbzmed.ac.ir/PDF/bi-8-159.pdf |
| _version_ | 1818650422756245504 |
|---|---|
| author | Eric Tzyy Jiann Chong Shawn Shi Erh Kuok Ping-Chin Lee |
| author_facet | Eric Tzyy Jiann Chong Shawn Shi Erh Kuok Ping-Chin Lee |
| author_sort | Eric Tzyy Jiann Chong |
| collection | DOAJ |
| description | Introduction: Obesity is commonly linked up with several life-threatening diseases. This study aims to investigate the association of fatty acid synthase (FASN) rs4246445, rs2229425, rs2228305, and rs2229422 single nucleotide polymorphisms (SNPs) with the risk of overweight and obesity in the Malaysian population. Methods: Blood samples were collected from 1030 individuals who were grouped into normal, overweight, and obese categories. Blood biochemistry test and lipid profiling were performed and genomic DNA was extracted. Genotyping was performed using hydrolysis probes and odd ratio with 95% CI was calculated for risk association analysis. Linkage disequilibrium and haplotypes analyses were carried out using SHEsis software. Results: We found that the hemoglobin and white blood cell counts were significantly high in the obese subjects. There is a lack of evidence to link the FASN SNPs with the risk of overweight and obesity in the population. All 4 SNPs were seemed to be in linkage equilibrium. Five common haplotypes were identified in this study but none of them was significantly associated with overweight and obesity in the population. Conclusion: Our findings suggest a lack of evidence to associate the FASN rs4246445, rs2229425, rs2228305, and rs2229422 SNPs with the risk of overweight and obesity in the Malaysian population. All 4 SNPs were independent of each other and not all identified haplotypes were significantly associated with overweight and obesity in this study. |
| first_indexed | 2024-12-17T01:49:58Z |
| format | Article |
| id | doaj.art-f09f42f66a2e4da18a5ae9aec13c545b |
| institution | Directory Open Access Journal |
| issn | 2228-5660 2228-5652 |
| language | English |
| last_indexed | 2024-12-17T01:49:58Z |
| publishDate | 2018-05-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj.art-f09f42f66a2e4da18a5ae9aec13c545b2022-12-21T22:08:05ZengTabriz University of Medical SciencesBioImpacts2228-56602228-56522018-05-018315916510.15171/bi.2018.18bi-17460Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesityEric Tzyy Jiann Chong0Shawn Shi Erh Kuok1Ping-Chin Lee2Biotechnology Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaBiotechnology Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaBiotechnology Programme, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaIntroduction: Obesity is commonly linked up with several life-threatening diseases. This study aims to investigate the association of fatty acid synthase (FASN) rs4246445, rs2229425, rs2228305, and rs2229422 single nucleotide polymorphisms (SNPs) with the risk of overweight and obesity in the Malaysian population. Methods: Blood samples were collected from 1030 individuals who were grouped into normal, overweight, and obese categories. Blood biochemistry test and lipid profiling were performed and genomic DNA was extracted. Genotyping was performed using hydrolysis probes and odd ratio with 95% CI was calculated for risk association analysis. Linkage disequilibrium and haplotypes analyses were carried out using SHEsis software. Results: We found that the hemoglobin and white blood cell counts were significantly high in the obese subjects. There is a lack of evidence to link the FASN SNPs with the risk of overweight and obesity in the population. All 4 SNPs were seemed to be in linkage equilibrium. Five common haplotypes were identified in this study but none of them was significantly associated with overweight and obesity in the population. Conclusion: Our findings suggest a lack of evidence to associate the FASN rs4246445, rs2229425, rs2228305, and rs2229422 SNPs with the risk of overweight and obesity in the Malaysian population. All 4 SNPs were independent of each other and not all identified haplotypes were significantly associated with overweight and obesity in this study.https://bi.tbzmed.ac.ir/PDF/bi-8-159.pdffatty acid synthaseobesityoverweightsingle nucleotide polymorphisms |
| spellingShingle | Eric Tzyy Jiann Chong Shawn Shi Erh Kuok Ping-Chin Lee Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity BioImpacts fatty acid synthase obesity overweight single nucleotide polymorphisms |
| title | Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity |
| title_full | Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity |
| title_fullStr | Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity |
| title_full_unstemmed | Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity |
| title_short | Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity |
| title_sort | risk association linkage disequilibrium and haplotype analyses of fasn rs4246445 rs2229425 rs2228305 and rs2229422 polymorphisms in overweight and obesity |
| topic | fatty acid synthase obesity overweight single nucleotide polymorphisms |
| url | https://bi.tbzmed.ac.ir/PDF/bi-8-159.pdf |
| work_keys_str_mv | AT erictzyyjiannchong riskassociationlinkagedisequilibriumandhaplotypeanalysesoffasnrs4246445rs2229425rs2228305andrs2229422polymorphismsinoverweightandobesity AT shawnshierhkuok riskassociationlinkagedisequilibriumandhaplotypeanalysesoffasnrs4246445rs2229425rs2228305andrs2229422polymorphismsinoverweightandobesity AT pingchinlee riskassociationlinkagedisequilibriumandhaplotypeanalysesoffasnrs4246445rs2229425rs2228305andrs2229422polymorphismsinoverweightandobesity |