Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species
Nucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions with the translocon complexes at the outer (TOC) and inner (TIC) plastid membranes. These complexes exist in multiple isoforms in higher plants a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.560701/full |
| _version_ | 1818083805826646016 |
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| author | Álvaro Eseverri Álvaro Eseverri Can Baysal Vicente Medina Teresa Capell Paul Christou Paul Christou Luis M. Rubio Luis M. Rubio Elena Caro Elena Caro |
| author_facet | Álvaro Eseverri Álvaro Eseverri Can Baysal Vicente Medina Teresa Capell Paul Christou Paul Christou Luis M. Rubio Luis M. Rubio Elena Caro Elena Caro |
| author_sort | Álvaro Eseverri |
| collection | DOAJ |
| description | Nucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions with the translocon complexes at the outer (TOC) and inner (TIC) plastid membranes. These complexes exist in multiple isoforms in higher plants and show differential specificity and tissue abundance. While some show specificity for photosynthesis-related precursor proteins, others distinctly recognize nonphotosynthetic and housekeeping precursor proteins. Here we used TPs from four Arabidopsis thaliana proteins, three related to photosynthesis (chlorophyll a/b binding protein, Rubisco activase) and photo-protection (tocopherol cyclase) and one involved in the assimilation of ammonium into amino-acids, and whose expression is most abundant in the root (ferredoxin dependent glutamate synthase 2), to determine whether they were able to mediate import of a nuclear-encoded marker protein into plastids of different tissues of a dicot and a monocot species. In A. thaliana, import and processing efficiency was high in all cases, while TP from the rice Rubisco small chain 1, drove very low import in Arabidopsis tissues. Noteworthy, our results show that Arabidopsis photosynthesis TPs also mediate plastid import in rice callus, and in leaf and root tissues with almost a 100% efficiency, providing new biotechnological tools for crop improvement strategies based on recombinant protein accumulation in plastids by the expression of nuclear-encoded transgenes. |
| first_indexed | 2024-12-10T19:43:50Z |
| format | Article |
| id | doaj.art-f0a3b09e6e754fed8db90cf22677e2ba |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T19:43:50Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f0a3b09e6e754fed8db90cf22677e2ba2022-12-22T01:35:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-09-011110.3389/fpls.2020.560701560701Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different SpeciesÁlvaro Eseverri0Álvaro Eseverri1Can Baysal2Vicente Medina3Teresa Capell4Paul Christou5Paul Christou6Luis M. Rubio7Luis M. Rubio8Elena Caro9Elena Caro10Centre for Plant Biotechnology and Genomics, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Pozuelo de Alarcón, SpainDepartamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, SpainDepartment of Plant Production and Forestry Science, University of Lleida-Agrotecnio Center, Lleida, SpainDepartment of Plant Production and Forestry Science, University of Lleida-Agrotecnio Center, Lleida, SpainDepartment of Plant Production and Forestry Science, University of Lleida-Agrotecnio Center, Lleida, SpainDepartment of Plant Production and Forestry Science, University of Lleida-Agrotecnio Center, Lleida, SpainICREA, Catalan Institute for Research and Advanced Studies, Barcelona, SpainCentre for Plant Biotechnology and Genomics, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Pozuelo de Alarcón, SpainDepartamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, SpainCentre for Plant Biotechnology and Genomics, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Pozuelo de Alarcón, SpainDepartamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, SpainNucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions with the translocon complexes at the outer (TOC) and inner (TIC) plastid membranes. These complexes exist in multiple isoforms in higher plants and show differential specificity and tissue abundance. While some show specificity for photosynthesis-related precursor proteins, others distinctly recognize nonphotosynthetic and housekeeping precursor proteins. Here we used TPs from four Arabidopsis thaliana proteins, three related to photosynthesis (chlorophyll a/b binding protein, Rubisco activase) and photo-protection (tocopherol cyclase) and one involved in the assimilation of ammonium into amino-acids, and whose expression is most abundant in the root (ferredoxin dependent glutamate synthase 2), to determine whether they were able to mediate import of a nuclear-encoded marker protein into plastids of different tissues of a dicot and a monocot species. In A. thaliana, import and processing efficiency was high in all cases, while TP from the rice Rubisco small chain 1, drove very low import in Arabidopsis tissues. Noteworthy, our results show that Arabidopsis photosynthesis TPs also mediate plastid import in rice callus, and in leaf and root tissues with almost a 100% efficiency, providing new biotechnological tools for crop improvement strategies based on recombinant protein accumulation in plastids by the expression of nuclear-encoded transgenes.https://www.frontiersin.org/article/10.3389/fpls.2020.560701/fullplastid targetingrecombinant proteinplastid importtransit peptidericecrops |
| spellingShingle | Álvaro Eseverri Álvaro Eseverri Can Baysal Vicente Medina Teresa Capell Paul Christou Paul Christou Luis M. Rubio Luis M. Rubio Elena Caro Elena Caro Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species Frontiers in Plant Science plastid targeting recombinant protein plastid import transit peptide rice crops |
| title | Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species |
| title_full | Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species |
| title_fullStr | Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species |
| title_full_unstemmed | Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species |
| title_short | Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species |
| title_sort | transit peptides from photosynthesis related proteins mediate import of a marker protein into different plastid types and within different species |
| topic | plastid targeting recombinant protein plastid import transit peptide rice crops |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.560701/full |
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