Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax)
Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513421002039 |
| _version_ | 1819240849619288064 |
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| author | Pedro L. Castro Sergio Plasencia María Jesús Zamorano Luis Guerrero Anna Claret José A. Beltrán Juan Calanche Rafael Ginés |
| author_facet | Pedro L. Castro Sergio Plasencia María Jesús Zamorano Luis Guerrero Anna Claret José A. Beltrán Juan Calanche Rafael Ginés |
| author_sort | Pedro L. Castro |
| collection | DOAJ |
| description | Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen (Col Iα2) of large European sea bass (initial body weight 609.21 ± 75.39 g) from high plant-protein diets. Four isoproteic (42 % crude protein) and isolipidic (20 % crude lipid) experimental diets were formulated adding 0.6 (HL diet), 1.2 (HM diet), and 2% (HH diet) L-Hyp, respectively. Three periods of feeding of 45, 99 and 143 days were studied. L-Hyp supplementation at 1.2 % and 2 % significantly improved specific growth rate (SGR) and feed conversion ratio (FCR). In the white muscle, type I, IV collagen and trichromic stain were significantly higher in HH feed than the control diet. In the red muscle, only type I collagen was higher. HH diet, also increases Col Iα2 mRNA levels in muscle significantly. It can be concluded that the addition of crystalline l-Hyp at 1.2 % or 2 % in high plant-protein diets indicates positive effects on growth performance of adult European sea bass and increase in muscle total collagen deposition. |
| first_indexed | 2024-12-23T14:14:33Z |
| format | Article |
| id | doaj.art-f0a0a4c34d8f4bb8bb106918d50ff94b |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-23T14:14:33Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-f0a0a4c34d8f4bb8bb106918d50ff94b2022-12-21T17:43:58ZengElsevierAquaculture Reports2352-51342021-11-0121100787Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax)Pedro L. Castro0Sergio Plasencia1María Jesús Zamorano2Luis Guerrero3Anna Claret4José A. Beltrán5Juan Calanche6Rafael Ginés7GIA-ECOAQUA Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, Telde, 35214, Spain; Corresponding author at: GIA, Grupo de Investigación en Acuicultura, Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, Telde, 35214, Spain.GIA-ECOAQUA Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, Telde, 35214, SpainGIA-ECOAQUA Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, Telde, 35214, SpainIRTA-Food Technology, XaRTA, Finca Camps i Armet s/n, Monells, Girona, E-17121, SpainIRTA-Food Technology, XaRTA, Finca Camps i Armet s/n, Monells, Girona, E-17121, SpainInstituto Agroalimentario de Aragón -IA2- (Universidad de Zaragoza-CITA), Zaragoza, SpainInstituto Agroalimentario de Aragón -IA2- (Universidad de Zaragoza-CITA), Zaragoza, SpainGIA-ECOAQUA Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, Telde, 35214, SpainHydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen (Col Iα2) of large European sea bass (initial body weight 609.21 ± 75.39 g) from high plant-protein diets. Four isoproteic (42 % crude protein) and isolipidic (20 % crude lipid) experimental diets were formulated adding 0.6 (HL diet), 1.2 (HM diet), and 2% (HH diet) L-Hyp, respectively. Three periods of feeding of 45, 99 and 143 days were studied. L-Hyp supplementation at 1.2 % and 2 % significantly improved specific growth rate (SGR) and feed conversion ratio (FCR). In the white muscle, type I, IV collagen and trichromic stain were significantly higher in HH feed than the control diet. In the red muscle, only type I collagen was higher. HH diet, also increases Col Iα2 mRNA levels in muscle significantly. It can be concluded that the addition of crystalline l-Hyp at 1.2 % or 2 % in high plant-protein diets indicates positive effects on growth performance of adult European sea bass and increase in muscle total collagen deposition.http://www.sciencedirect.com/science/article/pii/S2352513421002039European sea bassHydroxyprolineCollagenHistologyTextureGene expression |
| spellingShingle | Pedro L. Castro Sergio Plasencia María Jesús Zamorano Luis Guerrero Anna Claret José A. Beltrán Juan Calanche Rafael Ginés Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax) Aquaculture Reports European sea bass Hydroxyproline Collagen Histology Texture Gene expression |
| title | Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax) |
| title_full | Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax) |
| title_fullStr | Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax) |
| title_full_unstemmed | Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax) |
| title_short | Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax) |
| title_sort | effect of l hyp supplementation on collagen muscle histology gene expression growth performance body composition and fillet texture on big size european sea bass dicentrarchux labrax |
| topic | European sea bass Hydroxyproline Collagen Histology Texture Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2352513421002039 |
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